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Germplasm with Resistance to Potato virus Y Derived from Solanum chacoense: Clones M19 (39–7) and M20 (XD3)

  • Ana Cristina Fulladolsa
  • Amy Charkowski
  • Xingkui Cai
  • Jonathan Whitworth
  • Stewart Gray
  • Shelley JanskyEmail author
Article
  • 34 Downloads

Abstract

Potato virus Y (PVY) causes one of the most serious and widespread diseases in North America. In recent years, the virus has become increasingly difficult to control. Durable dominant genes for resistance to PVY exist in potato germplasm and provide an effective control strategy. This paper describes a Solanum chacoense clone (M19) that is homozygous for a PVY resistance gene. The gene is linked to a previously published marker for Rychc found in Solanum chacoense. M19 was crossed with a diploid S. tuberosum clone to produce an adapted clone carrying the resistance gene. This hybrid clone is named M20. M20 tuberizes in the field, producing round tubers with white skin and flesh and moderate size. M20 is resistant to PVYO, PVYN:O, and PVYNTN. Both M19 and M20 are female and male fertile, so they are being released as sources of PVY resistance for breeding programs.

Keywords

PVY Wild potato Crop wild relative Breeding Disease resistance 

Resumen

El Virus Y de la Papa (PVY) causa una de las enfermedades más serias y de amplia distribución en Norteamérica. En años recientes, el virus ha aumentado la dificultad para su control. Los genes durables dominantes para resistencia al PVY existen en el germoplasma de papa y proporcionan una estrategia de control efectivo. Este artículo describe un clon (M19) de Solanum chacoense que es homozigótico para un gen de resistencia al PVY. El gen esta ligado a un marcador previamente publicado para Rychc encontrado en Solanum chacoense. Se cruzó M19 con un clon diploide de S. tuberosum para producir un clon adaptado llevando el gen de resistencia. A este clon híbrido se le llama M20. Este clon tuberiza en el campo, produciendo tubérculos redondos con piel y pulpa blancas, de tamaño moderado. M20 es resistente al PVYO, PVYN:O y PVYNTN. Ambos M19 y M20 son fértiles como hembra y macho, de manera que están siendo liberados como fuentes de Resistencia al PVY para los programas de mejoramiento.

Notes

Acknowledgements

This work is supported by SCRI grant number 73999-10921 from USDA National Institute of Food and Agriculture. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The authors thank Drs. Douches and Perkins for their assistance with glycoalkaloid analysis.

