A Potential Role for Green Tea as a Radiation Sensitizer for Prostate Cancer
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Prostate cancer (PCa) is the most common non-cutaneous cancer in the United States. There is currently a lack of safe and effective radiosensitizers that can enhance the effectiveness of radiation treatment (RT) for Pca. Clonogenic assay, PCNA staining, Quick Cell Proliferation assay, TUNEL staining and caspase-3 activity assay were used to assess proliferation and apoptosis in DU145 Pca cells. RT-PCR/IHC were used to investigate the mechanisms. We found that the percentage of colonies, PCNA staining intensity, and the optical density value of DU145 cells were decreased (RT/GT vs. RT). TUNEL + cells and the relative caspase-3 activity were increased (RT/GT vs. RT). Compared to RT, the anti-proliferative effect of RT/GT correlated with increased expression of the anti-proliferative molecule p16. Compared to RT, the pro-apoptotic effect of RT/GT correlated with decreased expression of the anti-apoptotic molecule Bcl-2. GT enhances RT sensitivity of DU145 by inhibiting proliferation and promoting apoptosis.
KeywordsGreen tea Prostate cancer Radiation therapy
This study was supported by grants from Des Moines University for Yujiang Fang, MD. PhD. (IOER 05-14-01 and IOER 112-3749). Andrew C. Schroeder, B. S. was supported by Mentored Research Program from Des Moines University (IOER 112-3113).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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