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Folia Geobotanica

, Volume 53, Issue 4, pp 429–447 | Cite as

Genome size variation in the genus Andryala (Hieraciinae, Asteraceae)

  • Jaroslav ZahradníčekEmail author
  • Jindřich Chrtek
  • Maria Zita Ferreira
  • Anna Krahulcová
  • Judith Fehrer
Article

Abstract

The genus Andryala includes diploid plants distributed in the Mediterranean Basin, Macaronesia and in one isolated outpost in the Romanian Carpathians. We analysed nuclear genome size in a phylogenetic framework and assessed relationships between genome size and life form (perennials vs annuals/biennials) and the consequences of insular vs continental distribution. Absolute nuclear genome size of 18 species or subspecies was determined using propidium iodide flow cytometry. The evolution of genome size was investigated using the Brownian motion model with the tree scaling parameters λ, κ and δ. The mean 2C values differs up to 1.84-fold between species (from 2.69 to 5.01 pg). Chromosome numbers of six species are reported for the first time. The highest 2C values are present in two well supported basal lineages corresponding to the relict species A. laevitomentosa and A. agardhii. The rest of Andryala species that form a well-supported clade of closely related species (the ‘Major Radiation Group’), except for two populations of A. ragusina, have significantly smaller genome sizes. In the ‘Major Radiation Group’, genome size in perennial species is significantly greater compared to annual and biennial species. With a possible bias caused by A. dentata and A. integrifolia, insular species of the ‘Major Radiation Group’ have lower nuclear genomes than continental ones. The genome size variation contains strong phylogenetic signal, which could indicate that genome size in the group under study is not greatly influenced by selection and is probably a result of neutral evolution or genetic drift.

Keywords

Andryala Asteraceae Genome size Macaronesia Mediterranean Basin Phylogeny 

Notes

Acknowledgements

We are grateful to I. Álvarez, M. Benedito, E. Bräutigam, S. Bräutigam, Z. Dočkalová, I. Ferreira, R. Jardim, K. Krak, L. Medina, P. Mráz, V. Mrázová, A. Pupo Correia, M. Puskás, M. Menezes de Sequeira, T. Urfus, K. Vazačová, P. Vít, J. Vítová, P. Zahradníčková and A. Zeddam for their collaboration with field sampling and plant collecting. Fred Rooks kindly improved our English. This work was supported by the Czech Science Foundation (P506/10/1353 to J.Ch.) and the long-term research development project No. RVO 67985939 of the Czech Academy of Sciences.

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Copyright information

© Institute of Botany, Academy of Sciences of the Czech Republic 2018

Authors and Affiliations

  • Jaroslav Zahradníček
    • 1
    Email author
  • Jindřich Chrtek
    • 1
    • 2
  • Maria Zita Ferreira
    • 3
  • Anna Krahulcová
    • 2
  • Judith Fehrer
    • 2
  1. 1.Department of Botany, Faculty of ScienceCharles University in PraguePragueCzech Republic
  2. 2.Institute of Botany of the Czech Academy of SciencesPrůhoniceCzech Republic
  3. 3.Faculdade de Ciências da VidaUniversidade da MadeiraFunchalPortugal

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