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Journal of Mechanical Science and Technology

, Volume 33, Issue 4, pp 1841–1850 | Cite as

Bioreactor mimicking knee-joint movement for the regeneration of tissue-engineered cartilage

  • Hun-Jin Jeong
  • So-Jung Gwak
  • Nae-Un Kang
  • Myoung Wha Hong
  • Young Yul Kim
  • Young-Sam ChoEmail author
  • Seung-Jae LeeEmail author
Article
  • 3 Downloads

Abstract

Efforts to minimize the sacrifice of laboratory animals have become a recent worldwide trend. This trend has triggered a number of studies toward developing effective methods to replace the animal experiments. In this study, we developed a biomimetic bioreactor system that simulates the movements of the human knee joint. The system consists of a knee-joint drive and a unit capable of culturing cells at the joint surface. The knee-joint drive is designed to apply dynamic stimulation similar to the real bending motion of the knee joint. We employed a commercial incubator for comparative evaluation and validation of our laboratory-made cell-culture unit mounted in a bioreactor. The results revealed that the ability of the proposed system in culturing cells was similar to that of the commercial incubator. The cell culture was evaluated by dividing the knee joint into zones according to the size of the stimulus. The results confirmed that the cell assessment stimulated by the knee-joint movement was two times higher than that having no stimulation. Overall, the study helped establish that the cell characteristics is more effective when an appropriate external stimulus is applied according to the target tissue. The study is also expected to form the basis for implementing an ex-vivo environment that can potentially replace animal and cadaver experiments in the future. In the future, follow-up studies will be conducted on the in-vivo environment and the characteristics of each organ and tissue for effective tissue regeneration.

Keywords

Cartilage tissue engineering CPM (continuous passive motion) Ex-vivo system Knee joint SLUP (salt leaching using powder) 

