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Folia Microbiologica

, Volume 64, Issue 2, pp 189–195 | Cite as

Comparative restriction enzyme mapping of Campylobacter jejuni isolates from turkeys and broilers based on flaA flagellar gene using HpyF3I endonuclease

  • Elham Atefi Tabar
  • Hamid StajiEmail author
  • Ali Mahdavi
Original Article
  • 62 Downloads

Abstract

Turkeys and broilers have been identified as important reservoirs for Campylobacter jejuni which is of public health significance. The evaluation of the genotypes among C. jejuni strains within different reservoirs is critical for our understanding of the epidemiology of this infectious agent. The present study aimed to compare the genetic diversity and differences of C. jejuni isolates from turkeys and broilers using flagellin PCR-RFLP typing (flaA typing) technique, in terms of the ease of use and discriminatory power. Sixty C. jejuni isolates were detected biochemically and confirmed by duplex-PCR from turkeys and broilers (30 strains from each bird species). Then, a flaA gene fragment (1725 bp) of C. jejuni isolates was amplified and amplicons were digested with HpyF3I enzyme. Restriction analysis by HpyF3I gave four different flaA patterns (H1, H2, H3, H4) among all tested C. jejuni isolates. In broiler isolates, all four patterns were observed but in turkey isolates, only H2 and H4 patterns were present. The results clearly demonstrated that distribution of the flaA typing patterns differed depending on the host species (broiler/turkey). H1 and H3 flaA types are more prevalent in broiler than turkey isolates, while H2 type is significantly more prevalent within isolates from turkey (p < 0.05). The flaA typing technique by digestion with HpyF3I enzyme can almost give us a clue to the source of infection in local outbreaks.

Notes

Acknowledgments

The authors gratefully acknowledge the staff of laboratory of microbiology, faculty of veterinary medicine, University of Semnan (Iran), especially Mrs. Behnaz Raeisian and Mrs. Birgani Farhani. This study was carried out in partial fulfillment of the requirements for a DVM student’s thesis (Elham Atefi Tabar). We deeply thank Dr. Masoumeh Estaji (Allameh Tabataba’i University) for English revision of the manuscript.

Funding information

We acknowledge Semnan University for funding the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2018

Authors and Affiliations

  1. 1.Faculty of Veterinary MedicineSemnan UniversitySemnanIran
  2. 2.Department of Pathobiology, Faculty of Veterinary MedicineSemnan UniversitySemnanIran
  3. 3.Department of Animal Sciences, Faculty of Veterinary MedicineSemnan UniversitySemnanIran

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