American Journal of Potato Research

, Volume 95, Issue 2, pp 170–177 | Cite as

An Evaluation of two H1-Linked Markers and their Suitability for Selecting Globodera rostochiensis Resistant Potatoes in the New York Breeding Program

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Abstract

The golden cyst nematode (Globodera rostochiensis) is a serious pest that can dramatically reduce potato crop yield. Pathotype Ro1 of G. rostochiensis was first detected in the United States in 1941 and is still present on several farms in New York State. The H1 gene confers high levels of resistance to pathotype Ro1 but screening for it with a bioassay is time consuming and expensive. In this study two known molecular markers, 57R and TG689, were evaluated for their ability to identify resistant clones among 38 global cultivars and 350 New York breeding clones. The ability of either marker to predict resistance was high – 99.7% and 98.3% for 57R and TG689, respectively – but the ability to predict susceptibility was much lower, 47% and 41%, respectively. As resistance is the trait of interest, either of these markers is sufficient to make selection decisions in a practical breeding program. Cases exhibiting discordance between presence/absence of diagnostic markers and bioassay results were investigated further. Recombination, inflow of other resistance genes, and occasional failure of marker- and/or bio-assays are discussed as potential causes.

Keywords

Potato cyst nematode Nematode resistant cultivar Bioassay Predictive value Molecular markers 

Resumen

El nematodo dorado (Globodera rostochiensis) es una plaga seria que puede reducir dramáticamente el rendimiento del cultivo de papa. El patotipo Ro1 de G. rostochiensis fue detectado por primera vez en los Estado Unidos en 1941 y aún está presente en varios ranchos en el Estado de Nueva York. El gen H1 confiere altos niveles de resistencia al patotipo Ro1, pero evaluarlo con un bioensayo lleva tiempo y es caro. En este estudio, dos marcadores moleculares, 57R y TG689, se evaluaron para su habilidad de identificar clones resistentes entre 38 variedades globales y 350 clones avanzados de Nueva York. La habilidad de cualquier marcador para predecir la resistencia fue alta – 99.7% y 98.3% para 57R y TG689, respectivamente – pero la habilidad para predecir la susceptibilidad fue mucho más baja, 47% y 41%, respectivamente. Como la resistencia es el carácter de interés, cualquiera de estos marcadores es suficiente para tomar decisiones de selección en un programa práctico de mejoramiento. Se investigaron más adelante los casos que presentaron discordancia entre presencia/ausencia de marcadores de diagnóstico y resultados de bioesayos. Se discuten la recombinación, la entrada de otros genes de resistencia y la falla ocasional del marcador y/o los bioensayos, como causas potenciales.

Notes

Acknowledgements

We thank David Thurston for assistance with nematode bioassays and Shuping Cheng for some PCR tests. This work was supported in part by the USDA ARS-State Partnership Potato Program (award 58-1907-4-024), a USDA ARS specific cooperative agreement (Development and Release of Golden Nematode Resistant Potatoes; award 58-8062-5-033) and USDA NIFA (award 2015-69004-23634).

Supplementary material

12230_2017_9623_MOESM1_ESM.xlsx (20 kb)
ESM 1 (XLSX 20 kb)

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Copyright information

© The Potato Association of America 2017

Authors and Affiliations

  1. 1.School of Integrative Plant ScienceCornell UniversityIthacaUSA
  2. 2.Robert W. Holley Center for Agriculture and Health, US Department of Agriculture, Agricultural Research ServiceIthacaUSA

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