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Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection

  • Jiadai Li
  • Lingling Han
  • Yao Hao
  • Yuncong Yuan
  • Mingzhen Wang
  • Xiu Xin
  • Hailong Wang
  • Fang Yu
  • Congyi Zheng
  • Chao ShenEmail author
RESEARCH ARTICLE

Abstract

Foot-and-mouth disease virus (FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome- and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1 (Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication.

Keywords

Foot-and-mouth disease virus (FMDV) Persistent infection RNA-Seq Differentially expressed gene (DEG) 

Notes

Acknowledgements

We appreciate the discussion with Dr. Xun Liu (Huazhong University of Science and Technology). We acknowledge the financial support of the National Infrastructure of Natural Resources for Science and Technology Program (No.2011-572), the National Natural Sciences Foundation of China (No. 31370185), the National Basic Research Program of China (No. 2011CB504800) to Prof. Congyi Zheng, and the Fundamental Research Funds for the Central Universities (2042018kf0241) and the National Science and Technology Infrastructure Grants (NSTI-CR15 and NSTI-CR16) to Dr. Chao Shen.

Author Contributions

JL, LH, CZ and CS contributed to experimental design; JL and YY performed the experiments; JL and MW contributed to data analysis; JL, CZ, YH and CS contributed to data interpretation; JL wrote the manuscript; XX, HW, FY, CS and CZ checked and finalized the manuscript. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

This article does not contain any studies with human or animal subjects performed by any of the authors.

Supplementary material

12250_2019_155_MOESM1_ESM.pdf (73 kb)
Supplementary material 1 (PDF 73 kb)
12250_2019_155_MOESM2_ESM.xlsx (881 kb)
Supplementary material 2 (XLSX 881 kb)

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Copyright information

© Wuhan Institute of Virology, CAS 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Virology, College of Life SciencesWuhan UniversityWuhanChina
  2. 2.Department of Pathology, Zhongnan HospitalWuhan UniversityWuhanChina
  3. 3.China Center for Type Culture CollectionWuhan UniversityWuhanChina

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