The Limitation of Rapid Tests for DENV2 Infection in Host with Unique Immune Status: Low NS1 Antigenemia and Deficient Antibody Responses

  • Lei YuEmail author
  • Yingfen Wen
  • Mengrong Xiang
  • Wenxin Hong
  • Lingzhai Zhao
  • Fuchun Zhang

Dear Editor,

Dengue infections are caused by all four serotypes of dengue virus (DENV1–4). In recent years it has been confirmed that DENV-1 and DENV-2 are co-circulated in Guangzhou, Guangdong, China (Lai et al. 2015; Luo et al. 2017). DENV-2 strains were imported from Thailand and Indonesia (Luo et al. 2017; Zhao et al. 2014, 2016), and had become the second local circulated serotype in Guangzhou.

Rapid and accurate diagnosis of DENV is crucial for prompt clinical management during an outbreak. The combined NS1 and IgM/IgG tests using immunochromatographic assays (ICs) are recommended for routine diagnosis of dengue fever. But these assays are inherently inferior to ELISA in sensitivity, and thus their usefulness during acute phase of DENV infection is uncertain.

In August 2018, one female (pt3) who had fever and decreased white blood cell count (1.41 × 109/L) was admitted to our hospital. Skin rash was found on her forearms, but thrombocytopenia was not found (platelet count,...



We thank Dr. Yongjun Guan for the consulting of the capture ELISA and the isolation of antibody. This Project was supported by funds from 2017 Guangzhou major projects of collaborative innovation in health care (201704020229), the State Key Laboratory of Pathogen and Biosecurity (SKLPBS1839).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no competing interests.

Informed Consent

The study was approved by the Ethical Committee of the Guangzhou Eighth People’s Hospital (20131224). The samples were collected and used in strict accordance with relevant guidelines and regulations.

Supplementary material

12250_2019_183_MOESM1_ESM.pdf (157 kb)
Supplementary material 1 (PDF 158 kb)


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Copyright information

© Wuhan Institute of Virology, CAS 2020

Authors and Affiliations

  1. 1.Guangzhou Eighth People’s HospitalGuangzhou Medical UniversityGuangzhouChina

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