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Folia Microbiologica

, Volume 64, Issue 2, pp 171–175 | Cite as

Echinosporin antibiotics isolated from Amycolatopsis strain and their antifungal activity against root-rot pathogens of the Panax notoginseng

  • Xindong Xu
  • Li HanEmail author
  • Lixing Zhao
  • Xiao Chen
  • Cuiping Miao
  • Linfang Hu
  • Xueshi Huang
  • Youwei Chen
  • Yiqing LiEmail author
Original Article

Abstract

Actinomycete strain YIM PH20520, isolated from the rhizosphere soil sample of Panax notoginseng collected in Wenshang, Yunnan Province, China, exhibited antifungal activity against root-rot pathogens of the Panax notoginseng. The structures of bioactive molecules, isolated from the ethyl acetate extract of the fermentation broth of the strain, were identified as echinosporin (1) and 7-deoxyechinosporin (2) based on extensive spectroscopic analyses. 1 exhibited antifungal activity against four tested root-rot pathogens of Panax notoginseng include Fusarium oxysporum, Fusarium solani, Alternaria panax, and Phoma herbarum with the MIC value at 64, 64, 32, and 64 μg/mL, respectively. 2 exhibited antifungal activities against F. oxysporum, F. solani, A. panax, and P. herbarum with the MIC value at 128, 128, 64, and 128 μg/mL, respectively. Based on the phylogenetic analyses, the closest phylogenetic relative of strain YIM PH20520 is Amycolatopsis speibonae JS72T (97.69%), so strain YIM PH20520 was identified as Amycolatopsis strain. To the best of our knowledge, this is the first report of echinosporin antibiotics isolated from Amycolatopsis strain besides Streptomyces strain and their antifungal activity against four tested root-rot pathogens of the Panax notoginseng. The results provide a reliable evidence for the following related biosynthetic investigations on Amycolatopsis strain YIM PH20520 due to echinosporins antibiotics’ unique tricyclic acetal-lactone structures.

Notes

Funding information

This work was supported by the National Natural Science Foundation of China (Grant No. 31660532, 21562045, 31660004).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Statement of human rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Statement on the welfare of animals

This article does not contain any studies with human participants or vertebrate animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

12223_2018_642_MOESM1_ESM.pdf (247 kb)
ESM 1 (PDF 246 kb)

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2018

Authors and Affiliations

  • Xindong Xu
    • 1
  • Li Han
    • 2
    Email author
  • Lixing Zhao
    • 1
  • Xiao Chen
    • 1
  • Cuiping Miao
    • 1
  • Linfang Hu
    • 1
  • Xueshi Huang
    • 2
  • Youwei Chen
    • 1
  • Yiqing Li
    • 1
    Email author
  1. 1.Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, College of Life SciencesYunnan UniversityKunmingChina
  2. 2.Institute of Microbial Pharmaceuticals, College of Life and Health SciencesNortheastern UniversityShenyangChina

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