Biotechnology and Bioprocess Engineering

, Volume 23, Issue 5, pp 500–506 | Cite as

Preparation of in situ Injectable Chitosan/Gelatin Hydrogel Using an Acid-tolerant Tyrosinase

  • Eun Hae Kim
  • Sujin Lim
  • Tae Eun Kim
  • In Oh Jeon
  • Yoo Seong ChoiEmail author
Research Paper


An in situ injectable chitosan/gelatin hydrogel was formed under slightly acidic conditions (pH 4.0 ~ 4.5) using an acid-tolerant tyrosinase, tyrosinase-CNK. A homogeneous chitosan/tyrosinase-CNK solution was prepared in one part of a dual-barrel syringe, and highly soluble gelatin in distilled water was prepared in the other part of the syringe without any additional crosslinking materials. Chitosan/gelatin hydrogel was formed in situ by simple injection of the solutions at room temperature followed by curing at 37°C. However, conventional mushroom tyrosinase did not catalyze this permanent gel formation. Tyrosinase- CNK-catalyzed glycol chitosan/gelatin hydrogel was similarly formed by this in situ injection approach. The hydrogels exhibited a high swelling ratio of 20-fold their own weight, interconnected micropores with an average diameter of approximately 260 μm and in vitro biodegradability suitable for tissue engineering and drug delivery applications. These results showed that tyrosinase-CNK-mediated chitosan/gelatin hydrogel formation has remarkable potential for the development of novel formulations for in situ injectable gel-forming systems.


chitosan gelatin hydrogel in situ injectable gel tyrosinase 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Eun Hae Kim
    • 1
  • Sujin Lim
    • 1
  • Tae Eun Kim
    • 1
  • In Oh Jeon
    • 1
  • Yoo Seong Choi
    • 1
    Email author
  1. 1.Department of Chemical Engineering and Applied ChemistryChungnam National UniversityDaejeonKorea

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