A simple colorimetric method is demonstrated for the quantitative detection of aliphatic biogenic amines, putrescine and cadaverine using ninhydrin. The protocol is based on the formation of a complex equivalent to that of Ruhemann’s purple with a molar ratio of 1:2.5 between putrescine and ninhydrin which exhibited an absorption peak at 564 nm. The observation was theoretically verified using density functional theory (DFT). It is observed that the colorimetric detection protocol was rapid at 80 °C. Putrescine and cadaverine exhibited a similar colour on reaction with ninhydrin at pH 8.0, since the structure of both the biogenic amines is quite similar except for an addition of a methylene group, a distinguishable colour change was not observed. The analytical response was studied at 564 nm and 570 nm for putrescine and cadaverine respectively. Putrescine exhibited a linear range of 25–150 ppm, while cadaverine showed a non-linear range of 35–150 ppm. The detection limit for putrescine and cadaverine was observed to be 10 ppm and 40 ppm respectively. The specificity of the method was tested employing nine amino acids in comparison with that of aliphatic biogenic amines.
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Biji KB, Ravishankar CN, Venkateswarlu R, Mohan CO, Gopal TK (2016) Biogenic amines in seafood: a review. J Food Sci Technol 53:2210–2218. https://doi.org/10.1007/s13197-016-2224-x
del Rio B, Redruello B, Linares DM, Ladero V, Ruas-Madiedo P, Fernandez M, Martin MC, Alvarez MA (2019) The biogenic amines putrescine and cadaverine show in vitro cytotoxicity at concentrations that can be found in foods. Sci Rep 9:120–127. https://doi.org/10.1038/s41598-018-36239-w
YEN G-C, HSIEH C-L (1991) Simultaneous analysis of biogenic amines in canned fish by HPLC. J Food Sci 56:158–160. https://doi.org/10.1111/j.1365-2621.1991.tb08000.x
Gräfe A, Haupt K, Mohr GJ (2006) Optical sensor materials for the detection of amines in organic solvents. Anal Chim Acta 565:42–47. https://doi.org/10.1016/J.ACA.2006.02.034
Heerthana VR, Preetha R (2019) Biosensors: a potential tool for quality assurance and food safety pertaining to biogenic amines/volatile amines formation in aquaculture systems/products. Rev Aquac 11:220–233. https://doi.org/10.1111/raq.12236
Hu Y, Ma X, Zhang Y et al (2016) Detection of amines with fluorescent nanotubes: applications in the assessment of meat spoilage. ACS Sensors 1:22–25. https://doi.org/10.1021/acssensors.5b00040
Jin Y-J, Kwak G (2018) Detection of biogenic amines using a nitrated conjugated polymer. Sensors Actuators B Chem 271:183–188. https://doi.org/10.1016/J.SNB.2018.05.091
Karoum F, Cattabeni F, Costa E et al (1972) Gas chromatographic assay of picomole concentrations of biogenic amines. Anal Biochem 47:550–561. https://doi.org/10.1016/0003-2697(72)90149-2
Kim T-I, Park J, Kim Y (2011) A gold nanoparticle-based fluorescence turn-on probe for highly sensitive detection of polyamines. Chem – A Eur J 17:11978–11982. https://doi.org/10.1002/chem.201102060
Korent ŠM, Lobnik A, Mohr GJ (2007) Sol-gel-based optical sensor for the detection of aqueous amines. Anal Bioanal Chem 387:2863–2870. https://doi.org/10.1007/s00216-007-1146-x
Kovács Á, Simon-Sarkadi L, Ganzler K (1999) Determination of biogenic amines by capillary electrophoresis. J Chromatogr A 836:305–313. https://doi.org/10.1016/S0021-9673(98)00912-1
Lee B, Scopelliti R, Severin K (2011) A molecular probe for the optical detection of biogenic amines. Chem Commun 47:9639–9641. https://doi.org/10.1039/C1CC13604F
Lin J-F, Kukkola J, Sipola T et al (2015) Trifluoroacetylazobenzene for optical and electrochemical detection of amines. J Mater Chem A 3:4687–4694. https://doi.org/10.1039/C4TA05358C
Liu SF, Petty AR, Sazama GT, Swager TM (2015) Single-walled carbon nanotube/metalloporphyrin composites for the chemiresistive detection of amines and meat spoilage. Angew Chemie Int Ed 54:6554–6557. https://doi.org/10.1002/anie.201501434
Lobnik A, Oehme I, Murkovic I, Wolfbeis OS (1998) pH optical sensors based on sol–gels: chemical doping versus covalent immobilization. Anal Chim Acta 367:159–165. https://doi.org/10.1016/S0003-2670(97)00708-3
Minamiki T, Kurita R (2019) Potentiometric detection of biogenic amines utilizing affinity on a 4-mercaptobenzoic acid monolayer. Anal Methods 11:1155–1158. https://doi.org/10.1039/C8AY02616E
