Folia Microbiologica

, Volume 64, Issue 2, pp 231–236 | Cite as

Correct species identification (reclassification in CNCTC) of strains of Staphylococcus intermedius-group can improve an insight into their evolutionary history

  • Lucia MališováEmail author
  • Renáta Šafránková
  • Jana Kekláková
  • Petr Petráš
  • Helena Žemličková
  • Vladislav Jakubů
Original Article


A group of 59 putative strains of Staphylococcus intermedius/Staphylococcus pseudintermedius deposited in the Czech National Collection of Type Cultures (CNCTC, National Institute for Public Health, Prague, Czech Republic) and the National Reference Laboratory for Staphylococci (NRL for Staphylococci, National Institute for Public Health, Prague, Czech Republic) was reclassified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There the biggest human collection of S. pseudintermedius in Europe was analysed; 44 samples (75%) were of human origin. Twenty-two percent (n = 13) of the strains were isolated from animals, and two staphylococci were of unknown origin. This study revealed the prevalence of Staphylococcus pseudintermedius (94%, n = 53) vs. Staphylococcus intermedius (6%, n = 6) in the collection of human and veterinary staphylococci after reclassification. Results of PCR-RFLP analysis were verified by comparison with a repetitive element sequence-based polymerase chain reaction (Rep-PCR) analysis on 26 (44%) randomly selected strains. Due to a low-resolution ability of PCR-RFLP to separate Staphylococcus intermedius from Staphylococcus delphini, four isolates of Staphylococcus intermedius were biochemically verified further to exclude the presence of Staphylococcus delphini in the collection. Our results indicate that S. intermedius and S. pseudintermedius have occurred independently over an age-long period of their co-evolution.



The authors thank Pavel Švec, Ph.D., from the Czech Collection of Microorganisms (Brno, Czech Republic) for Rep-PCR analysis of staphylococci.

Funding information

This project was supported by MH CZ-DRO (“The National Institute of Public Health-NIPH, 75010330”) and SVV 260398.


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2018

Authors and Affiliations

  1. 1.National Reference Laboratory for AntibioticsNational Institute of Public HealthPragueCzech Republic
  2. 2.Czech National Collection of Type CulturesNational Institute of Public HealthPragueCzech Republic
  3. 3.Faculty of Medicine in Hradec KrálovéCharles UniversityHradec KrálovéCzech Republic
  4. 4.National Reference Laboratory for StaphylococciNational Institute of Public HealthPragueCzech Republic

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