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Clinical and Molecular Characterization of Surgically Treated Oropharynx Squamous Cell Carcinoma Samples

  • Ana Carolina de Carvalho
  • Matias Eliseo Melendez
  • Cristina da Silva Sabato
  • Edenir Inez Palmero
  • Lidia Maria Rebolho Batista Arantes
  • Cristovam Scapulatempo Neto
  • André Lopes Carvalho
Original Article
  • 59 Downloads

Abstract

A better understanding of the clinical and molecular features of oropharyngeal squamous cell carcinomas (OPSCC) may help in the development of strategies for a better patient management, improving survival rates. This retrospective study conducted a clinical and molecular characterization of surgically treated OPSCC samples. Paraffin-embedded samples from a series of cases were screened for high-risk (HR) human papillomavirus (HPV) infection, methylation of a 5-gene panel, p53 expression, and TP53 mutation. The study was conducted at Barretos Cancer Hospital. Twenty-five surgically treated OPSCC with available tissue were included in the study. Samples were classified according to HPV status and molecular features and some of these characteristics were associated to clinical data. Twenty percent of the cases were HR-HPV positive and 62.5% presented TP53 mutations. DAPK hypermethylation was associated with HPV status (p = 0.023), while methylated CCNA1 was inversely related to TP53 mutations in primary tumors (p = 0.042) and associated with a better disease-free survival (22.3% vs. 100.0%; p = 0.028) and overall survival (8.0% vs. 100.0%; p = 0.012). The results show differences regarding molecular and clinical characteristics in the oropharynx cases identified that should be validated in more cases to confirm whether these differences are able to classify patients according to outcome and help in a more thorough patient management.

Keywords

Oropharyngeal cancer Human papillomavirus Methylation Mutation Prognosis 

Notes

Acknowledgements

This study was funded by São Paulo Research Foundation (FAPESP), grant # 2015/01286-0 and National Counsel of Technological and Scientific Development (CNPq), grant # 445.053/2014-3. ACC was recipient of scholarship from São Paulo Research Foundation (FAPESP). ALC has a National Counsel of Technological and Scientific Development (CNPq) scholarship.

Supplementary material

12253_2018_462_MOESM1_ESM.doc (34 kb)
Appendix 1 Primers and probes used in the QMSP assays. (DOC 34 kb)
12253_2018_462_MOESM2_ESM.docx (16 kb)
Appendix 2 Description of the clinical and molecular data of the 25 OPSCC patients enrolled in the study. (DOCX 16.3 kb)

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Copyright information

© Arányi Lajos Foundation 2018

Authors and Affiliations

  • Ana Carolina de Carvalho
    • 1
  • Matias Eliseo Melendez
    • 1
  • Cristina da Silva Sabato
    • 3
  • Edenir Inez Palmero
    • 1
    • 3
  • Lidia Maria Rebolho Batista Arantes
    • 1
  • Cristovam Scapulatempo Neto
    • 1
    • 4
  • André Lopes Carvalho
    • 1
    • 2
  1. 1.Molecular Oncology Research CenterBarretos Cancer HospitalBarretosBrazil
  2. 2.Department of Head and Neck SurgeryBarretos Cancer HospitalBarretosBrazil
  3. 3.Center of Molecular DiagnosisBarretosBrazil
  4. 4.Department of PathologyBarretos Cancer HospitalBarretosBrazil

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