Tamarindus indica L. Seed: Optimization of Maceration Extraction Recovery of Tannins
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The seeds of Tamarindus indica L., classified as bio-waste, are powerful sources of bioactive compounds, especially tannins. In order to use its full potential, extraction of such bioactive constituents should be done under the optimized conditions. In the frame of this paper, central-composite experimental design and RSM (response surface methodology) were applied in order to investigate the impact of the maceration parameters on target responses and to optimize extraction process. Extraction was performed under the different levels of extraction solvent (methanol), temperature, and solvent-to-sample ratio. Obtained extracts were evaluated in terms of total phenols, flavanols, and tannin yield and in vitro antioxidant activity (DPPH, FRAP, CUPRAC). Experimental results were fitted to a second-order polynomial model where regression analysis and analysis of variance were used to determine model fitness and optimal extraction conditions. Optimized extraction conditions determined by RSM were methanol concentration of 69.99%, extraction temperature of 23.38 °C, solvent-to-sample ratio of 1:20. Chemical characterization of the extract obtained under the optimized conditions was done by using HPLC-ESI-MS/MS technique. The study could provide a scientific baseline for designing novel functional products from T. indica seeds in further studies.
KeywordsTamarind RSM HPLC-ESI-MS/MS Antioxidant Bioactive compounds
Technical and human support provided by CICT of Universidad de Jaén (UJA, MINECO, Junta de Andalucía, FEDER) is gratefully acknowledged.
Compliance with Ethical Standards
Conflict of Interest
Cvetanović, A. declares that she has no conflict of interest. Uysal, S. declares that she has no conflict of interest. Pavlić, B. declares that he has no conflict of interest. Sinan, K.I. declares that he has no conflict of interest. Llorent-Martínez, E.J. declares that he has no conflict of interest. Zengin, G. declares that he has no conflict of interest.
This article does not contain any studies with human or animal subjects.
Informed consent is not applicable in this study.
- Doughari J (2006) Antimicrobial activity of Tamarindus indica Linn. Trop J Pharm Res 5:597–603Google Scholar
- Kajdžanoska M, Gjamovski V, Stefova M (2010) HPLC-DAD-ESI-MSn identification of phenolic compounds in cultivated strawberries from Macedonia. Maced J Chem Chem En 29:181–194Google Scholar
- Ricci A, Parpinello GP, Palma AS, Teslić N, Brilli C, Pizzi A, Versari A (2017) Analytical profiling of food-grade extracts from grape (Vitis vinifera sp.) seeds and skins, green tea (Camellia sinensis) leaves and Limousin oak (Quercus robur) heartwood using MALDI-TOF-MS, ICP-MS and spectrophotometric methods. J Food Compn Anal 59:95–104CrossRefGoogle Scholar
- Rudra SG, Nishad J, Jakhar N, Kaur C (2015) Food industry waste: mine of nutraceuticals. Int J Sci Environ Technol 4:205–229Google Scholar
- Uysal A, Ozer OY, Zengin G, Stefanucci A, Mollica A, Picot-Allain CMN, Mahomoodally MF (2019a) Multifunctional approaches to provide potential pharmacophores for the pharmacy shelf: Heracleum sphondylium L. subsp. ternatum (Velen.) Brummitt. Comput Biol Chem 78:64–73. https://doi.org/10.1016/j.compbiolchem.2018.11.018 CrossRefPubMedGoogle Scholar
- Uysal S, Cvetanović A, Zengin G, Zeković Z, Mahomoodally MF, Bera O (2019b) Optimization of maceration conditions for improving the extraction of phenolic compounds and antioxidant effects of Momordica charantia L. leaves through response surface methodology (rsm) and artificial neural networks (anns). Anal Lett 52:2150–2163CrossRefGoogle Scholar
- Zengin G, Sarikurkcu C, Uyar P, Aktumsek A, Uysal S, Kocak MS, Ceylan R (2015) Crepis foetida L. subsp. rhoeadifolia (Bieb.) Celak. as a source of multifunctional agents: cytotoxic and phytochemical evaluation. J Funct Foods 17:698–708. https://doi.org/10.1016/j.jff.2015.06.041 CrossRefGoogle Scholar
- Zengin G, Stefanucci A, Rodrigues MJ, Mollica A, Custodio L, Aumeeruddy MZ, Mahomoodally MF (2019) Scrophularia lucida L. as a valuable source of bioactive compounds for pharmaceutical applications: in vitro antioxidant, anti-inflammatory, enzyme inhibitory properties, in silico studies, and HPLC profiles. J Pharm Biomed Anal 162:225–233PubMedCrossRefPubMedCentralGoogle Scholar