References

  1. Asama, K., H. Ito, N. Murakami, and T. Itoh. 1982. New potato variety “Konafubuki”. Bull Hokkaido Pref Agr Exp Station 48: 75–84.Google Scholar
  2. Berger, P.H., M.J. Adams, O.W. Barnett, A.A. Brunt, J. Hammond, et al. 2005. Family potyviridae. In Virus taxonomy: Classification and nomenclature of viruses. 8th report of the international committee on taxonomy of viruses, ed. C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, and L.A. Ball, 819–841. London: Elsevier Acad.Google Scholar
  3. Cai, X., D. Spooner, and S. Jansky. 2011. A test of taxonomic and biogeographic predictivity: Resistance to Potato virus Y in wild relatives of the cultivated potato. Phytopathology 101: 1074–1080.CrossRefPubMedGoogle Scholar
  4. Clark, M.F., and A.N. Adams. 1977. Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34: 475–483.CrossRefPubMedGoogle Scholar
  5. Cockerham, G. 1943. Potato breeding for virus resistance. Annals of Applied Biology 30: 105–108.CrossRefGoogle Scholar
  6. De Jong, H., and P.R. Rowe. 1971. Inbreeding in cultivated diploid potatoes. Potato Research 14: 74–83.CrossRefGoogle Scholar
  7. Flis, B., J. Hennig, D. Strzelczyk-Zyta, C. Gebhardt, and W. Marczewski. 2005. The Ry-fsto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to chromosome XII and is diagnosed by PCR marker GP122 718 in PVY resistant potato cultivars. Molecular Breeding 15: 95–101.CrossRefGoogle Scholar
  8. Fulladolsa, A.C., F.M. Navarro, R. Kota, K. Severson, J.P. Palta, and A.O. Charkowski. 2015. Application of marker assisted selection for Potato virus Y resistance in the University of Wisconsin Potato Breeding Program. American Journal of Potato Research 92: 444–450.CrossRefGoogle Scholar
  9. Fulladolsa, A. C., Jansky, S. H., Smith, D. R., Abramczak, C. M., & Charkowski, A. O. 2017. Development and evaluation of four molecular markers tightly linked to the Potato virus Y resistance gene Ry chc in diploid potato populations. American Phytopathological Society 258-P.Google Scholar
  10. German, T.L. 2001. Potato virus Y. In Compendium of potato diseases, ed. W.R. Stevenson, R. Loria, G.D. Franc, and D.P. Weingartner, 2nd ed., 69–71. Minnesota: The American Phytopathological Society Press.Google Scholar
  11. Gray, S., S. De Boer, J. Lorenzen, A. Karasev, J. Whitworth, P. Nolte, R. Singh, A. Boucher, and H. Xu. 2010. Potato virus Y: An evolving concern for potato crops in the United States and Canada. Plant Disease 94: 1384–1397.CrossRefPubMedGoogle Scholar
  12. Hamalainen, J.H., K.N. Watanabe, J.P. Valkonen, A. Arihara, R.L. Plaisted, E. Pehu, L. Miller, and S.A. Slack. 1997. Mapping and marker-assisted selection for a gene for extreme resistance to Potato virus Y. Theoretical and Applied Genetics 94: 192–197.CrossRefGoogle Scholar
  13. Hane, D.C., and P.B. Hamm. 1999. Effects of seedborne Potato virus Y infection in two potato cultivars expressing mild disease symptoms. Plant Disease 83: 43–45.CrossRefPubMedGoogle Scholar
  14. Hosaka, K., Y. Hosaka, M. Mori, T. Maida, and H. Matsunaga. 2001. Detection of a simplex RAPD marker linked to resistance to Potato virus Y in a tetraploid potato. American Journal of Potato Research 78: 191–196.CrossRefGoogle Scholar
  15. Karasev, A.V., and S.M. Gray. 2013. Continuous and emerging challenges of Potato virus Y in potato. Annual Review of Phytopathology 51: 571–586.CrossRefPubMedGoogle Scholar
  16. Kopp, A., M. Kondrák, and Z. Bánfalvi. 2015. Review article: Molecular mechanisms of resistance to potato virus X and Y in potato. Acta Phytopathologica et Entomologica Hungarica 50: 151–160.CrossRefGoogle Scholar
  17. MacKenzie, T.D.B., J. Lavoie, X. Nie, and M. Singh. 2018. Differential spread of Potato virus Y (PVY) strains O, N:O and NTN in the field: Implications for the rise of recombinant PVY strains in New Brunswick, Canada. American Journal of Potato Research 95: 301–310.CrossRefGoogle Scholar
