Mepanipyrim is a new-generation anilinopyrimidine pesticide that is employed nowadays to fight fungal diseases in a variety of crops. Rapid and user-friendly analytical methods that can complement the instrumental techniques are demanded. To analyse residues of this toxic compound in foodstuffs, monoclonal antibodies were generated for the first time, and an enzyme-linked immunosorbent assay was developed. In the present study, a series of haptens mimicking the mepanipyrim molecule, and with a linker at alternative sites of the molecular framework, were designed and prepared by total synthesis in order to study the influence of the linker tethering site over the binding properties of the generated antibodies. This strategy afforded high-affinity and specific monoclonal antibodies to mepanipyrim. Interestingly, the highest sensitivity was reached with antibodies that had been raised using haptens with the spacer arm at the most characteristic moiety of the analyte, the 1-propynyl group. Moreover, the influence of the triple bond was investigated both in immunizing bioconjugates and in assay antigens. Finally, a competitive enzyme-linked immunosorbent assay was optimized and characterized, showing an IC50 value of 0.3 μg/L for mepanipyrim. Good accuracy and precision values were found when grape juice and wine samples were analysed.
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This work was supported by the Spanish Ministerio de Economía y Competitividad (AGL2012-39965-C02 and AGL2015-64488-C2) and co-financed by European Regional Development Funds (ERDF). Conjugate analysis was carried out at the Proteomics Section which belongs to ProteoRed, PRB2-3, and was supported by grant PT17/0019, of the PE I + D + i 2013–2016, and funded by ISCIII and ERDF.
Conflict of Interest
Josep V. Mercader declares that he has no conflict of interest. Consuelo Agulló declares that he has no conflict of interest. Antonio Abad-Somovilla declares that he has no conflict of interest. Antonio Abad-Fuentes declares that he has no conflict of interest.
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Mercader, J.V., Agulló, C., Abad-Somovilla, A. et al. A Monoclonal Antibody-Based Immunoassay for Mepanipyrim Residue Sensitive Analysis in Grape Juice and Wine. Food Anal. Methods 13, 770–779 (2020). https://doi.org/10.1007/s12161-019-01693-3
- Monoclonal antibody
- Grape juice