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References

  1. [1]
    Z. Chunqiu, Z. Xizheng, W. Han, H. Daqing and G. Jing, Direct compression as an appropriately mechanical environment in bone tissue reconstruction in vitro, Med. Hypotheses, 67(6) (2006) 1414–1418.CrossRefGoogle Scholar
  2. [2]
    P. Reher, N. I. Elbeshir, W. Harvey, S. Meghji and M. Harris, The stimulation of bone formation in vitro by therapeutic ultrasound, Ultrasound Med. Biol., 23(8) (1997) 1251–1258.CrossRefGoogle Scholar
  3. [3]
    G. Vunjak-Novakovic, L. Meinel, G. Altman and D. Kaplan, Bioreactor cultivation of osteochondral grafts, Orthod Craniofac Res, 8(3) Aug (2005) 209–218.CrossRefGoogle Scholar
  4. [4]
    Z. Y. Zhang et al., A biaxial rotating bioreactor for the culture of fetal mesenchymal stem cells for bone tissue engineering, Biomaterials, 30(14) May (2009) 2694–2704.CrossRefGoogle Scholar
  5. [5]
    J. P. Vacanti and R. Langer, Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation, Lancet, 354(1) Jul. (1999) SI32–34.CrossRefGoogle Scholar
  6. [6]
    G. Jin, G. H. Yang and G. Kim, Tissue engineering bioreactor systems for applying physical and electrical stimulations to cells, J. Biomed. Mater. Res. B Appl. Biomater, 103(4) May (2015) 935–948.CrossRefGoogle Scholar
  7. [7]
    H.-J. P. N.-K. Lee and K.-M. Lim, Reconstructed human skin and cornea models as alternative methods to animal tests, Journal of Alternatives to Animal Experiments, 8 (2014) 29–36.Google Scholar
  8. [8]
    G. Vunjak-Novakovic, K. O. Lui, N. Tandon and K. R. Chien, Bioengineering heart muscle: A paradigm for regenerative medicine, Annu. Rev. Biomed. Eng., 13 (2011) 245–267.CrossRefGoogle Scholar
  9. [9]
    H. T. Au, I. Cheng, M. F. Chowdhury and M. Radisic, Interactive effects of surface topography and pulsatile electrical field stimulation on orientation and elongation of fibroblasts and cardiomyocytes, Biomaterials, 28(29) Oct. (2007) 4277–4293.CrossRefGoogle Scholar
  10. [10]
    L. M. G. Vunjak-Novakovic, G. Altman and D. Kaplan, Bioreactor cultivationof osteochondral grafts, Orthidintics & Craniofacial Res., 8(3) (2005) 209–218.CrossRefGoogle Scholar
  11. [11]
    D. Wendt, A. Marsano, M. Jakob, M. Heberer and I. Martin, Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity (in English), Biotechnology and Bioengineering, 84(2) (2003) 205–214.CrossRefGoogle Scholar
  12. [12]
    D. W. S. Scaglione, S. Miggino, A. Papadimitropoulos, M. Fato, R. Quarto and I. Martin, Effects of fluid flow and calcium phosphate coating on human bone marrow stromal cells cultured in a defined 2D model system, Journal of Biomedical Materials Research Part A, 86 (2007) 411–419.Google Scholar
  13. [13]
    M. C. Qi, J. Hu, S. J. Zou, H. Q. Chen, H. X. Zhou and L. C. Han, Mechanical strain induces osteogenic differentiation: Cbfa1 and Ets-1 expression in stretched rat mesenchymal stem cells (in English), International Journal of Oral and Maxillofacial Surgery, 37(5) May (2008) 453–458.CrossRefGoogle Scholar
  14. [14]
    D. Kaspar, W. Seidl, C. Neidlinger-Wilke, A. Beck, L. Claes and A. Ignatius, Proliferation of human-derived osteoblast-like cells depends on the cycle number and frequency of uniaxial strain (in English), Journal of Biomechanics, 35(7) Jul. (2002) 873–880.CrossRefGoogle Scholar
  15. [15]
    C. Y. C. Huang, K. L. Hagar, L. E. Frost, Y. B. Sun and H. S. Cheung, Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells (in English), Stem Cells, 22(3) (2004) 313–323.CrossRefGoogle Scholar
  16. [16]
    O. Demarteau et al., Dynamic compression of cartilage constructs engineered from expanded human articular chondrocytes (in English), Biochemical and Biophysical Research Communications, 310(2), Oct. 17 (2003) 580–588.CrossRefGoogle Scholar
  17. [17]
    P. F. Davies and S. C. Tripathi, Mechanical stress mechanisms and the cell. An endothelial paradigm, Circ. Res., 72(2) Feb. (1993) 239–245.CrossRefGoogle Scholar
  18. [18]
    P. Angele et al., Cyclic hydrostatic pressure enhances the chondrogenic phenotype of human mesenchymal progenitor cells differentiated in vitro (in English), Journal of Orthopaedic Research, 21(3) May (2003) 451–457.CrossRefGoogle Scholar
  19. [19]
    K. Shahin and P. M. Doran, Tissue engineering of cartilage using a mechanobioreactor exerting simultaneous mechanical shear and compression to simulate the rolling action of articular joints (in English), Biotechnology and Bioengineering, 109(4) Apr. (2012) 1060–1073.CrossRefGoogle Scholar
  20. [20]
    G. E. Nugent-Derfus et al., Continuous passive motion applied to whole joints stimulates chondrocyte biosynthesis of PRG4, Osteoarthritis Cartilage, 15(5) May (2007) 566–574.CrossRefGoogle Scholar
  21. [21]
    Y. S. Cho, B. S. Kim, H. K. You and Y. S. Cho, A novel technique for scaffold fabrication: SLUP (salt leaching using powder) (in English), Current Applied Physics, 14(3) Mar. (2014) 371–377.CrossRefGoogle Scholar
  22. [22]
    B. M. Tymrak, M. Kreiger and J. M. Pearce, Mechanical properties of components fabricated with open-source 3-D printers under realistic environmental conditions (in English), Materials & Design, 58 (2014) 242–246.CrossRefGoogle Scholar
  23. [23]
    D. K. M. I. D. Johnston, C. K. L. Tan and M. C. Tracey, Mechanical characterization of bulk Sylgard 184 for microfluidics and microengineering, Journal of Micromechanics and Microengineering, 24 (2014) 7.CrossRefGoogle Scholar
  24. [24]
    J. Yang et al., Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture (in English), Journal of Biomedical Materials Research, 62(3) (2002) 438–446.CrossRefGoogle Scholar
  25. [25]
    J. H. P. I. H. Lee, S.-J. Lee, D.-W. Cho and S. S. Kan, Effects of mechanical stimulation for MC3T3-E1 cells using bioreactor, The Korean Society of Mechanical Engineers, 11 (2008) 1411–1414.Google Scholar

Copyright information

© KSME & Springer 2019

Authors and Affiliations

  • Hun-Jin Jeong
    • 1
  • So-Jung Gwak
    • 2
  • Nae-Un Kang
    • 1
  • Myoung Wha Hong
    • 3
  • Young Yul Kim
    • 3
  • Young-Sam Cho
    • 1
    • 4
    Email author
  • Seung-Jae Lee
    • 1
    • 4
    Email author
  1. 1.Department of Mechanical EngineeringWonkwang UniversityJeonbukKorea
  2. 2.Department of Chemical EngineeringWonkwang UniversityJeonbukKorea
  3. 3.Department of Orthopedics, Daejeon St. Mary’s HospitalCatholic University of KoreaDaejeonKorea
  4. 4.Department of Mechanical Design EngineeringWonkwang UniversityJeonbukKorea

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