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Cell Stress and Chaperones

, Volume 23, Issue 4, pp 703–710 | Cite as

Pre-protective effects of dietary chitosan supplementation against oxidative stress induced by diquat in weaned piglets

  • Y. Q. Xu
  • Y. Y. Xing
  • Z. Q. Wang
  • S. M. Yan
  • B. L. Shi
Original Paper

Abstract

The protective effects of chitosan (CS) supplementations on oxidative stress induced by diquat in weaned piglets were investigated. A total of 36 crossbreed piglets with an average live body weight (BW) of 8.80 ± 0.53 kg were weaned at 28 ± 2 days and randomly divided into six dietary treatments (n = 6): control (basal diet), negative control (10 mg diquat/kg BW injected to piglets fed with basal diet), and basal diet treatments containing either 250, 500, 1000, or 2000 mg/kg of CS administered to piglets injected with 10 mg diquat/kg BW. The experiment conducted for 21 days which consisted of pre-starter period (14 days) and starter period (7 days). BW, feed intake, and fecal consistency were monitored. Blood samples were collected to determine antioxidative and immune parameters. CS supplementation improved the growth performance and decreased fecal score of piglets from days 1 to 14. Diquat also induced oxidative stress and inflammatory responses by decreasing the activities of antioxidant and regulating cytokines. But dietary CS alleviated these negative effects induced by diquat that showed decreasing serum concentrations of pro-inflammatory cytokines but increasing activities of antioxidant enzymes and anti-inflammatory cytokines. Results indicated that CS attenuated the oxidative stress of piglets caused by diquat injection.

Keywords

Chitosan Growth performance Diarrhea Antioxidant capacity Cytokine 

Notes

Acknowledgements

For their help during laboratory and data analysis, the authors express deep appreciation to Y.X. Yue and Z. Qin from College of Animal Science, Inner Mongolia Agricultural University, China.

Funding information

This work was supported by grants of National Natural Science Foundation of China (Project No. 31460605).

Compliance with ethical standards

The experimentation was carried out at the experimental farms of the Inner Mongolia Agricultural University in China and was approved by the Animal Care and Use Committee of Inner Mongolia Agricultural University.

