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Method Development and Validation for Determination of Indoxacarb Using LC-ESI-MS/MS and Its Dissipation Kinetics in Pigeonpea


A simple, sensitive and reproducible analytical method for quantitative determination of indoxacarb residues in pigeonpea green pod, pigeonpea dry grain and soil was developed and validated using LC-MS/MS. The developed methodology was linear having correlation coefficients (R2) value of 0.999. The LOD and LOQ were 0.0015 and 0.005 μg g−1, respectively. QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) technique involved for extraction of indoxacarb residues with 1% ethyl acetate in acetonitrile provided acceptable recoveries in the range of 83.80–99.01% at 0.005, 0.025 and 0.050 μg g−1 spiking level with precision RSD of less than 1.20 %. Initial deposit on green pods following two application (15 days interval between application) of indoxacarb 14.5% SC at 60 and 120 g a.i.ha−1 was 0.583 and 1.342 μg g−1 and dissipated to 0.005 and 0.006 μg g−1 on 7 and 10 days after application, respectively. Half-life and safe waiting period (SWP) were ranged from 1.13 to 1.23 days and 3.23 to 5.03 days for the dose 60 and 120 g a.i.ha−1 in pigeonpea green pods, respectively. Hazard index (HI) value was more than 1 in green pods drawn on 5th and 7th day after application in recommended (60 g a.i.ha−1) and double the recommended dose (120 g a.i.ha−1), respectively.

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The authors gratefully acknowledge the University of Agricultural Sciences, Raichur, for field and laboratory research facility support.

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Correspondence to Harischandra R Naik.

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Naik, H.R., Pallavi, M.S., Chawan, R. et al. Method Development and Validation for Determination of Indoxacarb Using LC-ESI-MS/MS and Its Dissipation Kinetics in Pigeonpea. Food Anal. Methods 13, 647–657 (2020). https://doi.org/10.1007/s12161-019-01681-7

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  • Pigeonpea
  • Indoxacarb
  • Dissipation
  • LC-MS/MS
  • QuEChERS
  • Half-life