Research on Spatially Adaptive High-Order Total Variation Model for Weak Fluorescence Image Restoration

  • Jin Ma (马进)Email author
  • Teng Xue (薛腾)
  • Quanquan Shao (邵全全)
  • Jie Hu (胡洁)
  • Weiming Wang (王伟明)


Confocal laser scanning microscopy (CLSM) has emerged as one of the most advanced fluorescence cell imaging techniques in the field of biomedicine. However, fluorescence cell imaging is limited by spatial blur and additive white noise induced by the excitation light. In this paper, a spatially adaptive high-order total variation (SA-HOTV) model for weak fluorescence image restoration is proposed to conduct image restoration. The method consists of two steps: optimizing the deconvolution model of the fluorescence image by the generalized Lagrange equation and alternating direction method of multipliers (ADMM); using spatially adaptive parameters to balance the image fidelity and the staircase effect. Finally, an comparison of SA-HOTV model and Richardson-Lucy model with total variation (RL-TV model) indicates that the proposed method can preserve the image details ultimately, reduce the staircase effect substantially and further upgrade the quality of the restored weak fluorescence image.

Key words

confocal microscopy weak fluorescence image restoration spatially adaptive high-order total variation (SA-HOTV) 

CLC number

TP 391.4 

Document code


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Copyright information

© Shanghai Jiaotong University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jin Ma (马进)
    • 1
    Email author
  • Teng Xue (薛腾)
    • 1
  • Quanquan Shao (邵全全)
    • 1
  • Jie Hu (胡洁)
    • 1
  • Weiming Wang (王伟明)
    • 1
  1. 1.State Key Laboratory of Mechanical System and VibrationShanghai Jiao Tong UniversityShanghaiChina

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