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Cell Stress and Chaperones

, Volume 23, Issue 4, pp 791–796 | Cite as

HSP60 expression profile under different extreme temperature stress in albino northern snakehead, Channa argus

  • Aiguo Zhou
  • Shaolin Xie
  • Zhenlu Wang
  • Yanfeng Chen
  • Yue Zhang
  • Lanfen Fan
  • Fang Zeng
  • Jixing Zou
Short Communication
  • 107 Downloads

Abstract

The great albino northern snakehead, Channa argus, is one of the most important economical fish in China. In the present study, cDNA encoding heat shock protein 60 (HSP60) was cloned and characterized. The cDNA was 2462 bp, containing an open reading frame (ORF) encoding a 575-amino-acids polypeptide of 61.10 kDa (theoretical isoelectric point [pI]: 5.66). BLAST analysis showed that AcaHSP60 was highly similar with other HSP60s, and three conserved amino acid blocks and characteristic motifs or domains defined as HSP60 protein family signatures. Genomic DNA analysis showed that AcaHSP60 had ten exons in the coding region (from 94 to 336 bp). Changes in AcaHSP60 gene expression profiles in albino C. argus experimentally exposed to different temperature stress (8.5, 26, and 37 °C) was investigated. Quantitative real-time PCR and western blot analysis revealed that tissue-specific AcaHSP60 expressions were in the spleen, muscle, liver, kidney, heart and brain. Expression was highly significantly stimulated after heat shock (37 °C), but showed no significant differences after cold treatment (8.5 °C) except in the brain. In summary, these results showed that AcaHSP60 was significantly tissue specific and indicate that AcaHSP60 expression might be sensitive to thermal resistance in albino C. argus.

Keywords

Albino Channa argus Heat shock protein 60 Temperature stress Gene expression 

Notes

Acknowledgements

We express our appreciation to our anonymous reviewers for providing valuable comments on the manuscript.

Funding information

This work was supported by the Science and Technology Planning Project of Guangdong Province (2017A020225035; 2016A020210141); Produce-learn-research Project of Guangdong Province (2011B090400270); Fund Fostering Talents for Young Scholars of South China Agricultural University (201707 N025); Talent introduction special funds of South China Agricultural University and Scientific Research Staring Foundation for Young Scholars of College of Marine Sciences.

Compliance with ethical standards

All of the procedures and animal handling were performed in accordance with the guide for the Chinese Association for Laboratory Animal Sciences. Approval of the study was obtained from the Animal Ethics Committee of South China Agricultural University.

The authors alone are responsible for the content and writing of this article.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

12192_2017_869_MOESM1_ESM.docx (14 kb)
ESM 1 (DOCX 13 kb)

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Copyright information

© Cell Stress Society International 2017

Authors and Affiliations

  • Aiguo Zhou
    • 1
    • 2
  • Shaolin Xie
    • 1
    • 2
  • Zhenlu Wang
    • 1
  • Yanfeng Chen
    • 3
  • Yue Zhang
    • 4
  • Lanfen Fan
    • 1
  • Fang Zeng
    • 1
  • Jixing Zou
    • 1
  1. 1.College of Marine SciencesSouth China Agricultural UniversityGuangzhouChina
  2. 2.Qingyuan North River Fishery Science InstituteQingyuanChina
  3. 3.School of Life Science and EngineeringFoshan UniversityFoshanChina
  4. 4.Departments of Pharmacology and Pharmaceutical Sciences, School of PharmacyUniversity of Southern CaliforniaLos AngelesUSA

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