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Dispersive Liquid-Liquid Microextraction Method for the Simultaneous Determination of Four Isomers of Hexachlorocyclohexane and Six Pyrethroid Pesticides in Milk by Gas Chromatography Electron Capture Detector

  • Yuanling Zhao
  • Xi’ai Hou
  • Dongmei Qin
  • Dan LiuEmail author
Article
  • 34 Downloads

Abstract

In this study, a simple and rapid dispersive liquid-liquid microextraction method was established, and the residuals of four isomers of hexachlorocyclohexane and six kinds of pyrethroid pesticides in milk were simultaneously determined by gas chromatography electron capture detector (GC-ECD). The milk sample was first extracted with acetonitrile and cleaned with primary secondary amine (PSA). Then 0.5 mL of acetonitrile was mixed with 140 μL of cyclohexane and rapidly injected into 3 mL of pure water. After vortexing and centrifugation, the floating phase was removed with a 0.1-mL pipette into the GC-ECD. The type and volume of extraction solvent, volume of disperser solvent, volume of water, vortex time, and amount of salt were optimized. Under optimal extraction conditions, the ten pesticides showed a good linear relationship in a certain concentration range in milk matrix, and the correlation coefficients were greater than 0.99. The limits of detection ranged from 0.07 to 2 μg/kg, and the limits of quantitation ranged from 0.2 to 5 μg/kg. The average recovery rates were between 70.1% and 106.3%, and the relative standard deviations were less than 15.2%. This method can be used for the determination of hexachlorocyclohexane and pyrethroid pesticides in milk and for subsequent research.

Keywords

Dispersive liquid-liquid microextraction Floating organic drop Milk Hexachlorocyclohexane and pyrethroid pesticides 

Notes

Compliance with Ethical Standards

Conflicts of Interest

Yuanling Zhao declares no conflict of interest. Xi’ai Hou declares no conflict of interest. Dongmei Qin declares no conflict of interest. Dan Liu declares no conflict of interest.

Ethical Approval

This article does not contain any studies involving animals and human participants performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Applied Chemistry, College of ScienceChina Agricultural UniversityBeijingPeople’s Republic of China
  2. 2.Institute Control of AgrochemicalsMinistry of AgricultureBeijingPeople’s Republic of China

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