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Development of a Novel Monoclonal Antibody–Based Indirect Competitive ELISA with Immunoaffinity Cleanup for the Detection of Triclosan in Chickens

  • Kai Yao
  • Jianyi Wang
  • Zhenhui Ren
  • Yanfang Zhang
  • Kai Wen
  • Bing Shao
  • Haiyang JiangEmail author
Article
  • 30 Downloads

Abstract

Triclosan (TCS) was quantitatively analyzed for the first time in chicken samples using immunoaffinity cleanup followed by indirect competitive enzyme-linked immunosorbent assay (icELISA). Monoclonal antibodies (mAbs) against TCS were prepared. The mAb 6E1 showed high sensitivity and specificity in phosphate-buffered saline buffer, with a half maximal inhibition concentration value of 1.77 ng mL−1 and a limit of detection of 0.09 ng mL−1. The average recovery in spiked chicken muscle samples was 84.4–114.8%, with a relative standard deviation below 8.1%. To validate the developed icELISA method, samples spiked with different levels of TCS were analyzed by icELISA and ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The consistency of results detected by icELISA and UPLC–MS/MS demonstrated that immunoaffinity cleanup followed by icELISA could also be used for other food matrices.

Keywords

Triclosan Chicken samples Immunoaffinity cleanup icELISA Monoclonal antibody 

Notes

Funding Information

This work was finally supported by the Beijing Municipal Science and Technology Project (Z16110500060000) and National Natural Science Fund of China (31873026).

Compliance with Ethical Standards

Conflict of Interest

Kai Yao declares that he has no conflict of interest. Jianyi Wang declares that he has no conflict of interest. Zhenhui Ren declares that she has no conflict of interest. Yanfang Zhang declares that she has no conflict of interest. Kai Wen declares that he has no conflict of interest. Bing Shao declares that he has no conflict of interest. Haiyang Jiang declares that he has no conflict of interest.

Ethical Approval

All animal experiments in this study were carried out in accordance with respective Chinese laws and guidelines (GKFCZ2001545) concerning animal welfare and approved by the animal experimental ethics review committee of China Agricultural University (CAU20160620-2). This study does not contain any studies with human subjects.

Informed Consents

Not applicable.

Supplementary material

12161_2019_1644_MOESM1_ESM.docx (266 kb)
ESM 1 (DOCX 266 kb)

