C-X-C Chemokine Receptor Type 7 (CXCR-7) Expression in Invasive Ductal Carcinoma of Breast in Association with Clinicopathological Features
C-X-C chemokine receptor type 7 (CXCR-7) is an atypical receptor for chemokines whose role in different stages of carcinogenesis has been evaluated in breast cancer cell lines and animal models. Moreover, it has been demonstrated to be a target of regulation by the tumor suppressor microRNA (miR)-100. In the present study, we assessed CXCR-7 expression in 60 breast cancer patients in association with clinicopathological and demographic data of patients. We also extracted the results of our previous work on miR-100 expression in the same cohort of patients to assess the correlation between miR-100 and CXCR-7 expression levels. Transcript levels of CXCR-7 were significantly higher in tumoral tissues compared with adjacent non-cancerous tissues (ANCTs) (Tumoral vs. ANCTs: 3.64 ± 1.8 vs. 0.73 ± 1.3, P = 0.000). A significant negative correlation was detected between CXCR-7 protein and miR-100 transcript levels (r = −0.526, P < 0.05). High CXCR-7 mRNA levels were significantly associated with tumor size (P = 0.01). Besides, high protein levels were more prevalent in higher TNM stages (P = 0.000). Moreover, high CXCR-7 protein levels were significantly associated with ER (P = 0.005) and PR (P = 0.02) status. The present work provides further evidence for the role of CXCR-7 in breast cancer and proposes the elimination of inhibitory effects of miR-100 on CXCR-7 expression as a mechanism for its up-regulation in breast cancer tissues.
KeywordsCXCR-7 C-X-C chemokine receptor type 7 Breast cancer
This article has been extracted from the thesis written by Roshanak Shamsi in School of Medicine, Shahid Beheshti University of Medical Sciences.
MSA and RS performed the experiments. MB supervised the protein experiments. MDO provided the technical support. SGH designed the study, supervised it and wrote the manuscript.
The present work was financially supported by “Research Department of the School of Medicine Shahid Beheshti University of Medical Sciences”.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no competing interests.
Ethical approval was obtained from the ethical committee of Shahid Beheshti University of Medical Sciences (IR.SBMU.MSP.REC.1396.152). Informed consent was obtained from the patients.
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