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Cell Stress and Chaperones

, Volume 24, Issue 1, pp 45–58 | Cite as

RNA-Seq analysis of Polyrhachis vicina Roger and insights into the heat shock protein 90 and 70 families

  • JuanJuan Zhang
  • GengSi XiEmail author
  • ZhiYi Guo
  • FengHua Jia
Original Paper
  • 67 Downloads

Abstract

The heat shock protein 90 (Hsp90) and heat shock cognate proteins (Hsc70) have been identified as chaperones of the ecdysone receptor (EcR)/ultraspiracle protein (USP) heterocomplex. However, little is known about the status of Hsp90 and Hsc70 in Polyrhachis vicina Roger. Here, we sequenced the transcriptomes of adult ants in P. vicina for the first time. Clean reads in female, male, and worker ants were annotated into 40,147 transcripts, and 37,488, 28,300, and 33,638 unigenes were assembled in female, male, and worker ants, respectively. According to RPKM, the numbers of differentially expressed genes between female and male ants, between female and worker ants, and between male and worker ants and the common differentially expressed genes were 12,657, 21,630, 15,112 and 3704, respectively. These results reveal that caste differentiation, caste specificity formation, and social divisions of P. vicina ants may be due to gene expression differences. Moreover, PvEcR and PvUSP were also detected as differentially expressed genes in the ants; specifically, PvUSP expression was higher than PvEcR expression in all castes. We speculate that PvUSP may have a role similar to that of juvenile hormone receptor. Four identified PvHsp90 family members and 23 identified PvHsp70 family members were found in the ants, and 2 PvHsp90 genes and 8 PvHsp70 genes were analyzed by qRT-PCR. Among those genes, the expression of 2 PvHsp90 genes and 5 PvHsp70 genes coincided with the expression profiles of PvEcR and PvUSP, which suggest that the characterization of PvHsp90 and PvHsc70 may be as EcR/USP molecular chaperones in P. vicina.

Keywords

Polyrhachis vicina Roger Transcriptome Heat shock protein 90 family Heat shock protein 70 family Differentially expressed genes 

Notes

Acknowledgements

We thank Wang X and Liu C for comments on this manuscript.

Funding information

This work was supported by grants from the National Natural Science Foundation of China (Nos. 31571276 and 31171195).

Supplementary material

12192_2018_940_Fig8_ESM.png (49 kb)
Fig. S1

Sequence length distribution. The sequence length distribution are shown as a percentage of the total unigenes. The corresponding proportion are marked on each column. (PNG 48.9 kb)

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High resolution image (TIF 75.4 kb)
12192_2018_940_Fig9_ESM.png (947 kb)
Fig. S2

Homology search of unigenes against the non-redundant protein sequence (nr) database. a: E-value distribution, b: Species distributions. (PNG 946 kb)

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High resolution image (TIF 1.48 mb)
12192_2018_940_Fig10_ESM.png (366 kb)
Fig. S3

Histogram of GO classifications of unigenes. The results are summarized in three main categories: biological process, cellular components, and molecular functions. (PNG 366 kb)

12192_2018_940_MOESM3_ESM.tif (1.6 mb)
High resolution image (TIF 1.60 mb)
12192_2018_940_MOESM4_ESM.pdf (59 kb)
ESM 4 (PDF 59.1 kb)
12192_2018_940_MOESM5_ESM.pdf (63 kb)
ESM 5 (PDF 62.7 kb)
12192_2018_940_MOESM6_ESM.pdf (63 kb)
ESM 6 (PDF 62.8 kb)

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Copyright information

© Cell Stress Society International 2018

Authors and Affiliations

  • JuanJuan Zhang
    • 1
  • GengSi Xi
    • 1
    Email author
  • ZhiYi Guo
    • 1
  • FengHua Jia
    • 1
  1. 1.Institute of Zoology, College of Life ScienceShaanxi Normal UniversityXi’anPeople’s Republic of China

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