  18. Mori, K., Y. Sakamoto, N. Mukojima, S. Tamiya, T. Nakao, T. Ishii, and K. Hosaka. 2011. Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato. Euphytica 180: 347–355.CrossRefGoogle Scholar
  19. Mori, K., N. Mukojima, T. Nakao, S. Tamiy, Y. Sakamoto, N. Sohbaru, K. Hayashi, H. Watanuki, K. Nara, K. Yamazaki, T. Ishii, and K. Hosaka. 2012. Germplasm release: Saikai 35, a male and female fertile breeding line carrying Solanum phureja-derived cytoplasm and potato cyst nematode resistance (H1) and potato virus Y resistance (Ry chc) genes. American Journal of Potato Research 89: 63–72.CrossRefGoogle Scholar
  20. Munoz, R.J., R.L. Plaisted, and H.D. Thurston. 1975. Resistance to Potato virus Y in Solanum tuberosum ssp. andigena. American Potato Journal 52: 107–115.CrossRefGoogle Scholar
  21. Murakami, N., H. Matsunaga, K. Senda, Y. Okuyama, M. Iritani, K. Asama, Y. Mitsui, and K. Shimizu. 1995. A new potato variety “Konamuso” (=Sakaruafubuki)". Bull Hokkaido Pref Agr Exp Station 68: 1–16.Google Scholar
  22. Nolte, P., J.L. Whitworth, M.K. Thornton, and C.S. McIntosh. 2004. Effect of seedborne Potato virus Y on performance of Russet Burbank, Russet Norkotah, and Shepody potato. Plant Disease 88: 248–252.CrossRefPubMedGoogle Scholar
  23. Ottoman, R.J., D. Hane, C. Brown, S. Yilma, A. Mosley, and M.I. Vales. 2007. Usefulness of molecular markers to screen for PVY resistance (Ry adg gene) in potato. American Journal of Potato Research 84: 108.Google Scholar
  24. Quenouille, J., N. Vassilakos, and B. Moury. 2013. Potato virus Y: A major crop pathogen that has provided major insights into the evolution of viral pathogenicity. Molecular Plant Pathology 14: 439–452.CrossRefPubMedGoogle Scholar
  25. Sato, M., K. Nishikawa, K. Komura, and K. Hosaka. 2006. Potato virus Y resistance gene, Ry chc, mapped to the distal end of potato chromosome 9. Euphytica 149: 367–372.CrossRefGoogle Scholar
  26. Scholthof, K.-B.G., S. Adkins, H. Czosnek, P. Palukaitis, E. Jacquot, T. Hohn, B. Hohn, K. Saunders, T. Candresse, P. Ahlquist, C. Hemenway, and G.D. Foster. 2011. Top 10 plant viruses in molecular plant pathology. Molecular Plant Pathology 12: 938–954.CrossRefPubMedGoogle Scholar
  27. Song, Y.-S., and A. Schwarzfischer. 2008. Development of STS markers for selection of extreme resistance (Ry sto) to PVY and maternal pedigree analysis of extremely resistant cultivars. American Journal of Potato Research 85: 159–170.CrossRefGoogle Scholar
  28. Song, Y.-S., L. Hepting, G. Schweizer, L. Hartl, G. Wenzel, and A. Schwarzfischer. 2005. Mapping of extreme resistance to PVY (Ry sto) on chromosome XII using anther-culture-derived primary dihaploid potato lines. Theoretical and Applied Genetics 111: 879–887.CrossRefPubMedGoogle Scholar
  29. Vales, M.I., R.J. Ottoman, J.A. Ortega, S. Yilma, and E. Karaagac. 2010. Marker-assisted selection for PVY resistance in tetraploid potatoes. Acta Horticulturae 859: 409–416.CrossRefGoogle Scholar
  30. Whitworth, J.L., R.G. Novy, D.G. Hall, J.M. Crosslin, and C.R. Brown. 2009. Characterization of broad spectrum Potato Virus Y resistance in a Solanum tuberosum ssp. andigena -derived population and select breeding clones using molecular markers, grafting, and field inoculations. American Journal of Potato Research 86: 286–296.CrossRefGoogle Scholar

Copyright information

© The Potato Association of America 2019

Authors and Affiliations

  1. 1.Department of Bioagricultural Sciences and Pest ManagementColorado State UniversityFort CollinsUSA
  2. 2.College of Horticulture & Forestry SciencesHuazhong Agricultural UniversityWuhanChina
  3. 3.USDA-ARSAberdeenUSA
  4. 4.USDA-ARS and Section of Plant Pathology & Plant-Microbe Biology, School of Integrated Plant ScienceCornell UniversityIthacaUSA
  5. 5.USDA-ARS and Department of HorticultureUniversity of WisconsinMadisonUSA

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