Development of a Novel Monoclonal Antibody–Based Indirect Competitive ELISA with Immunoaffinity Cleanup for the Detection of Triclosan in Chickens

  • Kai Yao
  • Jianyi Wang
  • Zhenhui Ren
  • Yanfang Zhang
  • Kai Wen
  • Bing Shao
  • Haiyang JiangEmail author


Triclosan (TCS) was quantitatively analyzed for the first time in chicken samples using immunoaffinity cleanup followed by indirect competitive enzyme-linked immunosorbent assay (icELISA). Monoclonal antibodies (mAbs) against TCS were prepared. The mAb 6E1 showed high sensitivity and specificity in phosphate-buffered saline buffer, with a half maximal inhibition concentration value of 1.77 ng mL−1 and a limit of detection of 0.09 ng mL−1. The average recovery in spiked chicken muscle samples was 84.4–114.8%, with a relative standard deviation below 8.1%. To validate the developed icELISA method, samples spiked with different levels of TCS were analyzed by icELISA and ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The consistency of results detected by icELISA and UPLC–MS/MS demonstrated that immunoaffinity cleanup followed by icELISA could also be used for other food matrices.


Triclosan Chicken samples Immunoaffinity cleanup icELISA Monoclonal antibody 


Funding Information

This work was finally supported by the Beijing Municipal Science and Technology Project (Z16110500060000) and National Natural Science Fund of China (31873026).

Compliance with Ethical Standards

Conflict of Interest

Kai Yao declares that he has no conflict of interest. Jianyi Wang declares that he has no conflict of interest. Zhenhui Ren declares that she has no conflict of interest. Yanfang Zhang declares that she has no conflict of interest. Kai Wen declares that he has no conflict of interest. Bing Shao declares that he has no conflict of interest. Haiyang Jiang declares that he has no conflict of interest.

Ethical Approval

All animal experiments in this study were carried out in accordance with respective Chinese laws and guidelines (GKFCZ2001545) concerning animal welfare and approved by the animal experimental ethics review committee of China Agricultural University (CAU20160620-2). This study does not contain any studies with human subjects.

Informed Consents

Not applicable.

Supplementary material

12161_2019_1644_MOESM1_ESM.docx (266 kb)
ESM 1 (DOCX 266 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kai Yao
    • 1
    • 2
  • Jianyi Wang
    • 1
  • Zhenhui Ren
    • 1
  • Yanfang Zhang
    • 1
  • Kai Wen
    • 1
  • Bing Shao
    • 1
    • 2
  • Haiyang Jiang
    • 1
    Email author
  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary MedicineChina Agricultural UniversityBeijingPeople’s Republic of China
  2. 2.Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food PoisoningBeijing Center for Disease Prevention and ControlBeijingPeople’s Republic of China

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