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Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan

  • Altaf Hussain
  • Tiantian Wu
  • Hui Li
  • Linjin Fan
  • Kai Li
  • Li Gao
  • Yongqiang Wang
  • Yulong Gao
  • Changjun Liu
  • Hongyu Cui
  • Qing Pan
  • Yanping Zhang
  • Asim Aslam
  • Khan Muti-Ur-Rehman
  • Muhammad Munir
  • Salman Latif Butt
  • Xiaomei WangEmail author
  • Xiaole QiEmail author
Letter
  • 11 Downloads

Dear Editor,

Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a non-enveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan, poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector. However, there is limited genome information of IBDV available in Pakistan (Shabbir et al.2016).

In March 2017, a suspected IBD outbreak was reported in 28-day-old broiler chicken flock in Punjab, which is the most important chicken-farming area of Pakistan. A total of 30% to 50% morbidity and 13% mortality were observed without previous history of the vaccination. Seventeen (n = 17) bursa samples were collected for virus detection. Using RT-PCR with primers of A628U/A1540L (Supplementary Table S1), a specific fragment of IBDV...

Notes

Acknowledgements

This work was supported by the National Key Research and Development Program of China (Nos. 2016YFE0203200, 2017YFD0500704), the Major Project of National Natural Science Foundation of China (No. 31430087), the Modern Agro-industry Technology Research System (No. CARS-41-G15).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

Animal experiments in this study were approved by the Ethics Committees of Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences (CAAS) (Approval Number: SQ-2017-080).

Supplementary material

12250_2019_82_MOESM1_ESM.pdf (109 kb)
Supplementary material 1 (PDF 109 kb)

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Copyright information

© Wuhan Institute of Virology, CAS 2019

Authors and Affiliations

  1. 1.Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research InstituteThe Chinese Academy of Agricultural SciencesHarbinChina
  2. 2.OIE Reference Laboratory for Infectious Bursal DiseaseHarbinChina
  3. 3.Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Disease and ZoonosesYangzhouChina
  4. 4.Pathology DepartmentUniversity of Veterinary and Animal SciencesLahorePakistan
  5. 5.Division of Biomedical and Life Sciences, Faculty of Health and MedicineLancaster UniversityLancasterUK
  6. 6.Department of Pathology, College of Veterinary MedicineUniversity of GeorgiaAtlantaUSA

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