Tandem Biocatalysis by CotA-TJ102@UIO-66-NH2 and Novozym 435 for Highly Selective Transformation of HMF into FDCA

  • Xin Chang
  • Chengyu Zhang
  • Lan Gao
  • Xiao Liu
  • Shengping YouEmail author
  • Wei QiEmail author
  • Kang Wang
  • Xin Guo
  • Rongxin Su
  • Han Lu
  • Zhimin He
Research Article


2,5-Furandicarboxylic acid (FDCA) is a potential biorenewable chemical for applications including plastics, polyamides, drugs, etc. The selective biosynthesis of FDCA from 5-hydroxymethylfurfural (HMF) by a specific enzyme poses a great challenge. In this study, we reported an efficient strategy to produce FDCA from HMF by the tandem biocatalysis of laccase (CotA-TJ102@UIO-66-NH2) and Novozym 435. For the first step, a nanoparticle metal–organic framework was synthesized as a carrier to immobilize CotA-TJ102@UIO-66-NH2, which was assigned for the production of 5-formyl-2-furancarboxylic acid (FFCA) and featured an enzyme loading of 255.54 mg/g, specific activity of 135.90 U/mg, and solid loading ratio of 99.65%. Under optimal conditions, an ideal FFCA yield of 98.5% was achieved, and the CotA-TJ102@UIO-66-NH2 presented a high recycling capacity after 10 cycles. For the second step, Novozym 435 was applied for the further conversion of FFCA into FDCA, presenting a high FDCA yield of 95.5% under the optimized conditions. Novozym 435 also exhibited a high recyclability after eight cycles. As a result, the tandem biocatalysis strategy provided a 94.2% FDCA yield from HMF, indicating its excellence as a method for FDCA production.


CotA-TJ102@UIO-66-NH2 Novozym 435 Tandem biocatalysis 5-Hydroxymethylfurfural 2, 5-Furandicarboxylic acid 



This work was supported by the National Key R&D Program of China (No. 2017YFB0306502), the Science Fund for Creative Research Groups (No. 21621004), the Project funded by China Postdoctoral Science Foundation (2019), the Key Project of Tianjin Science and Technology Committee (No. 17YFZCSY01080), and the Program of Beiyang Young Scholar of Tianjin University (2012).

Supplementary material

12209_2019_215_MOESM1_ESM.docx (1.4 mb)
Supplementary material 1 (DOCX 1429 kb)


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Copyright information

© Tianjin University and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Xin Chang
    • 1
  • Chengyu Zhang
    • 1
  • Lan Gao
    • 2
  • Xiao Liu
    • 1
  • Shengping You
    • 1
    • 3
    • 4
    Email author
  • Wei Qi
    • 1
    • 3
    • 4
    • 5
    Email author
  • Kang Wang
    • 2
  • Xin Guo
    • 2
  • Rongxin Su
    • 1
    • 3
    • 4
    • 5
  • Han Lu
    • 2
  • Zhimin He
    • 1
    • 3
  1. 1.Chemical Engineering Research Center, School of Chemical Engineering and TechnologyTianjin UniversityTianjinChina
  2. 2.Research Institute of Petroleum Processing, SINOPECBeijingChina
  3. 3.State Key Laboratory of Chemical EngineeringTianjin UniversityTianjinChina
  4. 4.Tianjin Key Laboratory of Membrane Science and Desalination TechnologyTianjin UniversityTianjinChina
  5. 5.Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)TianjinChina

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