American Journal of Potato Research

, Volume 96, Issue 5, pp 447–456 | Cite as

Ammonium Gluconate Production from Potato Starch Wastewater Using a Multi-Enzyme Process

  • Bingcui Chen
  • Kang Li
  • Lingtong Liao
  • Ruiming Wang
  • Piwu LiEmail author


In China, potato starch wastewater causes serious environmental pollution. Therefore, in this study, we aimed to develop a method for the transformation of potato starch in potato starch wastewater into ammonium gluconate, where potato starch wastewater was enzymatically hydrolyzed by liquefaction and saccharification. The optimum parameters of the hydrolysis process were as follows: α-amylase concentration, 0.5% (v/v); reaction time, 35 min; temperature, 90 °C; glucoamylase concentration, 0.7% (v/v); reaction time, 25 h; temperature, 50 °C; and pH, 4.0. Subsequently, ammonium gluconate was produced using 4.6 U/g glucose oxidase and 75 U/g catalase at 50 °C. The pH of the solution was maintained in the range of 6.8 ± 0.2. In the final crude ammonium gluconate-containing liquid, the protein, total nitrogen, total carbon, organic carbon, and ammonium salt content was 3.5 g/L, 8.47 g/L, 53.62 g/L, 13.79 g/ L, and 60 g/L, respectively. The environmental pollution was solved by using potato starch to produce value-added products.


Environmental pollution Hydrolysis Liquefaction Potato starch Saccharification 



Dextrose equivalent


Potato starch wastewater


Total suspended solid


En China, el agua residual del almidón de papa causa serios problemas de contaminación. De aquí que, en este estudio, nos propusimos desarrollar un método para la transformación del agua residual del almidón de la papa en gluconato de amonio, donde el agua fue hidrolizada enzimáticamente por licuefacción y sacarificación. Los parámetros óptimos del proceso de hidrólisis fueron los siguientes: concentración de α-amilasa, 0.5% (v/v); tiempo de reacción, 35 min; temperatura, 90 °C; concentración de glucoamilasa, 0.7% (v/v); tiempo de reacción, 25 h; temperatura, 50 °C; y pH, 4.0. Subsecuentemente, se produjo el gluconato de amonio usando 4.6 U/g de glucosa oxidasa y 75 U/g catalasa a 50 °C. El pH de la solución se mantuvo en el rango de 6.8 ± 0.2. En el líquido final crudo que contenía el gluconato de amonio, el contenido de la proteína, nitrógeno total, carbón total, carbón orgánico, y la sal de amonio fue de 3.5 g/L, 8.47 g/L, 53.62 g/L, 13.79 g/ L, y 60 g/L, respectivamente. Se resolvió la contaminación ambiental mediante el uso del almidón de la papa para producir productos con valor agregado.



This work was supported by the Shandong Provincial Natural Science Foundation (ZR2016CB04), Major Program of National Natural Science Foundation of Shandong (ZR2017ZB0208), National Science Foundation of China (31801527, C200201), Major Science and Technology Projects in Shandong Province (2016CYJS07A01).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12230_2019_9733_MOESM1_ESM.docx (440 kb)
ESM 1 (DOCX 440 kb)


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Copyright information

© The Potato Association of America 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Bio-based material & Green Papermaking, School of BioengineeringQilu University of Technology (Shandong Academy of Sciences)JinanRepublic of China

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