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Cellular and Molecular Bioengineering

, Volume 12, Issue 1, pp 85–97 | Cite as

Platelet-Derived Growth Factor Receptor-α and β are Involved in Fluid Shear Stress Regulated Cell Migration in Human Periodontal Ligament Cells

  • Lisha ZhengEmail author
  • Qiusheng Shi
  • Jing Na
  • Nan Liu
  • Yuwei Guo
  • Yubo FanEmail author
Article

Abstract

Introduction

Fluid shear stress (FSS) is the most common stress produced by mastication, speech, or tooth movement. However, how FSS regulates human periodontal ligament (PDL) cell proliferation and migration as well as the underlying mechanism remains unknown.

Methods

FSS (6 dyn/cm2) was produced in a flow chamber. Cell proliferation was tested by the 5-ethynyl-2′-deoxyuridine assay. Cell migration was tested by the wound healing assay. Gene and protein expression of platelet-derived growth factors (PDGFs) and matrix metalloproteinase (MMP)-2 were measured by reverse transcription-polymerase chain reaction and western blot analyses.

Results

We investigated the effect of 4 h of 6 dyn/cm2 FSS on proliferation and migration of PDL cells. FSS promoted PDL cell proliferation but inhibited migration. The gene and protein expression of PDGF receptor (PDGFR)-α and β both decreased in response to FSS. Activating and inhibiting the PDGFRs did not affect the FSS-induced increase in cell proliferation. However, activating PDGFRs with PDGF-BB, which bound both PDGFR-α and β, and PDGF-CC and DD, which had high affinities for PDGFR-α and PDGFR-β, individually rescued FSS-inhibited migration. FSS also inhibited MMP-2 gene expression, which was the most important factor for matrix turnover and migration of PDLs. PDGF-BB, CC, and DD increased the FSS-induced decline in MMP-2 expression. These results indicate that MMP-2 is regulated by FSS and contributes to the FSS-induced decrease in cell migration.

Conclusions

Our study suggests a role for PDGFR-α and β in short-term FSS-regulated cell proliferation and migration. These results will help provide the scientific foundation for revealing the mechanisms clinical tooth movement and PDL regeneration.

Keywords

PDGF receptor Proliferation Migration Matrix metalloproteinases-2 

Abbreviations

PDL

Periodontal ligament

ECM

Extracellular matrix

PDGF

Platelet-derived growth factors

FSS

Fluid shear stress

MAPK

Mitogen-activated protein kinase

PI3K

Phosphoinositide 3-kinase

PLC

Phospholipase C

MMP

Matrix metalloproteinases

DMEM

Dulbecco’s modified Eagle medium

FBS

Fetal bovine serum

EdU

5-Ethynyl-2′-deoxyuridine

PMSF

Phenylmethane sulfonyl fluoride

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

PVDF

Polyvinylidene fluoride

TBS-T

Triethanolamine buffered saline solution

HRP

Horseradish peroxidase

ECL

Enhanced chemiluminescent

MSC

Mesenchymal stem cell

SMC

Smooth muscle cell

Notes

Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant Numbers 11572030), National Key R&D Program of China (2017YFC0108505); National Natural Science Foundation of China (Grant Numbers 11120101001, 11421202); the Fundamental Research Funds for the Central Universities; the 111 Project (Grant Numbers B13003).

Conflict of interest

All authors including Lisha Zheng, Qiusheng Shi, Jing Na, Nan Liu, Yuwei Guo, Yubo Fan have no conflicts of interest.

Ethical Approval

The authors state that all human subjects research was carried out in accordance with the Declaration of Helsinki and approved by Beihang University Ethics Committee. This study did not involve any animal research.

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Copyright information

© Biomedical Engineering Society 2018

Authors and Affiliations

  1. 1.School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of EducationBeijingChina
  2. 2.Beijing Advanced Innovation Centre for Biomedical EngineeringBeihang UniversityBeijingChina
  3. 3.National Research Center for Rehabilitation Technical AidsBeijingChina
  4. 4.School of Biological Science and Medical EngineeringBeijingChina

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