The Involvement of NF-κB/Klotho Signaling in Colorectal Cancer Cell Survival and Invasion

  • Biao Xie
  • Shaolin Nie
  • Gui Hu
  • Li Xiong
  • Fan Hu
  • Mei Li
  • Tianshu Peng
  • Jing Nie
  • Yongheng He
Original Article


Lipopolysaccharide significantly increased invasion, cell proliferation, and phospho-NF-κB p65 and phospho-IGF-1R protein, but decreased klotho protein expression, cell apoptosis, and the percentage of sub G0/G1 cells in SW480 and HT29 colorectal cancer cells. In contrast, NF-κB inhibitor exhibited a counteract effect of lipopolysaccharide. Transfection of Toll-like receptor 4 shRNA significantly decreased phospho-NF-κB p65 and phospho-IGF-1R protein levels, invasion, but significantly increased klotho protein expression, cell apoptosis, and the percentage of sub G0/G1 in SW480 and HT29 cells. In conclusion, inflammation inhibits klotho gene expression in colorectal cancer cells through activation of Toll-like receptor 4 /NF-κB signal pathway.


LPS NF-κB Colorectal cancer Klotho 



This study was supported by the Postdoctoral Science Foundation of China (2016 M602416), National Natural Science Foundation of China (81704089), Postdoctoral Science Fund Special Grant of China (2017 T100603), 2016 Postdoctoral Researcher Daily Funding of Hunan Province (49), the Department of Education Key Project of Hunan Province (15A024), and Changsha City’s Outstanding Innovation Youth Training Program (kq 1707012).

Compliance with Ethical Standards

Conflict of Interest

All authors declared no conflict of interest.


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Copyright information

© Arányi Lajos Foundation 2019

Authors and Affiliations

  • Biao Xie
    • 1
  • Shaolin Nie
    • 2
  • Gui Hu
    • 3
  • Li Xiong
    • 4
  • Fan Hu
    • 1
  • Mei Li
    • 1
  • Tianshu Peng
    • 5
  • Jing Nie
    • 6
  • Yongheng He
    • 1
  1. 1.Department One of Anorectal SurgeryThe Second Affiliated Hospital of Hunan University of Chinese MedicineChangshaPeople’s Republic of China
  2. 2.Department of Colorectal SurgeryHunan Province Tumor HospitalChangshaChina
  3. 3.Department of Gastrointestinal Surgery, The Third Xiangya HospitalCentral South UniversityChangshaChina
  4. 4.Department of Hepatobiliary Surgery, the Second Xiangya HospitalCentral South UniversityChangshaChina
  5. 5.Hunan Slack King Laboratory Animal Co., Ltd.ChangshaChina
  6. 6.Department Four of Anorectal SurgeryThe Second Affiliated Hospital of Hunan University of Chinese MedicineChangshaPeople’s Republic of China

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