Cellular and Molecular Bioengineering

, Volume 12, Issue 1, pp 85–97 | Cite as

Platelet-Derived Growth Factor Receptor-α and β are Involved in Fluid Shear Stress Regulated Cell Migration in Human Periodontal Ligament Cells

  • Lisha ZhengEmail author
  • Qiusheng Shi
  • Jing Na
  • Nan Liu
  • Yuwei Guo
  • Yubo FanEmail author



Fluid shear stress (FSS) is the most common stress produced by mastication, speech, or tooth movement. However, how FSS regulates human periodontal ligament (PDL) cell proliferation and migration as well as the underlying mechanism remains unknown.


FSS (6 dyn/cm2) was produced in a flow chamber. Cell proliferation was tested by the 5-ethynyl-2′-deoxyuridine assay. Cell migration was tested by the wound healing assay. Gene and protein expression of platelet-derived growth factors (PDGFs) and matrix metalloproteinase (MMP)-2 were measured by reverse transcription-polymerase chain reaction and western blot analyses.


We investigated the effect of 4 h of 6 dyn/cm2 FSS on proliferation and migration of PDL cells. FSS promoted PDL cell proliferation but inhibited migration. The gene and protein expression of PDGF receptor (PDGFR)-α and β both decreased in response to FSS. Activating and inhibiting the PDGFRs did not affect the FSS-induced increase in cell proliferation. However, activating PDGFRs with PDGF-BB, which bound both PDGFR-α and β, and PDGF-CC and DD, which had high affinities for PDGFR-α and PDGFR-β, individually rescued FSS-inhibited migration. FSS also inhibited MMP-2 gene expression, which was the most important factor for matrix turnover and migration of PDLs. PDGF-BB, CC, and DD increased the FSS-induced decline in MMP-2 expression. These results indicate that MMP-2 is regulated by FSS and contributes to the FSS-induced decrease in cell migration.


Our study suggests a role for PDGFR-α and β in short-term FSS-regulated cell proliferation and migration. These results will help provide the scientific foundation for revealing the mechanisms clinical tooth movement and PDL regeneration.


PDGF receptor Proliferation Migration Matrix metalloproteinases-2 



Periodontal ligament


Extracellular matrix


Platelet-derived growth factors


Fluid shear stress


Mitogen-activated protein kinase


Phosphoinositide 3-kinase


Phospholipase C


Matrix metalloproteinases


Dulbecco’s modified Eagle medium


Fetal bovine serum




Phenylmethane sulfonyl fluoride


Sodium dodecyl sulfate polyacrylamide gel electrophoresis


Polyvinylidene fluoride


Triethanolamine buffered saline solution


Horseradish peroxidase


Enhanced chemiluminescent


Mesenchymal stem cell


Smooth muscle cell



This work was supported by National Natural Science Foundation of China (Grant Numbers 11572030), National Key R&D Program of China (2017YFC0108505); National Natural Science Foundation of China (Grant Numbers 11120101001, 11421202); the Fundamental Research Funds for the Central Universities; the 111 Project (Grant Numbers B13003).

Conflict of interest

All authors including Lisha Zheng, Qiusheng Shi, Jing Na, Nan Liu, Yuwei Guo, Yubo Fan have no conflicts of interest.

Ethical Approval

The authors state that all human subjects research was carried out in accordance with the Declaration of Helsinki and approved by Beihang University Ethics Committee. This study did not involve any animal research.


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Copyright information

© Biomedical Engineering Society 2018

Authors and Affiliations

  1. 1.School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of EducationBeijingChina
  2. 2.Beijing Advanced Innovation Centre for Biomedical EngineeringBeihang UniversityBeijingChina
  3. 3.National Research Center for Rehabilitation Technical AidsBeijingChina
  4. 4.School of Biological Science and Medical EngineeringBeijingChina

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