References

  1. Anandan R, Ganesan B, Obulesu T et al (2012) Dietary chitosan supplementation attenuates isoprenaline-induced oxidative stress in rat myocardium. Int J Biol Macromol 51:783–787CrossRefPubMedGoogle Scholar
  2. Baek KS, Won EK, Choung SY (2007) Effects of chitosan on serum cytokine levels in elderly subjects. Arch Pharm Res 12:1550–1557CrossRefGoogle Scholar
  3. Bonnette ED, Kornegay ET, Lindemann MD et al (1990) Influence of two supplemental vitamin E levels and weaning age on performance, humoral antibody production and serum cortisol levels of pigs. J Anim Sci 68:1346–1353CrossRefPubMedGoogle Scholar
  4. Burton AB, Wagner B, Erb HN et al (2009) Serum interleukin-6 (il-6) and il-10 concentrations in normal and septic neonatal foals. Vet Immunol Immunopathol 132:122–128CrossRefPubMedGoogle Scholar
  5. David AZ, Connie JR, Kenneth WH et al (2007) Chitosan solution enhances both humoral and cell-mediated immune responses to subcutaneous vaccination. Vaccine 11:2085–2094Google Scholar
  6. Deng Q, Xu J, Yu B et al (2010) Effect of dietary tea polyphenols on growth performance and cell-mediated immune response of post-weaning piglets under oxidative stress. Arch Anim Nutr 64:12–21CrossRefPubMedGoogle Scholar
  7. Durackova Z (2010) Some current insights into oxidative stress. Physiol Res 59:459–469PubMedGoogle Scholar
  8. Eicher SD, McKee CA, Carroll JA et al (2006) Supplemental vitamin C and yeast cell wall β-glucan as growth enhancers in newborn pigs and as immunomodulators after an endotoxin challenge after weaning. J Anim Sci 84:2352–2360CrossRefPubMedGoogle Scholar
  9. Epinat JC, Gilmore TD (1999) Diverse agents act at multiple levels to inhibit the Rel/NF-κB signal transduction pathway. Oncogene 18:6896–6909CrossRefPubMedGoogle Scholar
  10. Feng T, Du YM, Li J et al (2008) Enhancement of antioxidant activity of chitosan by irradiation. Carbohyd Polym 73:126–132CrossRefGoogle Scholar
  11. Gabay C, Kushner I (1999) Acute-phase proteins and other systemic responses to inflammation. N Engl J Med 340:448–454CrossRefPubMedGoogle Scholar
  12. Han J, Shuvaev VV, Muzykantov VR (2011) Catalase and SOD conjugated with PECAM antibody distinctly alleviate abnormal endothelial permeability caused by exogenous ROS and vascular endothelial growth factor. J Pharmacol Exp Ther 338:82–91CrossRefPubMedPubMedCentralGoogle Scholar
  13. Han YH, Moon HJ, Br Y et al (2009) The effect of MAPK inhibitors on arsenic trioxide-treated Calu-6 lung cells in relation to cell death. ROS and GSH levels Anticancer Res 29:3837–3844PubMedGoogle Scholar
  14. Huang TT, Miyamoto S (2001) Postrepression activation of NF-κB requires the amino-terminal nuclear export signal specific to IκBα. Mol Cell Biol 21:4737–4747CrossRefPubMedPubMedCentralGoogle Scholar
  15. Hussain SP, Hofseth LJ, Harris CC (2003) Radical causes of cancer. Nat Rev Cancer 3:276–285CrossRefPubMedGoogle Scholar
  16. Kadenbach B, Ramzan R, Vogt S (2009) Degenerative diseases, oxidative stress and cytochrome c oxidase function. Trends Mol Med 15:139–147CrossRefPubMedGoogle Scholar
  17. Kudielka BM, Kirschbaum CK, Kirschbaum C (2005) Sex differences in HPA axis responses to stress: a review. Biol Psychol 69:113–132CrossRefPubMedGoogle Scholar
  18. Kumar S, Pandey AK (2015) Free radicals: health implications and their mitigation by herbals. Br J Med Med Res 7:438–457CrossRefGoogle Scholar
  19. Li JL, Shi BL, Yan SM et al (2013) Effects of dietary supplementation of chitosan on stress hormones and antioxidative enzymes in weaned piglets. J Anim Vet Adv 12:650–654Google Scholar
  20. Li T, Na R, Yu P et al (2015) Effects of dietary supplementation of chitosan on immune and antioxidative function in beef cattle. Czech J Anim Sci 60:38–44CrossRefGoogle Scholar
  21. Lu T, Piao XL, Zhang Q et al (2010) Protective effects of Forsythia suspensa extract against oxidative stress induced by diquat in rats. Food Chem Toxicol 48:764–770CrossRefPubMedGoogle Scholar
  22. Lv M, Yu B, Mao XB et al (2012) Responses of growth performance and tryptophan metabolism to oxidative stress induced by diquat in weaned pigs. Animal 6:928–934CrossRefPubMedGoogle Scholar
  23. Malek S, Chen Y, Huxford T et al (2001) IκBβ, but not IκBα, functions as a classical cytoplasmic inhibitor of NF-κB dimers by masking both NF-κB nuclear localization sequences in resting cells. J Biol Chem 276:45225–45235CrossRefPubMedGoogle Scholar
  24. Mendis E, Kim MM, Rajapakse N et al (2007) An in vitro cellular analysis of the radical scavenging efficacy of chitooligosaccharides. Life Sci 80:2118–2127CrossRefPubMedGoogle Scholar
  25. Mormède P, Andanson S, Aupérin B et al (2007) Exploration of the hypothalamic-pituitary-adrenal function as a tool to evaluate animal welfare. Physiol Behav 92:317–339CrossRefPubMedGoogle Scholar
