Colloidal gold, quantum dots (QDs), and upconversion nanoparticles (UCNPs), which were respectively labeled with monoclonal antibody against norfloxacin (NOR), were employed as signal probes to establish three types of immunochromatographic strips, and the strips were evaluated according to their ability to detect NOR in milk samples. The results showed that the visual detection limit of NOR was 2.0 μg L−1 in the standard solution and 20.0 μg L−1 in milk samples when using colloidal gold-immunochromatographic strips (ICS). When QD-based fluorescence immunochromatographic strips (FICS) were used, the visual detection limit was 2.0 μg L−1 in the standard solution and 10.0 μg L−1 in milk samples. If UCNP-based FICS strips were employed, the visual limit of detection was 0.5 μg L−1 in the standard solution and 2.5 μg L−1 in milk samples. Besides, the results from the proposed methods showed high agreement with results yielded by using commercial ELISA kits, indicating the good accuracy of these strips. Compared with colloidal gold-ICS, QDs-FICS showed similar sensitivity in the standard solution and a two-fold higher sensitivity in milk samples. Meanwhile, UCNPs-FICS yielded the highest sensitivity both in standard solution and in milk samples, and they were also more stable in normal laboratory conditions (that is, they could be exposed to regular room light). Additionally, the two fluorescence nanoparticle-based test strips could be built with less antibody and coating antigens and can be cost-effective. In conclusion, the proposed QDs-FICS and UCNPs-FICS could be employed as cost-effective alternative methods for sensitive, rapid, and on-site detection of NOR in milk samples.
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Abnous K, Danesh NM, Alibolandi M, Ramezani M, Taghdisi SM, Emrani AS (2017) A novel electrochemical aptasensor for ultrasensitive detection of fluoroquinolones based on single-stranded DNA-binding protein. Sensors Actuators B Chem 240:100–106. https://doi.org/10.1016/j.snb.2016.08.100
Alhusban AA, Tarawneh OA, Dawabsheh SO, Alhusban AA, Abumhareb FW (2019) Liquid chromatography–tandem mass spectrometry for rapid and selective simultaneous determination of fluoroquinolones level in human aqueous humor. J Pharmacol Toxicol 97:36–43. https://doi.org/10.1016/j.vascn.2019.03.001
Anaya-Gonzalez C, Soldevila S, Garcia-Lainez G, Bosca F, Andreu I (2019) Chemical tuning for potential antitumor fluoroquinolones. Free Radic Biol Med 141:150–158. https://doi.org/10.1016/j.freeradbiomed.2019.06.010
Canales C, Ramos D, Fierro A, Antilén M (2019) Electrochemical, theoretical and analytical studies of the electro-oxidation of sulfamerazine and norfloxacin on a glassy carbon electrode. Electrochim Acta 318:847–856. https://doi.org/10.1016/j.electacta.2019.06.035
Caro E, Marcé RM, Cormack PAG, Sherrington DC, Borrull F (2006) Direct determination of ciprofloxacin by mass spectrometry after a two-step solid-phase extraction using a molecularly imprinted polymer. J Sep Sci 29:1230–1236. https://doi.org/10.1002/jssc.200500439
Chawla K, Kumar A, Shenoy VP, Chakrabarty S, Satyamoorthy K (2018) Genotypic detection of fluoroquinolone resistance in drug-resistant Mycobacterium tuberculosis at a tertiary care centre in south Coastal Karnataka, India. J Glob Antimicrob Resist 13:250–253. https://doi.org/10.1016/j.jgar.2018.01.023
Chen J, Shanin IA, Lv S, Wang Q, Mao C, Xu Z, Sun Y, Wu Q, Eremin SA, Lei H (2016) Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk. J Sci Food Agric 96:1341–1346. https://doi.org/10.1002/jsfa.7228
Cui J, Zhang K, Huang Q, Yu Y, Peng X (2011) An indirect competitive enzyme-linked immunosorbent assay for determination of norfloxacin in waters using a specific polyclonal antibody. Anal Chim Acta 688:84–89. https://doi.org/10.1016/j.aca.2010.12.030
Cuprys A, Pulicharla R, Brar SK, Drogui P, Verma M, Surampalli RY (2018) Fluoroquinolones metal complexation and its environmental impacts. Coord Chem Rev 376:46–61. https://doi.org/10.1016/j.ccr.2018.05.019
Di Nardo F, Anfossi L, Giovannoli C, Passini C, Goftman VV, Goryacheva IY, Baggiani C (2016) A fluorescent immunochromatographic strip test using quantum dots for fumonisins detection. Talanta 150:463–468. https://doi.org/10.1016/j.talanta.2015.12.072
