Antioxidant Peptide Purified from Enzymatic Hydrolysates of Isochrysis Zhanjiangensis and Its Protective Effect against Ethanol Induced Oxidative Stress of HepG2 Cells
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Marine microalgae have been widely applied in cosmaceuticals, nutraceuticals, and functional foods. In the present study, we first investigated the hepatoprotective effects of peptide purified from microalgae, Isochrysis zhanjiangensis on HepG2 cells alcoholic injury. I. zhanjiangensis was hydrolyzed utilizing chymotrypsin, trypsin, pepsin, and by vitro gastrointestinal digestion. Among hydrolysates, the gastrointestinal hydrolysate showed relatively high free radical scavenging ability preliminarily and was purified with following sequential chromatography methods. The amino acid sequence and molecular mass of the purified peptide from I. zhanjiangensis (PIZ) was identified as Asn-Asp-Ala-Glu-Tyr-Gly-Ile-Cys-Gly-Phe (NDAEYGICGF; MW, 1088.16 Da) via Q-TOF ESI/MS. Additionally, PIZ attenuated ethanol-induced cytotoxicity and inhibited the production of intracellular reactive oxygen species by DCFH-DA assay. Western blot results showed that superoxide dismutase (SOD) and glutathione (GSH) levels up-regulated with PIZ treatment before alcohol exposure while gamma-glutamyltransferase (GGT) protein expression down-regulated. These results provide an opportunity to discover new highly active peptide against alcohol toxicity in HepG2 cells.
KeywordsIsochrysis zhanjiangensis peptide enzymatic hydrolysates free radical scavenging oxidative stress
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The research was funded by the Yangfan Scarce Top Talent Project of Guangdong Province (201433009) and the Program for Postgraduate Courses and Education Reform and Scientific Research Start-Up Funds of Guangdong Ocean University (to Zhong-Ji Qian). The supported by Guangdong Tongde Pharmaceutical Co., Ltd and National Engineering Research Center for Modernization of Traditional Chinese Medicine (Lingnan Medicinal Plant Oil Branch) and funded by Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1).
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