Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Construction of Co@C Hybrid Nanostructure: Electrochemical Biosensor for Detection of Penicillin Sodium in Milk

  • 83 Accesses

Abstract

In this study, a cobalt-based mesoporous carbon material (Co@C) was prepared by self-assembly method and impregnation method, and carboxyl functionalization was carried out to obtain COOH-Co@C. Then, the penicillinase (Pen X) was immobilized on the carrier material by covalent binding. The methylene blue was electropolymerized onto the glassy carbon electrode, and then the immobilized enzyme material was modified onto the modified electrode to prepare a biosensor. After optimization of experimental conditions, the differential pulse method was used to detect penicillin sodium (Pen G) as an antibiotic model. Notably, measurement was performed without any pH redox probe, and a low limit of detection was obtained. The results showed that when the concentration of Pen G was 0.1–10 ng mL−1, the corresponding current and concentration of the sensor showed a good linear relationship. The linear equation is y = 0.3881x + 17.20 (r2 = 0.9974); when the concentration range is 10–100 ng mL−1, the linear equation is y = 0.1170x + 19.73 (r2 = 0.9959). The limit of detection is 0.64 ng mL−1. The sensor is not only simple to prepare but also has high sensitivity and accurate detection. Finally, using the spike method, the actual sample detection was successfully realized.

This is a preview of subscription content, log in to check access.

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

References

  1. Bilal M, Iqbal HMN, Hu H, Wang W, Zhang X (2017) Enhanced bio-catalytic performance and dye degradation potential of chitosan-encapsulated horseradish peroxidase in a packed bed reactor system. Sci Total Environ 575:1352–1360. https://doi.org/10.1016/j.scitotenv.2016.09.215

  2. Chen YP et al (2013) Immunosensor based on magnetic relaxation switch and biotin-streptavidin system for the detection of Kanamycin in milk. Biosens Bioelectron 39:112–117. https://doi.org/10.1016/j.bios.2012.06.056

  3. Feng X et al (2016) Ratiometric electrochemiluminescent aptasensor array for antibiotic based on internal standard method and spatial-resolved technique. Sensors Actuators B Chem 226:305–311. https://doi.org/10.1016/j.snb.2015.11.131

  4. Gonçalves LM, Callera WFA, Sotomayor MDPT, Bueno PR (2014) Penicillinase-based amperometric biosensor for penicillin G. Electrochem Commun 38:131–133. https://doi.org/10.1016/j.elecom.2013.11.022

  5. Granja RHMM, Niño AMM, Zucchetti RAM, Niño REM, Patel R, Salerno AG (2009) Determination of streptomycin residues in honey by liquid chromatography-tandem mass spectrometry. Anal Chim Acta 637:64–67. https://doi.org/10.1016/j.aca.2009.01.006

  6. Gremilogianni AM, Megoulas NC, Koupparis MA (2010) Hydrophilic interaction vs ion pair liquid chromatography for the determination of streptomycin and dihydrostreptomycin residues in milk based on mass spectrometric detection. J Chromatogr A 1217:6646–6651. https://doi.org/10.1016/j.chroma.2010.04.059

  7. Ikpo CO et al (2013) Electrokinetic and impedimetric dynamics of FeCo-nanoparticles on glassy carbon electrode nano hybrids. 3:1–23. https://doi.org/10.4028/www.scientific.net/NH.3.1

  8. Illing G, Hellgardt K, Schonert M, Wakeman RJ, Jungbauer A (2005) Towards ultrathin polyaniline films for gas separation. J Membr Sci 253:199–208. https://doi.org/10.1016/j.memsci.2004.12.031

  9. Joshi KA et al (2006) V-type nerve agent detection using a carbon nanotube-based amperometric enzyme electrode analytical chemistry 78:331-336. https://doi.org/10.1021/ac051052f

  10. Lee SR, Rahman MM, Sawada K, Ishida M (2009) Fabrication of a highly sensitive penicillin sensor based on charge transfer techniques. Biosens Bioelectron 24:1877–1882. https://doi.org/10.1016/j.bios.2008.09.008

  11. Li B, Cao H, Shao J, Li G, Qu M, Yin G (2011) Co3O4@graphene composites as anode materials for high-performance lithium ion batteries. Inorg Chem 50:1628–1632. https://doi.org/10.1021/ic1023086

