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Biotechnology and Bioprocess Engineering

, Volume 23, Issue 5, pp 580–587 | Cite as

Intergeneric Hybridization between Streptomyces albulus and Bacillus subtilis Facilitates Production of ε-Poly-L-lysine from Corn Starch Residues

  • Shu Li
  • Nan Wang
  • Zong-Jun Du
  • Guan-Jun Chen
Research Paper
  • 6 Downloads

Abstract

Intergeneric hybridization between S.albulus and B. subtilis to produce ε-poly-L-lysine (ε-PL) from corn starch residues (CSR) was investigated in this study. One hybrid, designated S. albulus LS-84, which incorporated the protease gene from B. subtilis, could effectively utilize the protein in CSR as a nitrogen source. In fed-batch fermentation, LS-84 produced 32.6 g/L ε-PL in the presence of 20 g/L CSR. This was an increase of 256.1% compared to that of the parent strain S. albulus LS-01. The rapid hydrolysis of CSR by protease caused rapid growth for LS-84, which allowed higher respiratory activity. As a result, activities of several key enzymes in LS-84 were higher than those in LS-01; additionally, the content of several intracellular amino acids, such as Asp, Glu, and Arg, was also much higher in LS-84. Therefore, intergeneric hybridization between S. albulus and B. subtilis to produce ε-PL from CSR is an economical method for effective utilization of waste resources.

Keywords

ε-poly-L-lysine corn starch residues intergeneric hybridization 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Marine ScienceShandong University (Weihai)WeihaiChina
  2. 2.Weihai Food and Drug AdministrationTesting CenterWeihaiChina
  3. 3.College of Marine ScienceShandong University (Weihai)WeihaiChina

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