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American Journal of Potato Research

, Volume 96, Issue 1, pp 1–5 | Cite as

Three Cycles of Recurrent Maternal Half-Sib Selection Continue to Reduce Foliar Late Blight in a Diploid Hybrid Solanum phureja-S. stenotomum Population

  • Kathleen G. HaynesEmail author
  • Xinshun Qu
Article
  • 64 Downloads

Abstract

Increasing levels of foliar resistance to late blight, caused by Phytophthora infestans, have previously been reported through two cycles of recurrent maternal half-sib selection in a diploid hybrid population of Solanum phureja-S. stenotomum. The objective of this study was to determine if continued improvements for resistance to late blight could be realized by another cycle of selection. Four clones from each of 72 maternal half-sib families constituting the cycle four population were evaluated for late blight resistance in replicated field trials in Pennsylvania in 2015 and 2016. The experimental design was a randomized complete block design with two replications each year. P. infestans US23 genotype was used in inoculations each year. Area under the disease progress curve (AUDPC) was calculated based on visual assessment of foliar disease five times late in the season each year and subjected to statistical and genetic analyses. Mean AUDPC in the cycle one, two, three, and four populations was 652, 556, 276, and 173, respectively. Narrow-sense heritability for resistance decreased in the cycle four population (h2 = 0.54) as compared to prior populations (0.77 ≤ h2 ≤ 0.80), however, broad-sense heritability remained high (H = 0.83). Additional improvements for late blight resistance are likely to occur if this approach is continued.

Keywords

Diploid potato breeding Germplasm enhancement Phytophthora infestans Narrow-sense heritability Broad-sense heritability Population improvement 

Resumen

Se ha reportado previamente aumento en los niveles de resistencia foliar al tizón tardío, causado por Phytophthora infestans, a lo largo de dos ciclos de selección maternal recurrente media en una población de híbrido diploide de Solanum phureja-S. stenotomum. El objetivo de este estudio fue determinar si mejoramientos continuos para resistencia al tizón tardío pudieran hacerse mediante otro ciclo de selección. Cuatro clones de cada una de 72 medias familias maternales que constituyeron la población del cuarto ciclo se evaluaron para resistencia al tizón tardío en ensayos de campo repetidos en Pennsylvania en 2015 y 2016. El diseño experimental fue de bloques completos al azar con dos repeticiones por año. Se usó el genotipo US23 de P. infestans en las inoculaciones de cada año. Se calculó el área bajo la curva de progreso de la enfermedad (AUDPC) con base en evaluación foliar de la enfermedad cinco veces al final del ciclo cada año y fue objeto de análisis estadístico y genético. La media del AUDPC en las poblaciones de los ciclos uno, dos, tres y cuatro fue de 652, 556, 276 y 173, respectivamente. La heredabilidad para la resistencia en el sentido estrecho disminuyó en la población del cuarto ciclo (h2 = 0.54) en comparación con las poblaciones previas (0.77 < h2 < 0.80). No obstante, la heredabilidad en el sentido amplio permaneció alta (H = 0.83). Es probable que se presenten mejoramientos adicionales para la resistencia al tizón tardío si se continúa con este enfoque.

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Copyright information

© The Potato Association of America 2018

Authors and Affiliations

  1. 1.Agricultural Research Service, Genetic Improvement of Fruits and Vegetables Laboratory, U.S. Department of AgricultureBeltsvilleUSA
  2. 2.Department of Plant Pathology and Environmental MicrobiologyThe Pennsylvania State UniversityUniversity ParkUSA

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