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Folia Microbiologica

, Volume 64, Issue 4, pp 471–479 | Cite as

Introducing a thermotolerant Gluconobacter japonicus strain, potentially useful for coenzyme Q10 production

  • Foozieh Moghadami
  • Jamshid FooladiEmail author
  • Ramin Hosseini
Original Article
  • 126 Downloads

Abstract

In this report, Gluconobacter strains were screened for coenzyme Q10 (CoQ10) production. A thermotolerant strain, Gluconobacter japonicus FM10, was eventually employed for CoQ10 production optimization. To do so, a two-step optimization strategy was used. The first step focused on biomass increase and the second step focused on increase in CoQ10 production. Factors including temperature, pH, carbon, and nitrogen sources were optimized at the first step, and temperature, pH, and aeration were optimized at the second step. The batch culture fermentation was used with the optimized factors of the first phase (30 °C, pH 6.5, D-sorbitol, and yeast extract-peptone as the carbon and nitrogen sources). After 18 h, the temperature, pH, and aeration were shifted to the optimized values of the second step (36 °C, pH 7, and no aeration). By this strategy, the dry cell mass (17.1 g/L) and CoQ10 (23.2 mg/L) were obtained after 20 h, which the latter was 2.3 times higher than that of the first step of optimization. Among the conditions tested, carbon source was the most important factor on the cell growth at the first step while no aeration was the key factor for CoQ10 production in the second step of optimization.

Keywords

Coenzyme Q10 Gluconobacter Thermotolerant Optimization 

Notes

Acknowledgments

F. Moghadami gives her special gratitude to Prof. Osao Adachi, Yamaguchi University, Japan, for his encouragements and the inspiration he gave her to work on acetic acid bacteria.

Funding information

The authors would like to gratefully acknowledge the financial support from Research Council of Alzahra University.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

12223_2018_666_MOESM1_ESM.docx (84 kb)
ESM 1 (DOCX 83 kb)

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2019

Authors and Affiliations

  • Foozieh Moghadami
    • 1
  • Jamshid Fooladi
    • 2
    Email author
  • Ramin Hosseini
    • 3
  1. 1.Department of Microbiology, Faculty of Biological SciencesAlzahra UniversityTehranIran
  2. 2.Department of Biotechnology, Faculty of Biological SciencesAlzahra UniversityTehranIran
  3. 3.Department of Biotechnology, Faculty of Agriculture and Natural ResourcesImam Khomeini International UniversityQazvinIran

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