Transactions of Tianjin University

, Volume 25, Issue 1, pp 38–44 | Cite as

Isolation of Secondary Metabolites with Antimicrobial Activities from Bacillus amyloliquefaciens LWYZ003

  • Zhen Liu
  • Yiting Wang
  • Xiaoqiang Jia
  • Wenyu LuEmail author
Research Article


The strain LWYZ003, which can restrain multiple pathogens, was screened from the sediment of the ocean and identified as Bacillus amyloliquefaciens. Large-scale fermentation and modern chromatographic separation technologies (macroporous resin column chromatography, silica gel column chromatography, thin-layer chromatography and high-performance liquid chromatography) were used to separate antimicrobial products from the fermentation broth of marine-derived Bacillus amyloliquefaciens LWYZ003. Bioactive-guided separation was used in the term of seeking antimicrobial products from the secondary metabolites of Bacillus amyloliquefaciens LWYZ003. As a result, two natural products cycloheximide (1) and trehalose (2) were obtained. Their structures were elucidated by Fourier transform infrared spectroscopy, high-resolution mass spectrometry, 1H and 13C nuclear magnetic resonance analysis. In the cylinder plate method, compound 1 exhibited stronger antimicrobial activities than compound 2 against Micrococcus luteus, and also exhibited wider antimicrobial spectrum than compound 2. In conclusion, isolation of bioactive secondary metabolites from marine Bacillus sp. has enormous potentials in finding suitable antibiotics to inhibit multiple pathogens.


Cycloheximide Trehalose Bacillus amyloliquefaciens Bioactivity-guided isolation Antibiotic 



This work was supported by the National Basic Research Program of China ("973" Program) (no. 2012CB721105) and the Major Research Plan of Tianjin, China (no. 16YFXTSF00460).

Supplementary material

12209_2018_137_MOESM1_ESM.doc (6.9 mb)
Supplementary material 1 (DOC 7020 kb)


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Copyright information

© Tianjin University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Zhen Liu
    • 1
    • 2
    • 3
  • Yiting Wang
    • 1
    • 2
    • 3
  • Xiaoqiang Jia
    • 1
    • 2
    • 3
  • Wenyu Lu
    • 1
    • 2
    • 3
    Email author
  1. 1.School of Chemical Engineering and TechnologyTianjin UniversityTianjinChina
  2. 2.Key Laboratory of System BioengineeringMinistry of EducationTianjinChina
  3. 3.SynBio Research PlatformCollaborative Innovation Center of Chemical Science and EngineeringTianjinChina

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