Cell Stress and Chaperones

, Volume 24, Issue 3, pp 549–565 | Cite as

Molecular basis for efficacy of Guduchi and Madhuyashti feeding on different environmental stressors in Drosophila

  • Surabhi Singh
  • Madhu G. TapadiaEmail author
Original Paper


Stressors of different kinds adversely affect life history parameters like growth, development, and reproduction. Organisms overcome the negative impact of environmental stressors and strive to reach a tolerant state through genetic and metabolic activities. Ayurvedic formulations are reported to have life trait benefitting properties which improve capacity to withstand stress and tolerate adverse conditions. Guduchi (Tinospora cordifolia) and Madhuyashti (Glycirrhiza glabra) Ayurvedic formulations are known to have immunomodulatory, intellect promoting, and adaptogenic properties, thus favoring good health and healthy aging. Present study investigates the efficacy of Guduchi and Madhuyashti in providing tolerance to different stresses and the underlying mechanisms using the Drosophila model. Drosophila larvae/flies fed on Guduchi or Madhuyashti were better thermo-protected, which correlated with increased expression of heat shock genes even without the heat shock. Guduchi or Madhuyashti feeding also increased antimicrobial peptide expression, thus providing better tolerance to pathogenic assaults. Feeding on Guduchi- or Madhuyashti- supplemented food also enhanced starvation and desiccation tolerance. However, neither of these formulations provided beneficial effects when grown under crowded conditions or when exposed to oxidative stressors.


Ayurveda Guduchi Madhuyashti Heat shock proteins ROS Thermotolerance Starvation Desiccation 



We acknowledge Arya Vaidya Sala, (Kotakkal, Kerala, India) for providing the Ayurvedic formulations. We also thank Department of Science and Technology and the Banaras Hindu University for the confocal microscopy facility. Our sincere thanks to Dr. M. B. Evgen’ev (Russia) for the anti-Hsp70 7Fb antibody. We are extremely grateful to Prof. S. C. Lakhotia for his intellectual inputs and carefully editing the manuscript. We would also like to thank the editor and anonymous reviewers for their valuable comments which greatly helped in preparation of the manuscript.

Funding information

This work is supported by grants from the Department of Science and Technology, Science and Engineering Research Board (SERB), Government of India, New Delhi, to Madhu.G.Tapadia and JRF to Surabhi Singh. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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© Cell Stress Society International 2019

Authors and Affiliations

  1. 1.Cytogenetics Laboratory, Department of ZoologyBanaras Hindu UniversityVaranasiIndia

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