References

  1. Ahn KC, Ranganathan A, Bever CS, Hwang SH, Holland EB, Morisseau K, Pessah IN, Hammock BD, Gee SJ (2016) Detection of the antimicrobial triclosan in environmental samples by immunoassay. Environ Sci Technol 50:3754–3761CrossRefGoogle Scholar
  2. Aparicio I, Martín J, Abril C, Santos JL, Alonso E (2018) Determination of household and industrial chemicals, personal care products and hormones in leafy and root vegetables by liquid chromatography-tandem mass spectrometry. J Chromatogr A 1533:49–56CrossRefGoogle Scholar
  3. Armstrong DL, Rice CP, Ramirez M, Torrents A (2017) Influence of thermal hydrolysis-anaerobic digestion treatment of wastewater solids on concentrations of triclosan, triclocarban, and their transformation products in biosolids. Chemosphere 171:609–616CrossRefGoogle Scholar
  4. Brun EM, Bonet E, Puchades R, Maquieira A (2008) Selective enzyme-linked immunosorbent assay for triclosan. Application to wastewater treatment plant effluents. Environ Sci Technol 42:1665–1672CrossRefGoogle Scholar
  5. Cabrera-Peralta J, PeñA-Alvarez A (2018) Simple method for the determination of personal care product ingredients in lettuce by ultrasound-assisted extraction combined with solid-phase microextraction followed by GC-MS. J Sep Sci 41:1–8CrossRefGoogle Scholar
  6. Canosa P, Rodríguez I, Rubí E, Ramil M, Cela R (2008) Simplified sample preparation method for triclosan and methyltriclosan determination in biota and foodstuff samples. J Chromatogr A 1188:132–139CrossRefGoogle Scholar
  7. Cao XY, Hua X, Xiong JW, Zhu WT, Zhang J, Chen L (2018) Impact of triclosan on female reproduction through reducing thyroid hormones to suppress hypothalamic kisspeptin neurons in mice. Front Mol Neurosci 11:6CrossRefGoogle Scholar
  8. Carey DE, Zitomer DH, Kappell AD, Choi MJ, Hristova KR, McNamara PJ (2016) Chronic exposure to triclosan sustains microbial community shifts and alters antibiotic resistance gene levels in anaerobic digesters. Environ Sci Process Impacts 18:1060–1067CrossRefGoogle Scholar
  9. Cullinan MP, Palmer JE, Carle AD, West MJ, Westerman B, Seymour GJ (2015) The influence of a triclosan toothpaste on adverse events in patients with cardiovascular disease over 5-years. Sci Total Environ 508:546–552CrossRefGoogle Scholar
  10. Fair PA, Lee HB, Adams J, Darling C, Pacepavicius G, Alaee M, Bossart GD, Henry N, Muir D (2009) Occurrence of triclosan in plasma of wild Atlantic bottlenose dolphins (Tursiops truncatus) and in their environment. Environ Pollut 157:2248–2254CrossRefGoogle Scholar
  11. Ferrer C, Lozano A, Agüera A, Girón AJ, Fernández-Alba AR (2011) Overcoming matrix effects using the dilution approach in multiresidue methods for fruits and vegetables. J Chromatogr A 1218:7634–7639CrossRefGoogle Scholar
  12. Fitzgerald J, Leonard P, Darcy E, Sharma S, O’Kennedy R (2017) Immunoaffinity chromatography: concepts and applications. Methods Mol Biol 1485:27–51CrossRefGoogle Scholar
  13. Fu QG, Sanganyado E, Ye QF, Gan J (2015) Uptake of triclosan and triclocarban by vegetables from soils and biosolids-amended soils. Abstr Pap Am Chem Soc 250:118Google Scholar
  14. Halden RU, Paull DH (2005) Co-occurrence of triclocarban and triclosan in U.S. water resources. Environ Sci Technol 39:1420–1426CrossRefGoogle Scholar
  15. Hontela A, Habibi HR (2013) 8-personal care products in the aquatic environment: a case study on the effects of triclosan in fish. Fish Physiol 33:411–437CrossRefGoogle Scholar
  16. Kantiani L, Marinella F, Asperger D, Rubio F, González S, López de Alda MJ et al (2008) Triclosan and methyl-triclosan monitoring study in the northeast of Spain using a magnetic particle enzyme immunoassay and confirmatory analysis by gas chromatography–mass spectrometry. J Hydrol 361:1–9CrossRefGoogle Scholar
  17. Kim YJ, Cho YA, Lee HS, Lee YT, Gee SJ, Hammock BD (2003a) Synthesis of haptens for immunoassay of organophosphorus pesticides and effect of heterology in hapten spacer arm length on immunoassay sensitivity. Anal Chim Acta 475:85–96CrossRefGoogle Scholar
  18. Kim YJ, Cho YA, Lee HS, Lee YT (2003b) Investigation of the effect of hapten heterology on immunoassay sensitivity and development of an enzyme-linked immunosorbent assay for the organophosphorus insecticide fenthion. Anal Chim Acta 494:29–40CrossRefGoogle Scholar
  19. Kucuk R (2016) Feed additive used e.g. for increasing body weight gain of poultry comprises triclosan, chlorhexidine, boric acid, potassium dichromate, potassium permanganate, amino acid, organic acids, essential oil and stabilizing agent. TR201613905-AGoogle Scholar
  20. Mathews S, Henderson S, Reinhold D (2014) Uptake and accumulation of antimicrobials, triclocarban and triclosan, by food crops in a hydroponic system. Environ Sci Pollut Res Int 21:6025–6033CrossRefGoogle Scholar
  21. Mijangos L, Bizkarguenaga E, Prieto A, Fernández LA, Zuloaga O (2015) Simultaneous determination of a variety of endocrine disrupting compounds in carrot, lettuce and amended soil by means of focused ultrasonic solid–liquid extraction and dispersive solid-phase extraction as simplified clean-up strategy. J Chromatogr A 1389:8–18CrossRefGoogle Scholar
  22. Morgan CL, Newman DJ, Burrin JM, Price CP (1998) The matrix effects on kinetic rate constants of antibody–antigen interactions reflect solvent viscosity. J Immunol Methods 217:51–60CrossRefGoogle Scholar
