Bci528I, a new isoschizomer of EcoRI isolated from Bacillus circulans 528
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Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Bci528I recognizes 5′-GAATTC-3′ in dsDNA and cleaves between G and A of the recognition sequence, producing a symmetric four base 5′overhang.
We thank Dr. Chol-Min Pak of the research institute of biotechnology, academy of science, the DPR of Korea for determination of recognition sequence and cleavage site.
Sung-Ryong Ra conceived and designed the experiments. Myong-Suk Kim and Chon-Il Paek conducted experiments with support from Hyong-Bom Pak, Kum-Chol Ri, and Yong-Chol Pak. Song Hui Pak analyzed the data. Chon-Il Paek wrote the manuscript. All authors read and approved the final manuscript.
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