Folia Microbiologica

, Volume 64, Issue 6, pp 821–834 | Cite as

Characterization of a broad spectrum bacteriocin produced by Lactobacillus plantarum MXG-68 from Inner Mongolia traditional fermented koumiss

  • Li-Li Man
  • Dian-Jun XiangEmail author
Original Article


An agar well diffusion assay (AWDA) was used to isolate a high bacteriocin-producing strain with a broad spectrum of antibacterial activity, strain MXG-68, from Inner Mongolia traditional fermented koumiss. Lactobacillus plantarum MXG-68 was identified by morphological, biochemical, and physiological characteristics and 16S rDNA analysis. The production of antibacterial substance followed a growth-interrelated model, starting at the late lag phase of 4 h and arriving at a maximum value in the middle of the stationary phase at 24 h. Antibacterial activity was abolished or decreased in the presence of pepsin, chymotrypsin, trypsin, proteinase, and papain K. The results showed that antibacterial substances produced by L. plantarum MXG-68 were proteinaceous and could thus be classified as the bacteriocin, named plantaricin MXG-68. The molar mass of plantaricin MXG-68 was estimated to be 6.5 kDa, and the amino acid sequence of its N-terminal was determined to be VYGPAGIFNT. The mode of plantaricin MXG-68 action was determined to be bactericidal. Bacteriocin in cell-free supernatant (CFS) at pH 7 was stable at different temperatures (60 °C, 80 °C, 100 °C, 121 °C for 30 min; 4 °C and − 20 °C for 30 days), as well as at pH 2.0–10.0. Antibacterial activity maintained stable after treatment with organic solvents, surfactants, and detergents but increased in response to EDTA. Response surface methodology (RSM) revealed the optimum conditions of bacteriocin production in L. plantarum MXG-68, and the bacteriocin production in medium optimized by RSM was 26.10% higher than that in the basal MRS medium.


Bacteriocin Screening Lactobacillus plantarum Characterization Response surface methodology 


Author contributions

Li-Li Man and Dian-Jun Xiang contributed equally to this article. Li-Li Man and Dian-Jun Xiang performed the experiments and contributed significantly to the data analysis, results discussion and manuscript preparation.

Funding information

This study was supported by the Doctoral Research Start-up Fund of Inner Mongolia University for Nationalities (No. BS403), Natural Sciences Foundation of Inner Mongolia Autonomous Region of China (No. 2018MS03060).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics statement

No animals or humans were used in this study.

Open access

This article is distributed under the terms of the Creative Commons At tribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2019

Authors and Affiliations

  1. 1.College of Life ScienceInner Mongolia University for NationalitiesTongliaoPeople’s Republic of China
  2. 2.College of AgricultureInner Mongolia University for NationalitiesTongliaoPeople’s Republic of China

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