Ding Y, Hua X, Chen H, Gonzalez-Sapienza G, Barnych B, Liu F, Wang M, Hammock BD (2019) A dual signal immunochromatographic strip for the detection of imidaclothiz using a recombinant fluorescent-peptide tracer and gold nanoparticles. Sensors Actuators B Chem 297:126714. https://doi.org/10.1016/j.snb.2019.126714
Duan R, Jiang J, Liu S, Yang J, Qiao M, Shi Y, Hu X (2017) Determination of norfloxacin in food by an enhanced spectrofluorimetric method. J Sci Food Agric 97:2569–2574. https://doi.org/10.1002/jsfa.8077
Hu G, Sheng W, Zhang Y, Wu X, Wang S (2015) A novel and sensitive fluorescence immunoassay for the detection of fluoroquinolones in animal-derived foods using upconversion nanoparticles as labels. Anal Bioanal Chem 407:8487–8496. https://doi.org/10.1007/s00216-015-8996-4
Hu G, Sheng W, Zhang Y, Wang J, Wu X, Wang S (2016) Upconversion nanoparticles and monodispersed magnetic polystyrene microsphere based fluorescence immunoassay for the detection of sulfaquinoxaline in animal-derived foods. J Agric Food Chem 64:3908–3915. https://doi.org/10.1021/acs.jafc.6b01497
Ibarra IS, Rodriguez JA, Páez-Hernández ME, Santos EM, Miranda JM (2012) Determination of quinolones in milk samples using a combination of magnetic solid-phase extraction and capillary electrophoresis. Electrophoresis 33:2041–2048. https://doi.org/10.1002/elps.201100559
Kurrey R, Mahilang M, Deb MK, Nirmalkar J, Shrivas K, Pervez S, Rai MK, Rai J (2019) A direct DRS-FTIR probe for rapid detection and quantification of fluoroquinolone antibiotics in poultry egg-yolk. Food Chem 270:459–466. https://doi.org/10.1016/j.foodchem.2018.07.129
Li C, Li Y, Zhou T, Xie R (2019) Ultrasonic synthesis of Mn-doped CsPbCl3 quantum dots (QDs) with enhanced photoluminescence. Opt Mater 94:41–46. https://doi.org/10.1016/j.optmat.2019.04.053
Liu Y, Zhang C, Liu H, Li Y, Xu Z, Li L, Whittaker A (2018) Controllable synthesis of up-conversion nanoparticles UCNPs@MIL-PEG for pH-responsive drug delivery and potential up-conversion luminescence/magnetic resonance dual-mode imaging. J Alloys Compd 749:939–947. https://doi.org/10.1016/j.jallcom.2018.03.355
Liu Y, Zhang F, He X, Ma P, Huang Y, Tao S, Sun Y, Wang X, Sun D (2019) A novel and simple fluorescent sensor based on AgInZnS QDs for the detection of protamine and trypsin and imaging of cells. Sensors Actuators B Chem 294:263–269. https://doi.org/10.1016/j.snb.2019.05.057
Majdinasab M, Mitsubayashi K, Marty JL (2019) Optical and electrochemical sensors and biosensors for the detection of quinolones. Trends Biotechnol 37:898–915. https://doi.org/10.1016/j.tibtech.2019.01.004
Pochivalov A, Timofeeva I, Vakh C, Bulatov A (2017) Switchable hydrophilicity solvent membrane-based microextraction: HPLC-FLD determination of fluoroquinolones in shrimps. Anal Chim Acta 976:35–44. https://doi.org/10.1016/j.aca.2017.04.054
Rafique R, Kailasa SK, Park TJ (2019) Recent advances of upconversion nanoparticles in theranostics and bioimaging applications. TrAC Trends Anal Chem:115646. https://doi.org/10.1016/j.trac.2019.115646
Sheng W, Li Y, Xu X, Yuan M, Wang S (2011) Enzyme-linked immunosorbent assay and colloidal gold-based immunochromatographic assay for several (fluoro)quinolones in milk. Microchim Acta 173:307–316. https://doi.org/10.1007/s00604-011-0560-0
Song X, Shukla S, Kim M (2019) An immunoliposome-based immunochromatographic strip assay for the rapid detection of Cronobacter species. J Microbiol Methods 159:91–98. https://doi.org/10.1016/j.mimet.2019.02.006
Szerkus O, Jacyna J, Gibas A, Sieczkowski M, Siluk D, Matuszewskib M, Kaliszan R, Markuszewski MJ (2017) Robust HPLC–MS/MS method for levofloxacin and ciprofloxacin determination in human prostate tissue. J Pharmaceut Biomed 132:173–183. https://doi.org/10.1016/j.jpba.2016.10.008
Tan X, Li Q, Yang J (2020) CdTe QDs based fluorescent sensor for the determination of gallic acid in tea. Spectrochim Acta A 224:117356. https://doi.org/10.1016/j.saa.2019.117356
Tian R, Zhao S, Liu G, Chen H, Ma L, You H, Liu C, Wang Z (2019) Construction of lanthanide-doped upconversion nanoparticle-Uelx Europaeus Agglutinin-I bioconjugates with brightness red emission for ultrasensitive in vivo imaging of colorectal tumor. Biomaterials 212:64–72. https://doi.org/10.1016/j.biomaterials.2019.05.010
