Affinity Peptide-guided Plasmonic Biosensor for Detection of Noroviral Protein and Human Norovirus
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In this study, we developed an affinity peptide-guided plasmonic biosensor that is capable of detection for noroviral capsid proteins and human norovirus. Construction of plasmonic biosensor was achieved by immobilization of affinity peptides (named norovirus binding peptides) on the localized surface plasmonic sensor (LSPR) layer for detection of noroviral capsid proteins and human norovirus. The performance of the plasmonic biosensor in detection of their targets was monitored using LSPR techniques. This specific interaction is proportional to the absorbance of LSPR signals. The lowest detection value for noroviral capsid protein was 0.1 ng/mL in the presence of complex tissue culture media (MEM and FBS), and limit of detection (LOD) for human norovirus was found to be 9.9 copies/mL by the 3-σ rule. Interestingly, no dynamic binding response with norovirus binding peptides as affinity reagent was observed against rotavirus, suggesting that norovirus binding peptides have high selectivity for human norovirus. Thus, norovirus binding peptide-guided plasmonic biosensor could be used for the detection of norovirus-related foodborne pathogens.
Keywordsnorovirus affinity peptide sensitivity plasmonic biosensor limit of detection
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This study of JPP was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (NRF-2011-0010312) and National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) (NRF-2014R1A2A2A01005621, NRF-2017R1A2A2A05001037). Moreover, this research of JPP was also a part of the project titled “Development of portable impedance detection system for food poisoning virus in sea foods”, which was funded by the Ministry of Oceans and Fisheries, Korea. YS Huh gratefully acknowledges financial support from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT CNRF-2018R1A2B2006094). This study of TJP was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01280901)” Rural Development Administration, Republic of Korea.
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