Optimization of Cell Disruption and Transesterification of Lipids from Botryococcus braunii LB572
- 14 Downloads
Several methods including microwave, Frenchpress, autoclave, bead-beating, ultrasonication, and osmotic shock were compared to identify the most effective microalgal cell disruption method. Botryococcus braunii LB572 was cultured in 5 L flasks containing JM medium mixed with oceanic sediment extract for 13 days. Among the methods tested, enhanced lipid extraction was achieved through microwave treatment (2450MHz, 1250W at 150°C for 20 min). Oleic (C18:1), linolenic (C18:3), and palmitic acids (C16:0) were found to be the major fatty acids among the C14-C24 acids from extracted lipid. In addition, the optimal conditions of transesterification were as follows: 70 mL of methanol, 6 mL of sulfuric acid, 8 mL of chloroform, and boiling at 100°C for 30 min; 85.4% of C14-C24 FAME and 78.5% of C16-C18 FAME were esterified from transesterifiable lipids.
KeywordsBotryococcus braunii cell disruption lipid extraction transesterification FAME
Unable to display preview. Download preview PDF.
- 6.Thompson, A. S., J. C. Rhodes, and I. Pettman (1988) Natural environmental research council culture collection of algae and protozoa: catalogue of strains, Freshwater Biological Association. Ambleside, UK.Google Scholar
- 8.Folch, J., M. Lees, and G. H. S. Stanley (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497–509.Google Scholar
- 11.Engler, C. R. (1985) Disruption of microbial cells. pp. 305–324. In: Moo-Young M. (2nd ed.). Comprehensive Biotechnology, Pergamon Press, Oxford. UK.Google Scholar
- 16.Vanderheiden, G. J., A. C. Fairchild, and G. R. Jago (1970) Construction of a laboratory press for use with the French pressure cell. Appl. Microbiol. 19: 875–877.Google Scholar
- 18.Vasavada, P. C. (1986) Effect of microwave energy on bacteria. J. Microwave Power 21: 187–188.Google Scholar
- 20.Zheng, H., J. Yin, Z. Gao, H. Huang, X. Ji, and C. Dou (2011) Disruption of Chlorella vulgaris cells for the release of biodieselproducing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves. Appl. Biochem. Biotechnol. 164: 1215–1224.CrossRefGoogle Scholar