  12. Li Z, Liu C, Sarpong V, Gu Z (2019) Multisegment nanowire/nanoparticle hybrid arrays as electrochemical biosensors for simultaneous detection of antibiotics. Biosens Bioelectron 126:632–639. https://doi.org/10.1016/j.bios.2018.10.025

  13. Liang K, Wang R, Boutter M, Doherty CM, Mulet X, Richardson JJ (2017) Biomimetic mineralization of metal–organic frameworks around polysaccharides. Chem Commun 53:1249–1252. https://doi.org/10.1039/C6CC09680H

  14. Liu B, Jin L, Zheng H, Yao H, Wu Y, Lopes A, He J (2017) Ultrafine Co-based nanoparticle@Mesoporous carbon nanospheres toward high-performance supercapacitors. ACS Appl Mater Interfaces 9:1746–1758. https://doi.org/10.1021/acsami.6b11958

  15. Ramezani M, Danesh NM, Lavaee P, Abnous K, Taghdisi SM (2016) A selective and sensitive fluorescent aptasensor for detection of kanamycin based on catalytic recycling activity of exonuclease III and gold nanoparticles. Sensors Actuators B Chem 222:1–7. https://doi.org/10.1016/j.snb.2015.08.024

  16. Shi Y, Liu W, Tao Q-L, Jiang X-P, Liu C-H, Zeng S, Zhang Y-W (2016) Immobilization of lipase by adsorption onto magnetic nanoparticles in organic solvents. 16. https://doi.org/10.1166/jnn.2016.10694

  17. Siqueira JR Jr, Abouzar MH, Poghossian A, Zucolotto V, Oliveira ON Jr, Schöning MJ (2009a) Penicillin biosensor based on a capacitive field-effect structure functionalized with a dendrimer/carbon nanotube multilayer. Biosens Bioelectron 25:497–501. https://doi.org/10.1016/j.bios.2009.07.007

  18. Siqueira JR Jr, Werner CF, Bäcker M, Poghossian A, Zucolotto V, Oliveira ON Jr, Schon̈ing MJ (2009b) Layer-by-layer assembly of carbon nanotubes incorporated in light-addressable potentiometric sensors. J Phys Chem C 113:14765–14770. https://doi.org/10.1021/jp904777t

  19. Sojitra UV, Nadar SS, Rathod VK (2017) Immobilization of pectinase onto chitosan magnetic nanoparticles by macromolecular cross-linker. Carbohydr Polym 157:677–685. https://doi.org/10.1016/j.carbpol.2016.10.018

  20. Soleimani M, Khani A, Najafzadeh K (2012) α-Amylase immobilization on the silica nanoparticles for cleaning performance towards starch soils in laundry detergents. J Mol Catal B Enzym 74:1–5. https://doi.org/10.1016/j.molcatb.2011.07.011

  21. Song-Il O, Yan J-M, Wang H-L, Wang Z-L, Jiang Q (2014) High catalytic kinetic performance of amorphous CoPt NPs induced on CeOx for H2 generation from hydrous hydrazine. Int J Hydrog Energy 39:3755–3761. https://doi.org/10.1016/j.ijhydene.2013.12.135

  22. Stred’anský M, Pizzariello A, Stred’anská S, Miertuš S (2000) Amperometric pH-sensing biosensors for urea, penicillin, and oxalacetate. Anal Chim Acta 415:151–157. https://doi.org/10.1016/S0003-2670(00)00869-2

  23. Süzer Ş (2000) XPS Investigation of X-Ray-Induced Reduction of Metal Ions. Appl Spectrosc 54:1716–1718. https://doi.org/10.1366/0003702001948772

  24. Tosa T, Mori T, Fuse N, Chibata I (1966) Studies on continuous enzyme reactions. I. Screening of carriers for preparation of water-insoluble aminoacylase, vol 31

  25. Vijayanand S, Kannan R, Potdar HS, Pillai VK, Joy PA (2013) Porous Co3O4 nanorods as superior electrode material for supercapacitors and rechargeable Li-ion batteries. J Appl Electrochem 43:995–1003. https://doi.org/10.1007/s10800-013-0593-7

