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Cell Stress and Chaperones

, Volume 24, Issue 1, pp 91–104 | Cite as

Lipopolysaccharide induces human olfactory ensheathing glial apoptosis by promoting mitochondrial dysfunction and activating the JNK-Bnip3-Bax pathway

  • Maowei He
  • Zimin Xiang
  • Libin Xu
  • Yanting Duan
  • Fangqin Li
  • Jianmei ChenEmail author
Original Paper

Abstract

Olfactory ensheathing glia (OEG) play an important role in regulating the regeneration of an injured nervous system. However, chronic inflammation damage reduces the viability of OEG via poorly understood mechanisms. We aimed to investigate the pathological responses of OEG in response to LPS-mediated inflammation stress in vitro. The results indicated that lipopolysaccharide (LPS) treatment significantly reduced the viability of OEG in a dose-dependent fashion. Mechanistically, LPS stimuli induced mitochondrial oxidative damage, mitochondrial fragmentation, mitochondrial metabolism disruption, and mitochondrial apoptosis activation. Furthermore, we verified that LPS modulated mitochondrial apoptosis by promoting Bax upregulation, and this process was regulated by the JNK-Bnip3 pathway. Inhibition of the JNK-Bnip3 pathway prevented LPS-mediated Bax activation, thus attenuating OEG apoptosis. Altogether, our data illustrated that LPS-mediated inflammation injury evoked mitochondrial abnormalities in OEG damage via the JNK-Bnip3-Bax pathway. This finding provides a potential target to protect OEG against chronic inflammation stress.

Keywords

Olfactory ensheathing glia Mitochondrial dysfunction Bax JNK-Bnip3 pathway 

Notes

Authors’ contributions

MWH, ZMX, and JMC were involved in the conception and design, performance of experiments, data analysis and interpretation, and manuscript writing. LBX, YTD, FQL, and JMC were involved in data analysis and interpretation.

Funding

This study was supported by grants from the major project of logistic scientific Research of PLA (AWS14C003–5).

Compliance with ethical standards

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Supplementary material

12192_2018_945_MOESM1_ESM.docx (427 kb)
ESM 1 (DOCX 426 kb)

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Copyright information

© Cell Stress Society International 2018

Authors and Affiliations

  • Maowei He
    • 1
  • Zimin Xiang
    • 2
  • Libin Xu
    • 2
  • Yanting Duan
    • 1
  • Fangqin Li
    • 3
  • Jianmei Chen
    • 2
    Email author
  1. 1.Bengbu Medical CollegeAffiliated Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLAFuzhouChina
  2. 2.Department of OrthopedicsFuzhou General Hospital of Nanjing Military Area Command of Chinese PLAFuzhouChina
  3. 3.Fujian University of Traditional Chinese MedicineFuzhouChina

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