Tomas A, Stilinović N, Sabo A, Tomić Z (2019) Use of microdialysis for the assessment of fluoroquinolone pharmacokinetics in the clinical practice. Eur J Pharm Sci 131:230–242. https://doi.org/10.1016/j.ejps.2019.02.032
Tumini M, Nagel O, Molina MP, Althaus R (2017) Microbiological assay with Bacillus licheniformis for the easy detection of quinolones in milk. Int Dairy J 64:9–13. https://doi.org/10.1016/j.idairyj.2016.08.008
Wagman AS, Cirz R, McEnroe G, Aggen J, Linsell MS, Goldblum AA, Lopez S, Gomez M, Miller G, Simons LJ, Belliotti TR, Harris CR, Poel T, Melnick MJ, Gaston RD, Moser HE (2017) Synthesis and microbiological evaluation of novel tetracyclic fluoroquinolones. ChemMedChem 12:1687–1692. https://doi.org/10.1002/cmdc.201700426
Wang Z, Guo L, Liu L, Kuang H, Xu C (2018) Colloidal gold-based immunochromatographic strip assay for the rapid detection of three natural estrogens in milk. Food Chem 259:122–129. https://doi.org/10.1016/j.foodchem.2018.03.087
Wangman P, Longyant S, Utari HB, Senapin S, Pengsuk C, Sithigorngul P, Chaivisuthangkura P (2016) Sensitivity improvement of immunochromatographic strip test for infectious myonecrosis virus detection. Aquaculture 453:163–168. https://doi.org/10.1016/j.aquaculture.2015.11.041
Wu W, Li M, Chen M, Li L, Wang R, Chen H, Chen F, Mi Q, Liang W, Chen H (2017a) Development of a colloidal gold immunochromatographic strip for rapid detection of Streptococcus agalactiae in tilapia. Biosens Bioelectron 91:66–69. https://doi.org/10.1016/j.bios.2016.11.038
Wu Y, Peng W, Zhao Q, Piao J, Zhang B, Wu X, Wang H, Shi Z, Gong X, Chang J (2017b) Immune fluorescence test strips based on quantum dots for rapid and quantitative detection of carcino-embryonic antigen. Chin Chem Lett 28:1881–1884. https://doi.org/10.1016/j.cclet.2017.07.026
Xu X, Feng L, Li J, Yuan P, Feng J, Wei L, Cheng X (2019) Rapid screening detection of fluoroquinolone residues in milk based on turn-on fluorescence of terbium coordination polymer nanosheets. Chin Chem Lett 30:549–552. https://doi.org/10.1016/j.cclet.2018.11.026
Yan Q, Chen Z, Xue S, Han X, Lin Z, Zhang S, Shi G, Zhang M (2018) Lanthanide-doped nanoparticles encountering porphyrin hydrate: boosting a dual-mode optical nanokit for Cu2+ sensing. Sensors Actuators B Chem 268:108–114. https://doi.org/10.1016/j.snb.2018.04.080
Yang Q, Gong X, Song T, Yang J, Zhu S, Li Y, Cui Y, Li Y, Zhang B, Chang J (2011) Quantum dot-based immunochromatography test strip for rapid, quantitative and sensitive detection of alpha fetoprotein. Biosens Bioelectron 30:145–150. https://doi.org/10.1016/j.bios.2011.09.002
Yao H, Shen H, Tang Q (2019) Highly luminescent up/down conversion thin films prepared by a room temperature process. Thin Solid Films 683:1–7. https://doi.org/10.1016/j.tsf.2019.05.010
Zhang S, Yao T, Wang S, Feng R, Chen L, Zhu V, Hu G, Zhang H, Yang G (2019) Upconversion luminescence nanoparticles-based immunochromatographic assay for quantitative detection of triamcinolone acetonide in cosmetics. Spectrochim Acta A 214:302–308. https://doi.org/10.1016/j.saa.2019.02.053
Zheng H, Mo J, Zhang Y, Gao Q, Ding J, Yu Q, Feng Y (2014) Facile synthesis of magnetic molecularly imprinted polymers and its application in magnetic solid phase extraction for fluoroquinolones in milk samples. J Chromatogr A 1329:17–23. https://doi.org/10.1016/j.chroma.2013.12.083
This study was funded by the Natural Science Foundation of Hebei Province (grant number C2019208188), the Doctoral Research Foundation Project of Hebei University of Science and Technology (grant numbers 81/1181288 and 81/1181289), and by the PT Foundation of Hebei University of Science and Technology (grant number 82/1182218).
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Gaoshuang Hu declares that she has no conflict of interest. Shan Gao declares that he has no conflict of interest. Xue Han declares that she has no conflict of interest. Lixin Yang declares that he has no conflict of interest.
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Hu, G., Gao, S., Han, X. et al. Comparison of Immunochromatographic Strips Using Colloidal Gold, Quantum Dots, and Upconversion Nanoparticles for Visual Detection of Norfloxacin in Milk Samples. Food Anal. Methods (2020). https://doi.org/10.1007/s12161-020-01725-3
- Upconversion nanoparticles
- Quantum dots
- Colloidal gold
- Immunochromatographic strips