  23. Pycke BF, Roll IB, Brownawell BJ, Kinney CA, Furlong ET, Kolpin DW et al (2014) Transformation products and human metabolites of triclocarban and triclosan in sewage sludge across the United States. Environ Sci Technol 48(14):7881–7890CrossRefGoogle Scholar
  24. Queckenberg C, Meins J, Wachall B, Doroshyenko O, Tomalik-Scharte D, Bastian B, Abdel-Tawab M, Fuhr U (2010) Absorption, pharmacokinetics, and safety of triclosan after dermal administration. Antimicrob Agents Chemother 54:570–572CrossRefGoogle Scholar
  25. Ribado JV, Ley C, Haggerty TD, Tkachenko E, Bhatt AS, Parsonnet J (2017) Household triclosan and triclocarban effects on the infant and maternal microbiome. EMBO Mol Med 9:1732–1741CrossRefGoogle Scholar
  26. Rüdel H, Heinz W, Böhmer M, Müller M, Fliedner A, Ricking M et al (2013) Retrospective study of triclosan and methyl-triclosan residues in fish and suspended particulate matter: results from the German environmental specimen bank. Chemosphere 91:1517–1524CrossRefGoogle Scholar
  27. Shelver WL, Kamp LM, Church JL, Rubio FM (2007) Measurement of triclosan in water using a magnetic particle enzyme immunoassay. J Agric Food Chem 55:3758–3763CrossRefGoogle Scholar
  28. Shi Y, Liu X, Zhang J, Shao B (2013) Analysis of triclosan and triclocarban in human nails using isotopic dilution liquid chromatography–tandem mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci 934:97–101CrossRefGoogle Scholar
  29. Sicherer SH, Leung DYM (2013) Advances in allergic skin disease, anaphylaxis, and hypersensitivity reactions to foods, drugs, and insects in 2012. J Allergy Clin Immunol 131:55–66CrossRefGoogle Scholar
  30. Spinks CA, Wyatt GM, Lee HA, Morgan MR (1999) Molecular modeling of hapten structure and relevance to broad specificity immunoassay of sulfonamide antibiotics. Bioconjug Chem 10:583–588CrossRefGoogle Scholar
  31. Syder GH (2013) Proceedings of the Florida state horticultural society, Sarasota Hyatt Regency, Sarasota, Florida, USA, 2-4th June 126:126–127Google Scholar
  32. U.S. FDA. (2017) 5 things to know about triclsan. In: For consumers. https://www.fda.gov/ForConsumers/ConsumerUpdates/ucm205999.htm. Accessed 20 Dec 2018
  33. Vermeiren L, Devlieghere F, Debevere J (2000) Study on the feasibility of a triclosan-containing film as antimicrobial food packaging material. Proceedings of the 14th forum for applied biotechnology, Brugge, 27-28th September 2000, 511–517Google Scholar
  34. Wang Z, Zhang H, Ni H, Zhang S, Shen J (2014) Development of a highly sensitive and specific immunoassay for enrofloxacin based on heterologous coating haptens. Anal Chim Acta 820:152–158CrossRefGoogle Scholar
  35. Wang C, Chen L, Zhao S, Hu Y, Zhou Y, Gao Y et al (2017a) Impacts of prenatal triclosan exposure on fetal reproductive hormones and its potential mechanism. Environ Int 111:279–286CrossRefGoogle Scholar
  36. Wang F, Guo X, Chen W, Sun Y, Fan C (2017b) Effects of triclosan on hormones and reproductive axis in female Yellow River carp (Cyprinus carpio): potential mechanisms underlying estrogen effect. Toxicol Appl Pharmacol 336:49–54CrossRefGoogle Scholar
  37. Wang J, Peng T, Zhang X, Yao K, Ke Y, Shao B, Wang Z, Shen J, Jiang H (2018) A novel hapten and monoclonal antibody-based indirect competitive ELISA for simultaneous analysis of alternariol and alternariol monomethyl ether in wheat. Food Control 94:65–70CrossRefGoogle Scholar
  38. Xu ZL, Shen YD, Beier RC, Yang JY, Lei HT, Wang H, Sun YM (2009) Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants: a review. Anal Chim Acta 647:125–136CrossRefGoogle Scholar
  39. Yao K, Wen K, Shan W, Xie S, Peng T, Wang J, Jiang H, Shao B (2018) Development of an immunoaffinity column for the highly sensitive analysis of bisphenol A in 14 kinds of foodstuffs using ultra-high-performance liquid chromatography tandem mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci 1080:50–58CrossRefGoogle Scholar
  40. Yu Y, Yu W (2017) Forecast of chicken consumption market in China based on broiler breed difference. China Poultry 39:41–45Google Scholar
  41. Zhang Y, Wang FX, Fang L, Wang S, Fang G (2009) Rapid determination of ractopamine residues in edible animal products by enzyme-linked immunosorbent assay: development and investigation of matrix effects. J Biomed Biotechnol 2009:579175PubMedPubMedCentralGoogle Scholar
  42. Zhang X, Wen K, Wang Z, Jiang H, Beier RC, Shen J (2016) An ultra-sensitive monoclonal antibody-based fluorescent microsphere immunochromatographic test strip assay for detecting aflatoxin M1 in milk. Food Control 60:588–595CrossRefGoogle Scholar
  43. Zhang X, Song M, Yu X, Wang Z, Ke Y, Jiang H, Li J, Shen J, Wen K (2017) Development of a new broad-specific monoclonal antibody with uniform affinity for aflatoxins and magnetic beads-based enzymatic immunoassay. Food Control 79:309–316CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kai Yao
    • 1
    • 2
  • Jianyi Wang
    • 1
  • Zhenhui Ren
    • 1
  • Yanfang Zhang
    • 1
  • Kai Wen
    • 1
  • Bing Shao
    • 1
    • 2
  • Haiyang Jiang
    • 1
    Email author
  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary MedicineChina Agricultural UniversityBeijingPeople’s Republic of China
  2. 2.Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food PoisoningBeijing Center for Disease Prevention and ControlBeijingPeople’s Republic of China

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