Analysis of genetic variations of heat shock proteins Hsp70 and Hsp90 in Isaria farinosa strains from the Yunnan province of China
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In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populations distributed in the Yunnan province. The complete sequence of the IFHSP70 cDNA had a length of 2158 bp, and contained an open reading frame (ORF) of 1962 bp, encoding a 71-kDa polypeptide comprising of 653 amino acids. IFHSP90 cDNA had a length of 2144 bp, and contained an ORF of 2103 bp, encoding a polypeptide of 79.23 kDa, comprising of 700 amino acids. The deduced amino acid sequences of IFHSP70 and IFHSP90 shared high sequence identities with other fungi. Fundamental information pertaining to the protein families, signatures, and conserved motifs of Hsp70 and Hsp90 were also identified. Analysis of molecular variances (AMOVA) from the Hsp70 and Hsp90 genes showed that the genetic variation within-population (83.26%, 83.08%) was greater than among the populations (16.74%, 16.92%). The values of nucleotide diversity (Pi), haplotype diversity (Hd), coefficient of genetic differentiation (Fst), and gene flow (Nm) were calculated. For Hsp70, Pi = 0.0425, Hd = 0.888, Fst = 0.167, Nm = 1.24; For Hsp90, Pi = 0.0420, Hd = 0.894, Fst = 0.169, and Nm = 1.22. These data indicated that the genetic differentiation among 10 different geographical populations of I. farinosa was limited. This study describes, for the first time, cloning, characterization and identification of Isaria farinosa Hsp70 and Hsp90 genes, and provides a preliminary basis for exploring the genetic structure of the genus Isaria using the sequences of Hsp70 and Hsp90 as molecular markers.
This work was supported by the National Natural Science Foundation of China (31760011) and the China Postdoctoral Science Foundation (2017 M613017).
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Conflict of interest
The authors declare that they have no conflict of interest.
- Akaboot P, Duangjinda M, Phasuk Y, Kaenchan C, Chinchiyanond W (2012) Genetic characterization of Red Jungle fowl (Gallus gallus), Thai indigenous chicken (Gallus domesticus), and two commercial lines using selective functional genes compared to microsatellite markers. Genet Mol Res 11:1881–1890CrossRefPubMedGoogle Scholar
- Allendorf FW (1983) Gene flow and genetic differentiation among populations. Genet Conserv 18:51–65Google Scholar
- Barua D, Heckathorn SA, Coleman JS (2008) Variation in heat-shock proteins and photosynthetic thermotolerance among natural populations of Chenopodium album L. from contrasting thermal environments: implications for plant responses to global warming. J Integr Plant Biol 50:1440–1451CrossRefPubMedGoogle Scholar
- Clements FE, Shear CL (1931) The genera of Fungi. Hafner Press, New York, p 407Google Scholar
- Gallou A, Serna-Domínguez MG, Berlanga-Padilla AM, Ayala-Zermeño MA, Mellín-Rosas MA, Montesinos-Matías R, Arredondo-Bernal HC (2015) Species clarification of Isaria isolates used as biocontrol agents against Diaphorina citri (Hemiptera: Liviidae) in Mexico. Fungal Biol 120:1–10Google Scholar
- Kobayasi Y (1941) The genus Cordyceps and its allies. Science Report of the Tokyo Bunrika Daigaku 5:53–260Google Scholar
- Samon RA (1974) Paecilomyces and some allied Hyphomycetes. Stud Mycol 6:1–119Google Scholar
- Tiwari S, Shankar J (2018) Hsp70 in fungi: evolution, function and vaccine candidate. In: Asea A, Kaur P (eds) Hsp70 in human diseases and disorders. Heat shock proteins, vol 14. Springer, ChamGoogle Scholar
- Wright S (1979) Evolution and the genetics of population. University of Chicago Press, ChicagoGoogle Scholar