An Effective Platform for Exploring Rotavirus Receptors by Bacterial Surface Display System

  • Danlei Liu
  • Haoran Geng
  • Zilei Zhang
  • Yifan Xing
  • Danlu Yang
  • Zhicheng Liu
  • Dapeng WangEmail author


Rotavirus (RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens (HBGAs). Previously, the protruding domain protein (P protein) of human norovirus (genotype II.4) was displayed on the surface of Escherichia coli, and it specifically recognized and captured the viral ligands. In order to further verify the feasibility of the system, P protein was replaced by VP8* of RV (G9P[8]) in this study. In the system, VP8* could be correctly released by thrombin treatment with antigenicity retaining, which was confirmed by Western blot and Enzyme-Linked Immunosorbent Assays. Type A HBGAs from porcine gastric mucin (PGM) were recognized and captured by this system. From saliva mixture, the captured viral receptor bound with displayed VP8* was confirmed positive with monoclonal antibody against type A HBGAs. It indicated that the target ligands could be easily separated from the complex matrix. These results demonstrate that the bacterial surface display system will be an effective platform to explore viral receptors/ligands from cell lines or food matrix.


Rotavirus Bacterial surface display system Receptors Histo-blood group antigens (HBGAs) Saliva 



This work was jointly supported by the National Key Research and Development Program of China (2017YFF0210200) and the National Natural Science Foundation of China (31772078).

Author Contributions

DL and DW performed the experiments, DL, HG, ZZ and DW analyzed results and wrote the manuscript. DL, HG, ZZ, YX, DY and DW contributed to experimental design and carried out part of the experiments. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

This article does not contain any studies with human or animal subjects performed by any of the authors.


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Copyright information

© Wuhan Institute of Virology, CAS 2019

Authors and Affiliations

  1. 1.Department of Food Science and Technology, School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghaiChina
  2. 2.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied MicrobiologyGuangdong Institute of MicrobiologyGuangzhouChina
  3. 3.Shanghai Food Safety and Engineering Technology Research Center, School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghaiChina

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