Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan
- 11 Downloads
Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a non-enveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan, poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector. However, there is limited genome information of IBDV available in Pakistan (Shabbir et al.2016).
In March 2017, a suspected IBD outbreak was reported in 28-day-old broiler chicken flock in Punjab, which is the most important chicken-farming area of Pakistan. A total of 30% to 50% morbidity and 13% mortality were observed without previous history of the vaccination. Seventeen (n = 17) bursa samples were collected for virus detection. Using RT-PCR with primers of A628U/A1540L (Supplementary Table S1), a specific fragment of IBDV...
This work was supported by the National Key Research and Development Program of China (Nos. 2016YFE0203200, 2017YFD0500704), the Major Project of National Natural Science Foundation of China (No. 31430087), the Modern Agro-industry Technology Research System (No. CARS-41-G15).
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Animal and Human Rights Statement
Animal experiments in this study were approved by the Ethics Committees of Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences (CAAS) (Approval Number: SQ-2017-080).
- Hernández M, Tomás G, Marandino A, Iraola G, Maya L, Mattion N, Hernández D, Villegas P, Banda A, Panzera Y (2015) Genetic characterization of south american infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage. Avian Pathol 44:212–221CrossRefGoogle Scholar
- Qi X, Gao L, Qin L, Deng X, Wu G, Zhang L, Yu F, Ren X, Gao Y, Gao H (2011) Genomic sequencing and molecular characteristics of a very virulent strain of infectious bursal disease virus isolated in china. Agr Sci Technol 12:1946–1949Google Scholar