  26. NRC (2012) Nutrient Requirements of Swine. 11th ed. Washington DC, National academy pressGoogle Scholar
  27. Pierce KM, Callan JJ, McCarthy P, O’Doherty JV (2005) Performance of weanling pigs offered low or high lactose diets supplemented with avilamycin or inulin. Anim Sci 80:313–318CrossRefGoogle Scholar
  28. Pirinccioglu AG, Gökalp D, Pirinccioglu M et al (2010) Malondialdehyde (MDA) and protein carbonyl (PCO) levels as biomarkers of oxidative stress in subjects with familial hypercholesterolemia. Clin Biochem 43:1220–1224CrossRefPubMedGoogle Scholar
  29. Poyton RO, Ball KA, Castello PR (2009) Mitochondrial generation of free radicals and hypoxic signaling. Trends Endocrin Me 20:332–340CrossRefGoogle Scholar
  30. Pusterla N, Magdesian KG, Mapes S et al (2006) Expression of molecular markers in blood of neonatal foals with sepsis. Am J Vet Res 67:1045–1049CrossRefPubMedGoogle Scholar
  31. Qiang M, Xu YJ, Lu Y et al (2014) Autofluorescence of MDA-modified proteins as an in vitro and in vivo probe in oxidative stress analysis. Protein Cell 5:484–487CrossRefPubMedPubMedCentralGoogle Scholar
  32. Qiao Y, Bai XF, Du YG (2011) Chitosan oligosaccharides protect mice from LPS challenge by attenuation of inflammation and oxidative stress. Int Immunopharmacol 11:121–127CrossRefPubMedGoogle Scholar
  33. Rajat G, Panchali D (2014) A study on antioxidant properties of different bioactive compounds. J Drug Deliv Ther 4:105–115Google Scholar
  34. Seeley EJ, Matthay MA, Wolters PJ (2012) Inflection points in sepsis biology: from local defense to systemic organ injury. Am J Physiol Lung Cell Mol Physiol 303:L355–L363CrossRefPubMedPubMedCentralGoogle Scholar
  35. Tao Y, Zhang HL, Hu YM et al (2013) Preparation of chitosan and water-soluble shitosan microspheres via spray-drying method to lower blood lipids in rats fed with high-fat diets. Int J Mol Sci 14:4174–4184CrossRefPubMedPubMedCentralGoogle Scholar
  36. Tokura S, Tamura H, Azuma I (1999) Immunological aspects of chitin and chitin derivatives administered to animals. EXS 87:279–292PubMedGoogle Scholar
  37. Valko M, Leibfritz D, Moncol J et al (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84CrossRefPubMedGoogle Scholar
  38. Villiers C, Chevallet M, Diemer H et al (2009) From secretome analysis to immunology chitosan induces major alterations in the activation of dendritic cells via a TLR-4 dependent mechanism. Mol Cell Proteomics 8:1252–1264CrossRefPubMedPubMedCentralGoogle Scholar
  39. Wang C, Schuller Levis GB, Lee EB et al (2004) Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-α in activated RAW 264.7 cells. Int Immunopharmacol 4:1039–1049CrossRefPubMedGoogle Scholar
  40. West CE, Gothefors L, Granstrom M et al (2008) Effects of feeding probiotics during weaning on infections and antibody responses to diphtheria, tetanus and Hib vaccines. Pediatr Allergy Immunol 19:53–60CrossRefPubMedGoogle Scholar
  41. Williams BA, Verstegen MWA, Tamminga S (2001) Fermentation in the large intestine of single-stomached animals and its relationship to animal health. Nutr Res Rev 14:207–227CrossRefPubMedGoogle Scholar
  42. Xiong QY, Wei YN, Xie HD et al (2014) Effect of different adjuvant formulations on the immunogenicity and protective effect of a live Mycoplasma hyopneumoniae vaccine after intramuscular inoculation. Vaccine 32:3445–3451CrossRefPubMedGoogle Scholar
  43. Xiong X, Yang HS, Wang XC et al (2015) Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets. J Anim Sci 93:1089–1097CrossRefPubMedGoogle Scholar
  44. Xu YQ, Wang ZQ, Shi BL et al (2017) Effects of chitosan on growth performance, fecal score, serum hormones and T lymphocyte subset of weaned piglets. Chin J Anim Nutr 29:1678–1686Google Scholar
  45. Yin YL, Tang ZR, Sun ZH et al (2008) Effect of galacto-mannan-oligosaccharides or chitosan supplementation on cytoimmunity and humoral immunity in early-weaned piglets. Asian-Australas J Anim Sci 21:723–731CrossRefGoogle Scholar
  46. Yuan SB, Chen DW, Zhang KY et al (2007) Effects of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidative enzymes of weanling pigs. Asian-Australas J Anim Sci. 20:1600–1605CrossRefGoogle Scholar
  47. Zhu LH, Zhao KL, Chen XL et al (2012) Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs. J Anim Sci 90:2581–2589CrossRefPubMedGoogle Scholar

Copyright information

© Cell Stress Society International 2018

Authors and Affiliations

  • Y. Q. Xu
    • 1
  • Y. Y. Xing
    • 1
  • Z. Q. Wang
    • 1
  • S. M. Yan
    • 1
  • B. L. Shi
    • 1
  1. 1.College of Animal ScienceInner Mongolia Agricultural UniversityHohhotChina

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