Identification of Differentially Expressed Proteins from Smokeless Tobacco Addicted Patients Suffering from Oral Squamous Cell Carcinoma
- 97 Downloads
Oral squamous cell carcinoma (OSCC) is the eight most common malignancy worldwide with an incidence rate of 40% in south-east Asia. Lack of effective diagnostic tools at early stage and disease recurrence despite extensive treatments are main reasons for high mortality and low survival rates. The aim of current study was to identify differentially expressed proteins to explore potential candidate biomarkers having diagnostic significance. We performed comparative proteomic analysis of paired protein samples (cancerous buccal mucosa and adjacent normal tissue) from OSCC patients using a combination of two dimensional gel electrophoresis and Mass spectrometric analysis. On the basis of spot intensity, seventeen proteins were found to be consistently differentially expressed among most of the samples which were identified through mass spectrometry. For validation of identified proteins, expression level of stratifin was determined using immuno-histochemistry and Western blot analysis. All identified proteins were analyzed by STRING to explore their interaction. Among uniquely identified proteins in this study, at least two candidate markers (Ig Kappa chain C region and Isoform 2 of fructose bisphosphate aldolase A) were found to be novel with respect to OSCC which can be explored further. Results presented in current study are likely to contribute in understanding the involvement of these molecules in carcinogenesis apart from their plausible role as diagnostic/prognostic markers.
KeywordsOral squamous cell carcinoma Tissue proteomics Differential expression Biomarker Smokeless tobacco
Financial assistance was provided by Higher Education Commission, Pakistan (Project no. 20-1809). UUM was recipient of EMEA scholarship (SGA 2012-2642) funded by the Erasmus Mundus Programme of EU. Authors acknowledge the technical assistance offered by Kieran Wynne, UCD Conway Institute.
Compliance with Ethical Standards
The study was approved by IRB, (NCP-108), University of Karachi and guidelines proposed by the Declaration of Helsinki were followed.
The work described has not been published previously and the authors declare no competing financial interests.
- 2.Johnson NW, Jayasekara P, Amarasinghe AA (2011) Squamous cell carcinoma and precursor lesions of the oral cavity: epidemiology and aetiology. Periodontol 2000 57:19–37Google Scholar
- 7.de Camargo CM, Voti L, Guerra-Yi M, Chapuis F, Mazuir M, Curado MP (2010) Oral cavity cancer in developed and in developing countries: population-based incidence. Head Neck 32:357–367Google Scholar
- 9.Alamgir M, Jamal Q, Jafarey N, Mirza T (2013) Clinicopathological parameters of 50 oral squamous cell carcinoma cases in Karachi. Pak J Medic Dentis 2:3–8Google Scholar
- 11.G.A.o.t.W.M. Association (2014) World medical association declaration of Helsinki: ethical principles for medical research involving human subjects. J Amer College Dentists 81:14Google Scholar
- 12.Deschler DG, Day T (2008) TNM staging of head and neck cancer and neck dissection classification. Amer Acad Otolaryngol–Head & Neck Surg Found:10–23Google Scholar
- 15.Wang Z, Jiang L, Huang C, Li Z, Chen L, Gou L, Chen P, Tong A, Tang M, Gao F, Shen J, Zhang Y, Bai J, Zhou M, Miao D, Chen Q (2008) Comparative proteomics approach to screening of potential diagnostic and therapeutic targets for oral squamous cell carcinoma. Mol Cell Proteomics 7:1639–1650CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Schmidt M, Hellwig B, Hammad S, Othman A, Lohr M, Chen Z, Boehm D, Gebhard S, Petry I, Lebrecht A, Cadenas C, Marchan R, Stewart JD, Solbach C, Holmberg L, Edlund K, Kultima HG, Rody A, Berglund A, Lambe M, Isaksson A, Botling J, Karn T, Muller V, Gerhold-Ay A, Cotarelo C, Sebastian M, Kronenwett R, Bojar H, Lehr HA, Sahin U, Koelbl H, Gehrmann M, Micke P, Rahnenfuhrer J, Hengstler JG (2012) A comprehensive analysis of human gene expression profiles identifies stromal immunoglobulin κ C as a compatible prognostic marker in human solid tumors. Clin Cancer Res 18:2695–2703CrossRefPubMedGoogle Scholar
- 37.Badea L, Herlea V, Dima SO, Dumitrascu T, Popescu I (2008) Combined gene expression analysis of whole-tissue and microdissected pancreatic ductal adenocarcinoma identifies genes specifically overexpressed in tumor epithelia-the authors reported a combined gene expression analysis of whole-tissue and microdissected pancreatic ductal adenocarcinoma identifies genes specifically overexpressed in tumor epithelia. Hepato-Gastroenterology 55:2016PubMedGoogle Scholar
- 43.Chanthammachat P, Promwikorn W, Pruegsanusak K, Roytrakul S, Srisomsap C, Chokchaichamnankit D, Svasti J, Boonyaphiphat P, K S, Thongsuksai P (2013) Comparative proteomic analysis of oral squamous cell carcinoma and adjacent non-tumour tissue from Thailand. Arch Oral Biol 58:1677–1685CrossRefPubMedGoogle Scholar