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Cellular and Molecular Bioengineering

, Volume 12, Issue 1, pp 99–106 | Cite as

Ultrasound Enhanced Anti-tumor Effect of Temozolomide in Glioblastoma Cells and Glioblastoma Mouse Model

  • Jie Xue
  • Yuanyuan Wu
  • Na LiuEmail author
Article

Abstract

Introduction

Glioblastoma is the most aggressive cancer that begins within the brain. In clinic, temozolomide was used as anti-tumor drugs for glioblastoma chemotherapy, but showed limited effect. Therefore, how to improve the effect of temozolomide to glioblastoma is urgently needed.

Methods

The cell viability of T98G cells was detected by cell counting kit-8 (CCK-8) assay. Apoptosis was detected using the Annexin-V-FITC & PI apoptosis kit and assessed by flow cytometry. The expression levels of Bax, B cell lymphoma 2 (Bcl-2), phos-Jun N-terminal kinases (JNK), phos-extracellular signal–regulated kinases (ERK) and phos-p38 were determined by western blot. The effect of ultrasound and temozolomide combination in mice was determined by survival analysis.

Results

Compared with temozolomide treatment alone, ultrasound and temozolomide combination inhibited the cell viability, and promotes apoptosis of human glioblastoma T98G cells. Bax level increased, while Bcl-2 level decreased in combination group. Mechanically, combination treatment promoted apoptosis via JNK and p38 pathways. In mouse glioblastoma model, combination treatment improved overall survival.

Conclusions

Ultrasound enhanced anti-tumor effect of temozolomide in glioblastoma cells via JNK and p38 pathways.

Keywords

Glioblastoma Ultrasound Temozolomide Apoptosis JNK and p38 pathways 

Notes

Conflict of interest

Jie Xue, Yuanyuan Wu, and Na Liu declare that declare that they have no conflicts of interest.

Ethical Standards

No human studies were carried out by the authors for this article. All animal studies were carried out in accordance with the institutional guidelines and approved by the ethics committee of Yantai Yuhuangding Hospital.

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Copyright information

© Biomedical Engineering Society 2018

Authors and Affiliations

  1. 1.Department of UltrasonographyYantai Yuhuangding HospitalYantaiChina
  2. 2.Department of UltrasoundCentral Hospital of WeihaiWeihaiChina
  3. 3.Department of Special InspectionYantai Yeda Hospital, Yantai Economic and Technological Development AreaYantaiChina

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