Moradian A, Mohr GJ, Linnhoff M et al (2000) Continuous optical monitoring of aqueous amines in transflectance mode. Sensors Actuators B Chem 62:154–161. https://doi.org/10.1016/S0925-4005(99)00392-5
Muresan L, Ronda Valera R, Frébort I et al (2008) Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination. Microchim Acta 163:219–225. https://doi.org/10.1007/s00604-008-0033-2
Nedeljko P, Turel M, Lobnik A (2017) Hybrid sol-gel based sensor layers for optical determination of biogenic amines. Sensors Actuators B Chem 246:1066–1073. https://doi.org/10.1016/J.SNB.2017.02.011
Pal AK, Hansda S, Datta SN, Illas F (2013) Theoretical investigation of Stilbene as photochromic spin coupler. J Phys Chem A 117:1773–1783. https://doi.org/10.1021/jp306715y
Pegg AE, Casero RA Jr (2011) Current status of the polyamine research field. Methods Mol Biol 720:3–35. https://doi.org/10.1007/978-1-61779-034-8_1
Qin W, Parzuchowski P, Zhang W, Meyerhoff ME (2003) Optical sensor for amine vapors based on dimer−monomer equilibrium of indium(III) octaethylporphyrin in a polymeric film. Anal Chem 75:332–340. https://doi.org/10.1021/ac0205356
Romano A, Klebanowski H, La Guerche S et al (2012) Determination of biogenic amines in wine by thin-layer chromatography/densitometry. Food Chem 135:1392–1396. https://doi.org/10.1016/J.FOODCHEM.2012.06.022
Ruiz-Capillas C, Herrero AM (2019) Impact of biogenic amines on food quality and safety. Foods (Basel, Switzerland) 8:62. https://doi.org/10.3390/foods8020062
Sánchez-Jiménez F, Ruiz-Pérez MV, Urdiales JL, Medina MA (2013) Pharmacological potential of biogenic amine-polyamine interactions beyond neurotransmission. Br J Pharmacol 170:4–16. https://doi.org/10.1111/bph.12109
Steiner M-S, Meier RJ, Duerkop A, Wolfbeis OS (2010) Chromogenic sensing of biogenic amines using a chameleon probe and the red−green−blue readout of digital camera images. Anal Chem 82:8402–8405. https://doi.org/10.1021/ac102029j
Sutarlie L, Yang K-L (2008) Colorimetric responses of transparent polymers doped with metal phthalocyanine for detecting vaporous amines. Sensors Actuators B Chem 134:1000–1004. https://doi.org/10.1016/J.SNB.2008.07.011
Suzzi G, Torriani S (2015) Editorial: biogenic amines in foods. Front Microbiol 6:472. https://doi.org/10.3389/fmicb.2015.00472
Wang Y, Costa CAB, Sobolewska EK, Fiutowski J, Brehm R, Albers J, Nebling E, Lofink F, Wagner B, Benecke W, Rubahn HG, de Oliveira Hansen R (2018) Micro-cantilevers for optical sensing of biogenic amines. Microsyst Technol 24:363–369. https://doi.org/10.1007/s00542-016-3257-9
Yao Y, Zhou Y, Dai J, Yue S, Xue M (2014) Host–guest recognition-induced color change of water-soluble pillararene modified silver nanoparticles for visual detection of spermine analogues. Chem Commun 50:869–871. https://doi.org/10.1039/C3CC48358D
Yurova NS, Danchuk A, Mobarez SN, Wongkaew N, Rusanova T, Baeumner AJ, Duerkop A (2018) Functional electrospun nanofibers for multimodal sensitive detection of biogenic amines in food via a simple dipstick assay. Anal Bioanal Chem 410:1111–1121. https://doi.org/10.1007/s00216-017-0696-9
Zhong X, Huo D, Fa H et al (2018) Rapid and ultrasensitive detection of biogenic amines with colorimetric sensor array. Sensors Actuators B Chem 274:464–471. https://doi.org/10.1016/J.SNB.2018.07.129
This work is financially supported by the SERB in the form of early career research grant (ECR) [ECR/2018/002035] (KG) and CSIR-Central Electrochemical Research Institute in the form of start-up research grant, (Project Number: IHP-0111).
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S. Sudalaimani declares that he has no conflict of interest. A. Esokkiya declares that he has no conflict of interest. Shekhar Hansda declares that he has no conflict of interest. C. Suresh declares that he has no conflict of interest. P. Tamilarasan declares that he has no conflict of interest. K. Giribabu declares that he has no conflict of interest.
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Sudalaimani, S., Esokkiya, A., Hansda, S. et al. Colorimetric Sensing of Putrescine and Cadaverine Using Ninhydrin as a Food Spoilage Detection Reagent. Food Anal. Methods 13, 629–636 (2020). https://doi.org/10.1007/s12161-019-01671-9
- Biogenic amines
- Food spoilage