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Journal of Mechanical Science and Technology

, Volume 33, Issue 11, pp 5571–5580 | Cite as

Effects of the cell and triangular microwell size on the cell-trapping efficacy and specificity

  • Tewan Tongmanee
  • Werayut Srituravanich
  • Achariya Sailasuta
  • Witsaroot Sripumkhai
  • Wutthinan Jeamsaksiri
  • Kenichi Morimoto
  • Yuji Suzuki
  • Alongkorn PimpinEmail author
Article
  • 6 Downloads

Abstract

The single-, double- or multiple-cell entrapment efficacy is crucial in various aspects of biological studies. In this study, both computational and experimental approaches were conducted to explore the effect of the cell and microwell sizes on the ability of cell-trapping in a triangular microwell. From computational studies, it was found that the interaction between a spanwise vortex on the upper part and counter-rotating streamwise vortices at the leading edge helped to form a pair of secondary streamwise vortices deeper inside the microwell. The strength and size of these secondary streamwise vortices, which depended on the size of the microwell, played an important role in the arrangement of entrapped cells. The experimental results, with both microbeads and white blood cells (WBCs), were in good agreement with the simulated ones, and suggested that the ratio between the cell and microwell sizes was an important factor in the efficacy of single-, double- and multiple-cell cell-trapping. Entrapment of canine WBCs (size distribution between 7–15 µm) attained a highest single-cell trapping efficiency of 20.4 % in the array of 40-µm triangular microwells of 30 µm depth at a flow rate of 0.1 mL/h, but this was reduced to 16.5 and 10.6 % in the 60- and 80-µm microwells, respectively, under the same conditions.

Keywords

Cell trapping Triangular microwell Microfluidics Flow circulation White blood cells 

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Notes

Acknowledgments

Financial support was received through Chulalongkorn Academic Advancement into its 2nd Century Project (Smart Medical Device & 3D Printing for Product Development). T.T. was a Graduate Research Fellow (The 90th Anniversary of Chulalongkorn University Fund). The authors would like to thank Dr. Mayuree Chanasakulniyom and Dr. Dettachai Ketpun for their helpful assistance. Dr. Takayuki Nakagawa (The University of Tokyo) provided cell line in this study.

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Copyright information

© KSME & Springer 2019

Authors and Affiliations

  • Tewan Tongmanee
    • 1
  • Werayut Srituravanich
    • 1
  • Achariya Sailasuta
    • 2
  • Witsaroot Sripumkhai
    • 3
  • Wutthinan Jeamsaksiri
    • 3
  • Kenichi Morimoto
    • 4
  • Yuji Suzuki
    • 4
  • Alongkorn Pimpin
    • 1
    Email author
  1. 1.Department of Mechanical Engineering, Faculty of EngineeringChulalongkorn UniversityBangkokThailand
  2. 2.Department of Pathology, Faculty of Veterinary ScienceChulalongkorn UniversityBangkokThailand
  3. 3.Thai Microelectronics Center (TMEC)ChachoengsaoThailand
  4. 4.Department of Mechanical EngineeringThe University of TokyoTokyoJapan

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