An Effective Platform for Exploring Rotavirus Receptors by Bacterial Surface Display System
Rotavirus (RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens (HBGAs). Previously, the protruding domain protein (P protein) of human norovirus (genotype II.4) was displayed on the surface of Escherichia coli, and it specifically recognized and captured the viral ligands. In order to further verify the feasibility of the system, P protein was replaced by VP8* of RV (G9P) in this study. In the system, VP8* could be correctly released by thrombin treatment with antigenicity retaining, which was confirmed by Western blot and Enzyme-Linked Immunosorbent Assays. Type A HBGAs from porcine gastric mucin (PGM) were recognized and captured by this system. From saliva mixture, the captured viral receptor bound with displayed VP8* was confirmed positive with monoclonal antibody against type A HBGAs. It indicated that the target ligands could be easily separated from the complex matrix. These results demonstrate that the bacterial surface display system will be an effective platform to explore viral receptors/ligands from cell lines or food matrix.
KeywordsRotavirus Bacterial surface display system Receptors Histo-blood group antigens (HBGAs) Saliva
This work was jointly supported by the National Key Research and Development Program of China (2017YFF0210200) and the National Natural Science Foundation of China (31772078).
DL and DW performed the experiments, DL, HG, ZZ and DW analyzed results and wrote the manuscript. DL, HG, ZZ, YX, DY and DW contributed to experimental design and carried out part of the experiments. All authors read and approved the final manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Animal and Human Rights Statement
This article does not contain any studies with human or animal subjects performed by any of the authors.
- Howley P, Knipe D (eds) (2001) Fundamental virology. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
- Lee B, Dickson DM, decamp AC, Colgate ER, Diehl SA, Uddin MI, Sharmin S, Islam S, Bhuiyan TR, Alam M, Nayak U, Mychaleckyj JC, Taniuchi M, Petri WA Jr, Haque R, Qadri F, Kirkpatrick BD (2018) Histo–Blood group antigen phenotype determines susceptibility to genotype-specific Rotavirus infections and impacts measures of Rotavirus vaccine efficacy. J Infect Dis 217:1399–1407CrossRefGoogle Scholar
- Matthijnssens J, Ciarlet M, McDonald S, Attoui H, Bányai K, Brister J, Buesa J, Esona M, Estes M, Gentsch J, Iturriza-Gómara M, Johne R, Kirkwood C, Martella V, Mertens P, Nakagomi O, Parreño V, Rahman M, Ruggeri F, Saif L, Santos N, Steyer A, Taniguchi K, Patton J, Desselberger U, Van Ranst M (2011) Uniformity of rotavirus strain nomenclature proposed by the rotavirus classification working group (RCWG). Adv Virol 156:1397–1413Google Scholar
- Nordgren J, Sharma S, Bucardo F, Nasir W, Günaydın G, Ouermi D, Nitiema LW, Becker-Dreps S, Simpore J, Hammarström L, Larson G, Svensson L (2014) Both Lewis and secretor status mediate susceptibility to rotavirus infections in a rotavirus genotype-dependent manner. Clin Infect Dis 59:1567–1573CrossRefGoogle Scholar
- Troeger C, Khalil I, Rao P, Cao S, Blacker B, Ahmed T, Armah G, Bines J, Brewer T, Colombara D, Kang G, Kirkpatrick B, Kirkwood C, Mwenda J, Parashar U, Petri W, Riddle M, Steele A, Thompson R, Walson J, Sanders J, Mokdad A, Murray CJ, Hay S, Reiner R (2018) Rotavirus vaccination and the global burden of rotavirus diarrhea among children younger than 5 years. JAMA Pediatr 172:958–965CrossRefGoogle Scholar