Correct species identification (reclassification in CNCTC) of strains of Staphylococcus intermedius-group can improve an insight into their evolutionary history
- 90 Downloads
A group of 59 putative strains of Staphylococcus intermedius/Staphylococcus pseudintermedius deposited in the Czech National Collection of Type Cultures (CNCTC, National Institute for Public Health, Prague, Czech Republic) and the National Reference Laboratory for Staphylococci (NRL for Staphylococci, National Institute for Public Health, Prague, Czech Republic) was reclassified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There the biggest human collection of S. pseudintermedius in Europe was analysed; 44 samples (75%) were of human origin. Twenty-two percent (n = 13) of the strains were isolated from animals, and two staphylococci were of unknown origin. This study revealed the prevalence of Staphylococcus pseudintermedius (94%, n = 53) vs. Staphylococcus intermedius (6%, n = 6) in the collection of human and veterinary staphylococci after reclassification. Results of PCR-RFLP analysis were verified by comparison with a repetitive element sequence-based polymerase chain reaction (Rep-PCR) analysis on 26 (44%) randomly selected strains. Due to a low-resolution ability of PCR-RFLP to separate Staphylococcus intermedius from Staphylococcus delphini, four isolates of Staphylococcus intermedius were biochemically verified further to exclude the presence of Staphylococcus delphini in the collection. Our results indicate that S. intermedius and S. pseudintermedius have occurred independently over an age-long period of their co-evolution.
The authors thank Pavel Švec, Ph.D., from the Czech Collection of Microorganisms (Brno, Czech Republic) for Rep-PCR analysis of staphylococci.
This project was supported by MH CZ-DRO (“The National Institute of Public Health-NIPH, 75010330”) and SVV 260398.
- Bannoehr J, Ben Zakour NL, Waller AS, Guardabassi L, Thoday KL, van den Broek AHM, Fitzgerald JR (2007) Population genetic structure of the Staphylococcus intermedius group: insights into agr diversification and the emergence of methicillin-resistant strains. J Bacteriol 189:8685–8692. https://doi.org/10.1128/JB.01150-07 CrossRefGoogle Scholar
- Jorgensen JH, Pfaller MA, Carrroll k LML, Funke G, Richter S, Warnock DW (2015) Manual of clinical microbiology. ASM Press, WashingtonGoogle Scholar
- Kmieciak W, Szewczyk EM (2018) Are zoonotic Staphylococcus pseudintermedius strains a growing threat for humans? Folia Microbiol (Praha)Google Scholar
- Murugaiyan J, Walther B, Stamm I, Abou-Elnaga Y, Brueggemann-Schwarze S, Vincze S, Wieler LH, Lübke-Becker A, Semmler T, Roesler U (2014) Species differentiation within the Staphylococcus intermedius group using a refined MALDI-TOF MS database. Clin Microbiol Infect 20:1007–1014CrossRefGoogle Scholar
- Olive DM, Bean P (1999) Principles and applications of methods for DNA-based typing of microbial organisms. J Clin Microbiol 37:1661–1669Google Scholar
- Ventrella G, Moodley A, Grandolfo E, Parisi A, Corrente M, Buonavoglia D, Guardabassi L (2017) Frequency, antimicrobial susceptibility and clonal distribution of methicillin-resistant Staphylococcus pseudintermedius in canine clinical samples submitted to a veterinary diagnostic laboratory in Italy: a 3-year retrospective investigation. Vet Microbiol 211:103–106CrossRefGoogle Scholar