  26. Wang G, Shen X, Yao J, Wexler D, Ahn J-h (2009) Hydrothermal synthesis of carbon nanotube/cobalt oxide core-shell one-dimensional nanocomposite and application as an anode material for lithium-ion batteries. Electrochem Commun 11:546–549. https://doi.org/10.1016/j.elecom.2008.12.048

  27. Wang Y-Q, Zhang Z-B, Liu Y-H, Cao X-H, Liu Y-T, Li Q (2012) Adsorption of U(VI) from aqueous solution by the carboxyl-mesoporous carbon. Chem Eng J 198-199:246–253. https://doi.org/10.1016/j.cej.2012.05.112

  28. Wei Q, Zhao Y, Du B, Wu D, Li H, Yang M (2012) Ultrasensitive detection of kanamycin in animal derived foods by label-free electrochemical immunosensor. Food Chem 134:1601–1606. https://doi.org/10.1016/j.foodchem.2012.02.126

  29. Wu Y, Tang L, Huang L, Han Z, Wang J, Pan H (2014) A low detection limit penicillin biosensor based on single graphene nanosheets preadsorbed with hematein/ionic liquids/penicillinase. Mater Sci Eng C 39:92–99. https://doi.org/10.1016/j.msec.2014.02.012

  30. Xu W, Wang Y, Liu S, Yu J, Wang H, Huang J (2014) A novel sandwich-type electrochemical aptasensor for sensitive detection of kanamycin based on GR-PANI and PAMAM-Au nanocomposites. New J Chem 38:4931–4937. https://doi.org/10.1039/c4nj00858h

  31. Xue J, Liu J, Wang C, Tian Y, Zhou N (2016) Simultaneous electrochemical detection of multiple antibiotic residues in milk based on aptamers and quantum dots. Anal Methods 8:1981–1988. https://doi.org/10.1039/c5ay03136b

  32. Yan Z, Gan N, Li T, Cao Y, Chen Y (2016) A sensitive electrochemical aptasensor for multiplex antibiotics detection based on high-capacity magnetic hollow porous nanotracers coupling exonuclease-assisted cascade target recycling. Biosens Bioelectron 78:51–57. https://doi.org/10.1016/j.bios.2015.11.019

  33. Zhang H, Noonan O, Huang X, Yang Y, Xu C, Zhou L, Yu C (2016) Surfactant-free assembly of mesoporous carbon hollow spheres with large tunable pore sizes. ACS Nano 10:4579–4586. https://doi.org/10.1021/acsnano.6b00723

  34. Zhuo L, Wu Y, Ming J, Wang L, Yu Y, Zhang X, Zhao F (2013) Facile synthesis of a Co3O4–carbon nanotube composite and its superior performance as an anode material for Li-ion batteries. J Mater Chem A 1:1141–1147. https://doi.org/10.1039/C2TA00284A

  35. Zou X, Huang X, Goswami A, Silva R, Sathe BR, Mikmeková E, Asefa T (2014) Cobalt-embedded nitrogen-rich carbon nanotubes efficiently catalyze hydrogen evolution reaction at all pH values. Angew Chem Int Ed 53:4372–4376. https://doi.org/10.1002/anie.201311111

Download references

Funding

This work was supported by the Key Program of Jilin Province (grant number: 20180201044NY), and Jilin Educational Committee (grant number: 3D518L714071).

Author information

Correspondence to Hongsu Wang.

Ethics declarations

Conflicts of Interest

Yi Xiu declares that she has no conflict of interest. Ruiping Luo declares that she has no conflict of interest. Baoqing Han declares that he has no conflict of interest. Lu Liu declares that she has no conflict of interest. Hongsu Wang declares that she has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Not applicable.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Xiu, Y., Luo, R., Han, B. et al. Construction of Co@C Hybrid Nanostructure: Electrochemical Biosensor for Detection of Penicillin Sodium in Milk. Food Anal. Methods 13, 617–628 (2020). https://doi.org/10.1007/s12161-019-01677-3

Download citation

Keywords

  • Cobalt-based mesoporous carbon
  • Enzyme biosensor
  • Penicillinase
  • Penicillin
  • Detection