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Multicellular growth of the Basidiomycota phytopathogen fungus Sporisorium reilianum induced by acid conditions

  • Domingo Martínez-SotoEmail author
  • John Martin Velez-Haro
  • Claudia Geraldine León-Ramírez
  • Edgardo Galán-Vásquez
  • Bibiana Chávez-Munguía
  • José Ruiz-Herrera
Original Article
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Abstract

Fungi are considered model organisms for the analysis of important phenomena of eukaryotes. For example, some of them have been described as models to understand the phenomenon of multicellularity acquisition by different unicellular organisms phylogenetically distant. Interestingly, in this work, we describe the multicellular development in the model fungus S. reilianum. We observed that Sporisorium reilianum, a Basidiomycota cereal pathogen that at neutral pH grows with a yeast-like morphology during its saprophytic haploid stage, when incubated at acid pH grew in the form of multicellular clusters. The multicellularity observed in S. reilianum was of clonal type, where buds of “stem” cells growing as yeasts remain joined by their cell wall septa, after cytokinesis. The elaboration and analysis of a regulatory network of S. reilianum showed that the putative zinc finger transcription factor CBQ73544.1 regulates a number of genes involved in cell cycle, cellular division, signal transduction pathways, and biogenesis of cell wall. Interestingly, homologous of these genes have been found to be regulated during Saccharomyces cerevisiae multicellular growth. In adddition, some of these genes were found to be negatively regulated during multicellularity of S. reilianum. With these data, we suggest that S. reilianum is an interesting model for the study of multicellular development.

Keywords

Sporisorium reilianum Multicellular growth Acid conditions Fungal development 
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Notes

Acknowledgments

Thanks are given to Prof. Jan Schirawski (Institute of Applied Microbiology, RWTH Aachen University, Germany), for making available the S. reilianum strains. We also thank Lizbeth Salazar-Villatoro (CINVESTAV I.P.N., Zacatenco Unit), Marco A. Mancilla-Avila (Universidad Autónoma de San Luis Potosí), and Mayela F. Salazar-Chávez (CINVESTAV, Irapuato Unit), for assistance in some analyses. This work was partially supported by Consejo Nacional de Ciencia y Tecnología (CONACYT), México

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interests.

Supplementary material

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2019

Authors and Affiliations

  • Domingo Martínez-Soto
    • 1
    • 2
    • 3
    • 4
    Email author
  • John Martin Velez-Haro
    • 2
    • 5
  • Claudia Geraldine León-Ramírez
    • 2
  • Edgardo Galán-Vásquez
    • 2
  • Bibiana Chávez-Munguía
    • 6
  • José Ruiz-Herrera
    • 2
  1. 1.Ingeniería en Innovación Agrícola SustentableInstituto Tecnológico Superior de Los ReyesLos ReyesMéxico
  2. 2.Departamento de Ingeniería Genética, Unidad IrapuatoCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalGuanajuatoMéxico
  3. 3.Department of Plant Pathology and MicrobiologyUniversity of CaliforniaRiversideUSA
  4. 4.Ingeniería en Innovación Agrícola SustentableInstituto Tecnológico Superior de Los ReyesLibertadMéxico
  5. 5.Departamento de Ingeniería BioquímicaInstituto Tecnológico de CelayaGuanajuatoMéxico
  6. 6.Departamento de Infectómica y Patogénesis MolecularCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalCd. de MéxicoMéxico

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