Cell Stress and Chaperones

, Volume 23, Issue 4, pp 703–710 | Cite as

Pre-protective effects of dietary chitosan supplementation against oxidative stress induced by diquat in weaned piglets

  • Y. Q. Xu
  • Y. Y. Xing
  • Z. Q. Wang
  • S. M. Yan
  • B. L. ShiEmail author
Original Paper


The protective effects of chitosan (CS) supplementations on oxidative stress induced by diquat in weaned piglets were investigated. A total of 36 crossbreed piglets with an average live body weight (BW) of 8.80 ± 0.53 kg were weaned at 28 ± 2 days and randomly divided into six dietary treatments (n = 6): control (basal diet), negative control (10 mg diquat/kg BW injected to piglets fed with basal diet), and basal diet treatments containing either 250, 500, 1000, or 2000 mg/kg of CS administered to piglets injected with 10 mg diquat/kg BW. The experiment conducted for 21 days which consisted of pre-starter period (14 days) and starter period (7 days). BW, feed intake, and fecal consistency were monitored. Blood samples were collected to determine antioxidative and immune parameters. CS supplementation improved the growth performance and decreased fecal score of piglets from days 1 to 14. Diquat also induced oxidative stress and inflammatory responses by decreasing the activities of antioxidant and regulating cytokines. But dietary CS alleviated these negative effects induced by diquat that showed decreasing serum concentrations of pro-inflammatory cytokines but increasing activities of antioxidant enzymes and anti-inflammatory cytokines. Results indicated that CS attenuated the oxidative stress of piglets caused by diquat injection.


Chitosan Growth performance Diarrhea Antioxidant capacity Cytokine 



For their help during laboratory and data analysis, the authors express deep appreciation to Y.X. Yue and Z. Qin from College of Animal Science, Inner Mongolia Agricultural University, China.

Funding information

This work was supported by grants of National Natural Science Foundation of China (Project No. 31460605).

Compliance with ethical standards

The experimentation was carried out at the experimental farms of the Inner Mongolia Agricultural University in China and was approved by the Animal Care and Use Committee of Inner Mongolia Agricultural University.


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Copyright information

© Cell Stress Society International 2018

Authors and Affiliations

  • Y. Q. Xu
    • 1
  • Y. Y. Xing
    • 1
  • Z. Q. Wang
    • 1
  • S. M. Yan
    • 1
  • B. L. Shi
    • 1
    Email author
  1. 1.College of Animal ScienceInner Mongolia Agricultural UniversityHohhotChina

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