Development of a Novel Monoclonal Antibody–Based Indirect Competitive ELISA with Immunoaffinity Cleanup for the Detection of Triclosan in Chickens
- 30 Downloads
Triclosan (TCS) was quantitatively analyzed for the first time in chicken samples using immunoaffinity cleanup followed by indirect competitive enzyme-linked immunosorbent assay (icELISA). Monoclonal antibodies (mAbs) against TCS were prepared. The mAb 6E1 showed high sensitivity and specificity in phosphate-buffered saline buffer, with a half maximal inhibition concentration value of 1.77 ng mL−1 and a limit of detection of 0.09 ng mL−1. The average recovery in spiked chicken muscle samples was 84.4–114.8%, with a relative standard deviation below 8.1%. To validate the developed icELISA method, samples spiked with different levels of TCS were analyzed by icELISA and ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The consistency of results detected by icELISA and UPLC–MS/MS demonstrated that immunoaffinity cleanup followed by icELISA could also be used for other food matrices.
KeywordsTriclosan Chicken samples Immunoaffinity cleanup icELISA Monoclonal antibody
This work was finally supported by the Beijing Municipal Science and Technology Project (Z16110500060000) and National Natural Science Fund of China (31873026).
Compliance with Ethical Standards
Conflict of Interest
Kai Yao declares that he has no conflict of interest. Jianyi Wang declares that he has no conflict of interest. Zhenhui Ren declares that she has no conflict of interest. Yanfang Zhang declares that she has no conflict of interest. Kai Wen declares that he has no conflict of interest. Bing Shao declares that he has no conflict of interest. Haiyang Jiang declares that he has no conflict of interest.
All animal experiments in this study were carried out in accordance with respective Chinese laws and guidelines (GKFCZ2001545) concerning animal welfare and approved by the animal experimental ethics review committee of China Agricultural University (CAU20160620-2). This study does not contain any studies with human subjects.
- Fu QG, Sanganyado E, Ye QF, Gan J (2015) Uptake of triclosan and triclocarban by vegetables from soils and biosolids-amended soils. Abstr Pap Am Chem Soc 250:118Google Scholar
- Kantiani L, Marinella F, Asperger D, Rubio F, González S, López de Alda MJ et al (2008) Triclosan and methyl-triclosan monitoring study in the northeast of Spain using a magnetic particle enzyme immunoassay and confirmatory analysis by gas chromatography–mass spectrometry. J Hydrol 361:1–9CrossRefGoogle Scholar
- Kucuk R (2016) Feed additive used e.g. for increasing body weight gain of poultry comprises triclosan, chlorhexidine, boric acid, potassium dichromate, potassium permanganate, amino acid, organic acids, essential oil and stabilizing agent. TR201613905-AGoogle Scholar
- Mijangos L, Bizkarguenaga E, Prieto A, Fernández LA, Zuloaga O (2015) Simultaneous determination of a variety of endocrine disrupting compounds in carrot, lettuce and amended soil by means of focused ultrasonic solid–liquid extraction and dispersive solid-phase extraction as simplified clean-up strategy. J Chromatogr A 1389:8–18CrossRefGoogle Scholar
- Syder GH (2013) Proceedings of the Florida state horticultural society, Sarasota Hyatt Regency, Sarasota, Florida, USA, 2-4th June 126:126–127Google Scholar
- Thermo Fisher. Carrier proteins. In: Antibody production (immunogen preparation). https://www.thermofisher.com/cn/zh/home/life-science/antibodies/antibodies-learning-center/antibodies-resource-library/antibody-methods/antibody-production-immunogen-preparation.html. Accessed 20 Dec 2018
- U.S. FDA. (2017) 5 things to know about triclsan. In: For consumers. https://www.fda.gov/ForConsumers/ConsumerUpdates/ucm205999.htm. Accessed 20 Dec 2018
- Vermeiren L, Devlieghere F, Debevere J (2000) Study on the feasibility of a triclosan-containing film as antimicrobial food packaging material. Proceedings of the 14th forum for applied biotechnology, Brugge, 27-28th September 2000, 511–517Google Scholar
- Yao K, Wen K, Shan W, Xie S, Peng T, Wang J, Jiang H, Shao B (2018) Development of an immunoaffinity column for the highly sensitive analysis of bisphenol A in 14 kinds of foodstuffs using ultra-high-performance liquid chromatography tandem mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci 1080:50–58CrossRefGoogle Scholar
- Yu Y, Yu W (2017) Forecast of chicken consumption market in China based on broiler breed difference. China Poultry 39:41–45Google Scholar