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Effects of abiotic stress on the expression of Hsp70 genes in Sogatella furcifera (Horváth)

  • Cao Zhou
  • Xi-bin Yang
  • Hong YangEmail author
  • Gui-yun Long
  • Zhao Wang
  • Dao-chao Jin
Original Paper
  • 27 Downloads

Abstract

Sogatella furcifera (Horváth), a prominent rice pest in Asia, is a typical R-strategic and highly adaptable insect. Heat shock proteins (Hsps) are highly conserved molecular chaperones regulating responses to various abiotic stresses; however, limited information is available regarding their role in responding to abiotic stress in S. furcifera. This study aimed to investigate the effect of abiotic stresses on the expression of Hsp70 genes in the S. furcifera. Five Hsp70 genes were isolated from S. furcifera, and the expression patterns at different developmental stages and temperatures, upon treatment with different insecticides and ultraviolet A (UV-A) stress, were analyzed. Hsp70 genes were expressed at different developmental stages. Hsp70-2, Hsp70-5, and Hsp70-6 were significantly upregulated upon heat shock at 40 °C for 30 min. Hsp70-3 and Hsp70-4 were significantly upregulated upon heat shock at 30 °C for 30 min. Under UV-A stress, Hsp70-3, Hsp70-4, Hsp70-5, and Hsp70-6 were significantly upregulated. Conversely, Hsp70-2 was significantly downregulated under UV-A stress. The five Hsp70 genes were significantly downregulated in 3rd-instar nymphs on exposure to thiamethoxam, buprofezin, and avermectin at LC10 and LC25 concentrations. Hence, Hsp70 genes significantly contribute to the tolerance of S. furcifera to temperature and UV-A stress; however, they are not involved in the response to insecticides.

Keywords

Sogatella furcifera Temperature Ultraviolet A radiation Insecticides Heat shock protein 

Notes

Funding information

This study was supported by the National Natural Science Foundation of China (Grant No. 31560522 and 31960537), Provincial Key Project for Agricultural Science and Technology of Guizhou (NY20133006 and NY20103064), International Cooperation Base for Insect Evolutionary Biology and Pest Control ([2016]5802), and Graduate Education Innovation Project of Guizhou Province (Qian Jiao He YJSCXJH, No. [2018]043).

Supplementary material

12192_2019_1053_MOESM1_ESM.docx (29 kb)
ESM 1 (DOCX 28 kb)

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Copyright information

© Cell Stress Society International 2019

Authors and Affiliations

  1. 1.Institute of Entomology, Provincial Key Laboratory for Agricultural Pest Management of Mountainous RegionsGuizhou UniversityGuiyangPeople’s Republic of China
  2. 2.College of Tobacco ScienceGuizhou UniversityGuiyangPeople’s Republic of China
  3. 3.College of Environment and Life SciencesKaili UniversityKailiPeople’s Republic of China

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