Development and Validation of an Analytical Method for Total Polyphenols Quantification in Extra Virgin Olive Oils

  • Irene DiniEmail author
  • Serenella Seccia
  • Antonello Senatore
  • Daniele Coppola
  • Elena Morelli


Phenolic compounds in olive oil are considered interesting, both for their effect on human wellness and for their role in the shelf life of olive oils. Many methods have been suggested for quantitative analysis of phenolic compounds in extra virgin olive oil, which determine that results are not always comparable; therefore, it is necessary to have simple, inexpensive, robust, and validated methods for official routine analyses. In this work, solid phase extraction (SPE) was carried out to isolate polyphenols from olive oil and the traditional colorimetric assay (Folin-Ciocalteu method) was used to quantify them. SPE/colorimetric method was validated for total polyphenols official determinations in extra virgin oils. The proposed method was robust, precise, and accurate. Polyphenol recovery was excellent ≥ 95% in 100–500 ppm measuring range.


Polyphenols Olive oil Quantification SPE Folin-Ciocalteu 


Author Contributions

Irene Dini writing, reviewing, and editing; Elena Morelli investigation; Serenella Seccia investigation; Antonello Senatore investigation; Daniele Coppola investigation.

Compliance with Ethical Standards

Conflict of Interest

Irene Dini declares that she has no conflict of interest. Serenella Seccia declares that she has no conflict of interest. Antonello Senatore declares that he has no conflict of interest. Daniele Coppola declares that he has no conflict of interest. Elena Morelli declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals.

Statement of Informed Consent

Not applicable.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Dept. of PharmacyUniversity of Naples Federico IINapoliItaly
  2. 2.Laboratory for Product Analysis of Chamber of CommerceNapoliItaly

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