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Biotechnology and Bioprocess Engineering

, Volume 24, Issue 2, pp 343–358 | Cite as

Method Validation by CPTAC Guidelines for Multi-protein Marker Assays Using Multiple Reaction Monitoring-mass Spectrometry

  • Minsoo Son
  • Hyunsoo Kim
  • Injoon Yeo
  • Yoseop Kim
  • Areum Sohn
  • Youngsoo KimEmail author
Research Paper
  • 7 Downloads

Abstract

Quantifying multiple protein biomarkers in a blood sample at one time has many advantages for diagnosing human diseases. In this study, 34 multiplex assays by multiple reaction monitoring-mass spectrometry (MRM-MS) for serum biomarkers were characterized according to Clinical Proteomic Tumor Analysis Consortium (CPTAC) guidelines. The assays revealed that the median lower limit of quantitation (LLOQ) was 0.37 fmol/μL (16.0 ng/mL) and that the median total coefficient of variation (CV) was 18.2%, 12.2%, and 10.6% in the low-,medium-, and high-quality control (QC) samples. With regard to selectivity, the median mean differences in slope and concentration were 2.1% and 4.3%, respectively. The median values for all CVs and %difference from the nominal concentration for stability were 9.5% and 2.7% in low-QC and 3.8% and 3.1% in medium-QC. The median total CV was 9.8% in the reproducibility. Finally, 17 protein-based biomarker assays were reliable and transferrable for preclinical purposes per CPTAC guidelines.

Keywords

MRM-MS LC-MS/MS CPTAC guidelines method validation quantitative proteomics 

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Notes

Acknowledgements

Hyunsoo Kim and Youngsoo Kim contributed to study concept and design; Minsoo Son and Hyunsoo Kim contributed to acquisition of data; Injoon Yeo contributed to statistical analysis; Youngsoo Kim contributed to obtained funding; Yoseop Kim and Areum Sohn contributed to administrative, technical, or material support; Minsoo Son, Hyunsoo Kim and Youngsoo Kim contributed to drafting of the manuscript. #These authors contributed equally.

This work was supported by the Industrial Strategic Technology Development Program (#10079271 and #2000134) and the Collaborative Genome Program for Fostering New Post-Genome Industry (NRF-2017M3C9A5031597), funded from the Korean Government. This study was also supported by a grant from Seoul National University Hospital (2019).

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer 2019

Authors and Affiliations

  • Minsoo Son
    • 1
  • Hyunsoo Kim
    • 1
    • 2
    • 3
  • Injoon Yeo
    • 1
  • Yoseop Kim
    • 1
  • Areum Sohn
    • 2
  • Youngsoo Kim
    • 1
    • 2
    • 3
    Email author
  1. 1.Departments of Biomedical EngineeringSeoul National University College of MedicineSeoulKorea
  2. 2.Departments of Biomedical SciencesSeoul National University College of MedicineSeoulKorea
  3. 3.Institute of Medical and Biological EngineeringMedical Research CenterSeoulKorea

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